FastQCFastQC Report
Thu 2 Feb 2017
SRR4062338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2232696
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA37640.16858542318345177No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC33920.15192395202929554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA25500.022.0875112
GTAGGAC25000.021.4727233
CTGTAGG25700.021.3201891
TTAGGAC13750.021.1207083
TAGGACG21150.020.4919574
CGCAATA5300.020.33939436
GCGAGAA17150.019.75487920
AGGACCT20700.019.4490595
AGGACGT22100.019.3120025
GGACGTG21550.018.88616
TATGGCG28850.018.83514416
GTCCTAC22900.018.8341241
ATCACGG14500.018.81358135
TAGGACC19100.018.775084
AATCACG14900.018.60381934
GACGTGA12550.018.406177
TAGGACA10400.018.404054
GGCGAGA17950.018.38419519
GATTTCG12600.018.33353841
GGACCTG21300.018.0749156
Produced by FastQC (version 0.11.5)