##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062338_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2232696
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137688247750702	32.0	32.0	32.0	32.0	32.0
2	30.758955540745358	32.0	32.0	32.0	32.0	32.0
3	30.759222930484043	32.0	32.0	32.0	32.0	32.0
4	30.82797523711244	32.0	32.0	32.0	32.0	32.0
5	30.602895781602154	32.0	32.0	32.0	32.0	32.0
6	34.271175296592105	36.0	36.0	36.0	32.0	36.0
7	34.13318696320502	36.0	36.0	36.0	32.0	36.0
8	34.12349778026207	36.0	36.0	36.0	32.0	36.0
9	34.2985180248453	36.0	36.0	36.0	32.0	36.0
10	33.933840970736725	36.0	36.0	36.0	32.0	36.0
11	34.30119864056728	36.0	36.0	36.0	32.0	36.0
12	34.057432807690795	36.0	36.0	36.0	32.0	36.0
13	34.18502026249879	36.0	36.0	36.0	32.0	36.0
14	34.08081754076686	36.0	36.0	36.0	32.0	36.0
15	34.01944734079337	36.0	36.0	36.0	32.0	36.0
16	34.01718863651836	36.0	36.0	36.0	32.0	36.0
17	33.93649202578408	36.0	36.0	36.0	32.0	36.0
18	33.95813760583617	36.0	36.0	36.0	32.0	36.0
19	33.93138250796347	36.0	36.0	36.0	32.0	36.0
20	33.90754675065482	36.0	36.0	36.0	32.0	36.0
21	33.865543719342	36.0	36.0	36.0	32.0	36.0
22	33.81559289755524	36.0	36.0	36.0	32.0	36.0
23	33.771165443033894	36.0	36.0	36.0	32.0	36.0
24	33.73183944433098	36.0	36.0	36.0	27.0	36.0
25	33.2518865085081	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	12.0
4	86.0
5	213.0
6	328.0
7	103.0
8	334.0
9	398.0
10	232.0
11	87.0
12	178.0
13	113.0
14	399.0
15	467.0
16	797.0
17	1160.0
18	1504.0
19	2368.0
20	3504.0
21	5082.0
22	7506.0
23	10870.0
24	14824.0
25	20467.0
26	28411.0
27	36247.0
28	48874.0
29	64674.0
30	83721.0
31	113844.0
32	162502.0
33	232018.0
34	500135.0
35	891236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32864920165805	17.256154753724744	11.781579590970132	25.633616453647072
2	15.596101803171338	19.981259702717253	39.226165468225254	25.196473025886156
3	19.528366918497543	24.532570569604804	27.937281615062016	28.001780896835637
4	12.279408964971969	15.887347666477778	36.09157145704872	35.741671911501534
5	14.081459746616073	37.175580624826104	33.7766230321571	14.966336596400726
6	33.53829140072304	35.310188396523515	17.59431028518477	13.55720991756868
7	29.785583383418107	30.395609212371543	21.11390330927023	18.704904094940115
8	27.621068693940547	34.161519361622105	19.73391357906812	18.48349836536923
9	27.422715722614043	14.988012172531468	18.64255113879878	38.94672096605571
10	15.882570551610797	27.187822994881405	31.89603180119322	25.033574652314577
11	36.91433286175191	21.025028193992608	22.825901254884904	19.234737689370583
12	25.18095631037483	23.48337533971352	29.571624615439813	21.76404373447184
13	30.303996441349756	20.178928001456462	25.08081624746251	24.436259309731266
14	23.24113876539578	20.57974147015642	25.185000497759187	30.994119266688607
15	24.742750632334168	28.811794663238615	22.75282045027663	23.692634254150583
16	24.888140995030632	25.751626601885068	25.920664961934982	23.43956744114932
17	23.26906028070638	26.435224484835768	26.565716763594178	23.729998470863674
18	24.090303265131865	24.74307850164853	28.676547089867366	22.490071143352242
19	25.325792427461906	25.55110472088453	25.37956421086432	23.74353864078924
20	25.21670112061273	24.695811267113	26.692510863082557	23.394976749191716
21	25.650997579654565	24.791162801752765	24.452709087215403	25.105130531377263
22	24.96944224528289	26.04014111515059	25.236107715805034	23.754308923761485
23	23.161072985685063	25.840352812059763	25.944495698611743	25.054078503643428
24	24.238334918710727	25.937626275197296	26.03180711047367	23.792231695618305
25	24.451321948826223	25.414137681438902	25.75963735091806	24.374903018816816
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	361.0
1	361.0
2	869.5
3	1378.0
4	1378.0
5	1378.0
6	3425.5
7	5473.0
8	5473.0
9	5473.0
10	5456.0
11	5439.0
12	5439.0
13	5439.0
14	5638.0
15	5837.0
16	5837.0
17	5837.0
18	10091.0
19	14345.0
20	14345.0
21	14345.0
22	24603.5
23	34862.0
24	34862.0
25	34862.0
26	54955.0
27	75048.0
28	75048.0
29	75048.0
30	98531.5
31	122015.0
32	122015.0
33	122015.0
34	141633.5
35	161252.0
36	161252.0
37	161252.0
38	177147.0
39	193042.0
40	193042.0
41	193042.0
42	218896.5
43	244751.0
44	244751.0
45	244751.0
46	284029.5
47	323308.0
48	323308.0
49	323308.0
50	306403.0
51	289498.0
52	289498.0
53	289498.0
54	257408.5
55	225319.0
56	225319.0
57	225319.0
58	205699.5
59	186080.0
60	186080.0
61	186080.0
62	164185.0
63	142290.0
64	142290.0
65	142290.0
66	118335.0
67	94380.0
68	94380.0
69	94380.0
70	71707.5
71	49035.0
72	49035.0
73	49035.0
74	38542.5
75	28050.0
76	28050.0
77	28050.0
78	22947.5
79	17845.0
80	17845.0
81	17845.0
82	12576.5
83	7308.0
84	7308.0
85	7308.0
86	5389.0
87	3470.0
88	3470.0
89	3470.0
90	2311.5
91	1153.0
92	1153.0
93	1153.0
94	700.5
95	248.0
96	248.0
97	248.0
98	578.5
99	909.0
100	909.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008689046784694378
2	0.003314378670450433
3	0.005061145807579715
4	0.015273015224643211
5	0.03345730901116856
6	0.045684678971073533
7	0.06624278450805662
8	0.08586032312504703
9	0.09405669199926904
10	0.11604804236671719
11	0.11694382038575785
12	0.12881287913804657
13	0.1181083318105107
14	0.12101961037239282
15	0.1104942186486651
16	0.10897139601629599
17	0.11998946565049608
18	0.1268421674961571
19	0.12997739056279942
20	0.11940720993811967
21	0.1276035788123417
22	0.14829605105218086
23	0.13736755921988483
24	0.13181373550183278
25	0.12908161254375877
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2232696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.75939383622056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.21910022922245	32.32483149657254
2	15.288760114739652	13.686312704862654
3	5.463609760547477	7.336435831192897
4	2.4461786931168206	4.379579020759483
5	1.3223336970585626	2.9593427364774887
6	0.7544103718012949	2.026017056735024
7	0.4903409039645942	1.5363153144191772
8	0.3273233947659387	1.1720637390509878
9	0.2385853325005455	0.9611041374843796
>10	1.2206487023115675	9.906047319520765
>50	0.10905697554030012	3.402497197156372
>100	0.09742700384801603	9.227407578612695
>500	0.014616323446355668	4.400062648546746
>1k	0.0073081617231778315	5.596788277700346
>5k	2.0022360885418717E-4	0.620297033900225
>10k+	1.0011180442709359E-4	0.46489790700822325
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10375	0.46468484737734117	No Hit
TCCATGTACTCTGCGTTGATACCAC	8486	0.38007861347895106	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5357	0.23993414240004013	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3854	0.1726164242691347	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3645	0.16325554397015984	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3604	0.1614191990311265	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3276	0.1467284395188597	No Hit
GTCCTACAGTGGACATTTCTAAATT	3223	0.14435462776840197	No Hit
CATGTACTCTGCGTTGATACCACTG	3181	0.14247349392841657	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2829	0.12670780079330102	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2747	0.12303511091523432	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2705	0.12115397707524894	No Hit
CCCATGTACTCTGCGTTGATACCAC	2586	0.11582409786195702	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2510	0.11242014138960253	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2471	0.11067337425247324	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2407	0.10780688459154314	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2368	0.10606011745441385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.957780190406576E-5
2	0.0	0.0	0.0	0.0	8.957780190406576E-5
3	0.0	0.0	0.0	0.0	8.957780190406576E-5
4	0.0	0.0	0.0	0.0	8.957780190406576E-5
5	0.0	0.0	0.0	0.0	8.957780190406576E-5
6	0.0	0.0	0.0	0.0	1.3436670285609865E-4
7	0.0	0.0	0.0	4.478890095203288E-5	1.7915560380813152E-4
8	0.0	0.0	0.0	4.478890095203288E-5	1.7915560380813152E-4
9	0.0	0.0	0.0	4.478890095203288E-5	1.7915560380813152E-4
10	0.0	0.0	0.0	4.478890095203288E-5	1.7915560380813152E-4
11	0.0	0.0	0.0	4.478890095203288E-5	1.7915560380813152E-4
12	0.0	0.0	0.0	4.478890095203288E-5	4.478890095203288E-4
13	0.0	0.0	0.0	4.478890095203288E-5	4.926779104723616E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAA	30	7.764675E-4	18.985228	1
CTCGACG	100	0.0	17.10204	13
TCGACGC	110	0.0	15.547308	14
ACGCTTA	115	3.765308E-10	13.220154	17
CGTGCGC	80	1.9973322E-6	13.064351	10
AATATCG	75	1.477453E-5	12.668178	14
GACGCTT	130	1.9826984E-10	12.425116	16
ACGGTAT	390	0.0	12.181486	9
CGAATTG	55	0.0030664252	12.092351	14
CGAACGA	160	3.6379788E-12	11.876948	16
CGCAATA	185	0.0	11.803216	2
CGCATCG	250	0.0	11.7814045	13
AGGACCT	1475	0.0	11.206105	5
CCAACGA	170	1.0913936E-11	11.178806	19
TAACGAA	170	1.0913936E-11	11.177803	13
TGTAGGA	2145	0.0	11.153663	2
CGACGCT	145	1.2169039E-9	11.139009	15
CGGTCCA	350	0.0	11.130149	10
TGCGCTA	60	0.0058708214	11.084904	10
CGACTCG	60	0.005871802	11.084655	14
>>END_MODULE