##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062338_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2232696
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23274507590823	32.0	32.0	32.0	32.0	32.0
2	31.393174888117326	32.0	32.0	32.0	32.0	32.0
3	31.498833697019208	32.0	32.0	32.0	32.0	32.0
4	31.61289759107375	32.0	32.0	32.0	32.0	32.0
5	31.533752467868442	32.0	32.0	32.0	32.0	32.0
6	35.143090684983534	36.0	36.0	36.0	36.0	36.0
7	35.164850476733065	36.0	36.0	36.0	36.0	36.0
8	35.10228306943713	36.0	36.0	36.0	36.0	36.0
9	35.22696327668433	36.0	36.0	36.0	36.0	36.0
10	35.10531035125248	36.0	36.0	36.0	36.0	36.0
11	35.24473192947002	36.0	36.0	36.0	36.0	36.0
12	35.15540181018822	36.0	36.0	36.0	36.0	36.0
13	35.186914385120055	36.0	36.0	36.0	36.0	36.0
14	35.14791265806003	36.0	36.0	36.0	36.0	36.0
15	35.10759279364499	36.0	36.0	36.0	36.0	36.0
16	35.12235835062185	36.0	36.0	36.0	36.0	36.0
17	35.097817616012215	36.0	36.0	36.0	36.0	36.0
18	35.10896736501521	36.0	36.0	36.0	36.0	36.0
19	35.07678698757019	36.0	36.0	36.0	36.0	36.0
20	35.05905013490417	36.0	36.0	36.0	36.0	36.0
21	35.03626154209977	36.0	36.0	36.0	36.0	36.0
22	35.0145698294797	36.0	36.0	36.0	36.0	36.0
23	34.968937553522736	36.0	36.0	36.0	36.0	36.0
24	34.947871541848954	36.0	36.0	36.0	36.0	36.0
25	34.9150076857754	36.0	36.0	36.0	32.0	36.0
26	34.86921193032997	36.0	36.0	36.0	32.0	36.0
27	34.83068003884093	36.0	36.0	36.0	32.0	36.0
28	34.79346852415197	36.0	36.0	36.0	32.0	36.0
29	34.76623911181818	36.0	36.0	36.0	32.0	36.0
30	34.740967422345	36.0	36.0	36.0	32.0	36.0
31	34.73315220701788	36.0	36.0	36.0	32.0	36.0
32	34.68532841013734	36.0	36.0	36.0	32.0	36.0
33	34.66543004511138	36.0	36.0	36.0	32.0	36.0
34	34.63907267267913	36.0	36.0	36.0	32.0	36.0
35	34.586430485834164	36.0	36.0	36.0	32.0	36.0
36	34.54778841364879	36.0	36.0	36.0	32.0	36.0
37	34.50193622418816	36.0	36.0	36.0	32.0	36.0
38	34.45281489284703	36.0	36.0	36.0	32.0	36.0
39	34.356134914919004	36.0	36.0	36.0	32.0	36.0
40	34.34793227559865	36.0	36.0	36.0	32.0	36.0
41	34.31800119675943	36.0	36.0	36.0	32.0	36.0
42	34.247745326726076	36.0	36.0	36.0	32.0	36.0
43	34.256343004152825	36.0	36.0	36.0	32.0	36.0
44	34.21154783275466	36.0	36.0	36.0	32.0	36.0
45	34.11540935263914	36.0	36.0	36.0	32.0	36.0
46	34.13301094282428	36.0	36.0	36.0	32.0	36.0
47	34.07844417690541	36.0	36.0	36.0	32.0	36.0
48	34.05372652613701	36.0	36.0	36.0	32.0	36.0
49	34.04491207042965	36.0	36.0	36.0	32.0	36.0
50	33.527445294836376	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	13.0
21	64.0
22	205.0
23	666.0
24	1750.0
25	4289.0
26	8971.0
27	17136.0
28	27569.0
29	40649.0
30	56840.0
31	79227.0
32	115537.0
33	179925.0
34	389477.0
35	1310377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.768192679118094	17.831446087851656	12.26897705292261	26.131384180107638
2	15.06799922422253	20.427671964378735	38.81122404874319	25.693104762655544
3	18.917632136101968	24.74278658060563	28.35454626482356	27.985035018468846
4	12.042105244299368	16.39523785423061	36.31859491528384	35.244061986186175
5	13.834530092766773	37.39152128189417	33.66065062149079	15.113298003848264
6	33.85900973673331	35.4908609791197	17.254876316747726	13.395252967399255
7	29.751027457387842	30.41394798037888	21.1036791394798	18.731345422753478
8	27.591677322697457	34.354893973426734	19.58476191329313	18.46866679058268
9	27.43338098872395	14.660885315331779	18.55407095278533	39.35166274315895
10	15.77003765850792	27.315407023616288	31.85731510245909	25.057240215416698
11	37.068011050317644	20.826704575992434	22.828365348439732	19.27691902525019
12	25.14233912722556	23.4554995395701	29.546655702343717	21.855505630860627
13	30.433207207788254	20.0262373381777	25.149236617972175	24.391318836061874
14	23.301022620186536	20.441161716597335	25.19523481924991	31.06258084396622
15	24.80543701426437	28.797023867109537	22.693819489979827	23.703719628646265
16	24.951359253566093	25.732253741664785	25.785283800391994	23.53110320437713
17	23.329553150092984	26.44457642240592	26.524345455001487	23.701524972499616
18	24.294171709896915	24.552603668390145	28.622660675703276	22.530563946009668
19	25.49670891155805	25.48049532941341	25.411654788650136	23.611140970378415
20	25.360595441564815	24.588837889260336	26.604338432101816	23.446228237073026
21	25.81058952942989	24.667442410431157	24.491153296283954	25.030814763855
22	25.032740686595933	25.953286967863065	25.25825280288942	23.755719542651573
23	23.237735903141317	25.548932770068117	26.016887207214957	25.196444119575617
24	24.374881309412476	25.76692035100166	25.96090108102491	23.89729725856095
25	24.623325342993404	25.07623070942036	25.817800542483166	24.48264340510307
26	22.99444259316987	26.2359945106723	26.877729883513023	23.891833012644803
27	24.51775790344946	25.90952821163293	25.860484365090457	23.71222951982715
28	23.458903496042453	25.62973194738558	25.956690924335422	24.954673632236542
29	23.62059142847929	25.498500467596124	26.1257242365284	24.755183867396187
30	23.725128723301335	25.47494150569536	26.736958367820783	24.06297140318252
31	24.60554414931545	25.027007707274073	25.55829365036709	24.809154493043387
32	23.66972485282367	26.107719098345676	25.767950495723557	24.454605553107093
33	23.274373224120076	25.641735372840728	26.599187708492334	24.484703694546862
34	23.746896129164025	26.101448652212394	26.873788460229246	23.277866758394335
35	24.658439841339796	25.762710194312167	26.277334666251022	23.30151529809701
36	23.398617635361017	26.208897225596317	26.413896025253774	23.97858911378889
37	24.658350263537894	25.87423455768273	25.390335271796964	24.07707990698241
38	23.906299827652308	26.336769537814376	25.456443689602164	24.30048694493115
39	24.685089237406256	25.112912819300075	25.73821066549141	24.46378727780226
40	25.47879335117723	25.406638431743506	25.794868625195726	23.319699591883534
41	23.955478040897642	25.273122718005496	26.938194899798273	23.833204341298593
42	25.147041961825522	26.55605599687553	25.909438633831027	22.387463407467923
43	23.863212904936454	25.381511858309413	26.197744789259264	24.55753044749487
44	23.34917068870997	25.99453754563989	26.294757548721364	24.36153421692877
45	23.83253250778431	26.23491957704945	25.74246561108185	24.190082304084388
46	23.497543101537023	25.131892293297824	26.452718757124245	24.91784584804091
47	24.71469470093555	24.95525588794892	26.51641781953298	23.813631591582553
48	24.097324490212728	26.567969844528765	25.034218720327356	24.30048694493115
49	23.878261975656336	25.56895340879367	26.32942415805824	24.22336045749175
50	23.317997613647357	27.431992532794435	24.72132345827645	24.528686395281756
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	38.0
2	46.0
3	153.5
4	261.0
5	290.0
6	319.0
7	325.5
8	332.0
9	439.0
10	546.0
11	826.5
12	1107.0
13	2013.5
14	2920.0
15	4046.0
16	5172.0
17	6139.5
18	7107.0
19	7740.0
20	8373.0
21	9273.5
22	10174.0
23	11273.0
24	12372.0
25	13629.5
26	14887.0
27	19492.0
28	24097.0
29	30208.0
30	36319.0
31	43737.0
32	51155.0
33	61634.0
34	72113.0
35	83751.5
36	95390.0
37	102920.5
38	110451.0
39	111298.0
40	112145.0
41	115643.0
42	119141.0
43	121363.5
44	123586.0
45	136372.5
46	149159.0
47	159004.5
48	168850.0
49	168813.5
50	168777.0
51	156709.5
52	144642.0
53	139277.5
54	133913.0
55	133990.5
56	134068.0
57	130280.0
58	126492.0
59	115198.5
60	103905.0
61	92429.5
62	80954.0
63	70707.5
64	60461.0
65	51994.5
66	43528.0
67	38884.0
68	34240.0
69	31338.0
70	28436.0
71	22349.0
72	16262.0
73	14407.5
74	12553.0
75	9107.0
76	5661.0
77	5195.0
78	4729.0
79	4007.0
80	3285.0
81	2608.5
82	1932.0
83	1640.0
84	1348.0
85	1118.5
86	889.0
87	637.5
88	386.0
89	239.5
90	93.0
91	70.0
92	47.0
93	35.5
94	24.0
95	16.5
96	9.0
97	7.0
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015317804125595245
2	0.004344523392347189
3	7.614113161845589E-4
4	2.239445047601644E-4
5	0.0
6	7.614113161845589E-4
7	0.0
8	4.478890095203287E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.3436670285609862E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2232696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.34649081142419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.60438609402654	35.82771671257975
2	15.353083751579295	15.152416125486672
3	5.3697080800416	7.949287512954091
4	2.395019409854959	4.727432132063612
5	1.255008910584413	3.0965142837204613
6	0.7117367655996333	2.107302705828905
7	0.4696966990462735	1.6224518690550245
8	0.33267264031268523	1.3132981910721715
9	0.23211678763373295	1.0308734035330773
>10	1.0999852250222046	9.661331800144913
>50	0.08436921316592101	2.9257502994439157
>100	0.07842018811933425	8.263241332553518
>500	0.009410676863571502	3.192210855491847
>1k	0.004385558150013877	3.1301727760720794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3764	0.16858542318345177	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3392	0.15192395202929554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.957780190406576E-5
2	0.0	0.0	0.0	0.0	8.957780190406576E-5
3	0.0	0.0	0.0	0.0	8.957780190406576E-5
4	0.0	0.0	0.0	0.0	8.957780190406576E-5
5	0.0	0.0	0.0	0.0	8.957780190406576E-5
6	0.0	0.0	0.0	0.0	8.957780190406576E-5
7	0.0	0.0	0.0	0.0	8.957780190406576E-5
8	0.0	0.0	0.0	0.0	8.957780190406576E-5
9	0.0	0.0	0.0	0.0	8.957780190406576E-5
10	0.0	0.0	0.0	0.0	8.957780190406576E-5
11	0.0	0.0	0.0	0.0	8.957780190406576E-5
12	0.0	0.0	0.0	0.0	1.7915560380813152E-4
13	0.0	0.0	0.0	0.0	1.7915560380813152E-4
14	0.0	0.0	0.0	1.7915560380813152E-4	1.7915560380813152E-4
15	0.0	0.0	0.0	2.687334057121973E-4	4.478890095203288E-4
16	0.0	0.0	0.0	3.1352230666423014E-4	4.478890095203288E-4
17	0.0	0.0	0.0	5.822557123764274E-4	4.478890095203288E-4
18	0.0	0.0	0.0	8.062002171365918E-4	5.374668114243946E-4
19	0.0	0.0	0.0	8.957780190406576E-4	5.822557123764274E-4
20	0.0	0.0	0.0	0.0012540892266569206	5.822557123764274E-4
21	0.0	0.0	0.0	0.001836344939033348	6.270446133284603E-4
22	0.0	0.0	0.0	0.0027769118590260386	6.270446133284603E-4
23	0.0	0.0	0.0	0.004075789986634992	7.166224152325261E-4
24	0.0	0.0	0.0	0.0061360794304285045	7.166224152325261E-4
25	0.0	0.0	0.0	0.008285946676126083	7.166224152325261E-4
26	0.0	0.0	0.0	0.009763980407543168	7.614113161845589E-4
27	0.0	0.0	0.0	0.011600325346576516	7.614113161845589E-4
28	0.0	0.0	0.0	0.016706260055108262	8.062002171365918E-4
29	0.0	0.0	0.0	0.023155861792201	8.062002171365918E-4
30	0.0	0.0	0.0	0.03654774317685883	8.062002171365918E-4
31	0.0	0.0	0.0	0.06512306198425581	8.062002171365918E-4
32	0.0	0.0	0.0	0.10158122735921057	8.062002171365918E-4
33	0.0	0.0	0.0	0.13369486934181815	8.062002171365918E-4
34	0.0	0.0	0.0	0.1760203807414892	8.509891180886247E-4
35	0.0	0.0	0.0	0.22116759290113835	8.509891180886247E-4
36	0.0	0.0	0.0	0.2849917767577852	8.509891180886247E-4
37	0.0	0.0	0.0	0.37940677996467054	8.509891180886247E-4
38	0.0	0.0	0.0	0.5018148462665764	9.405669199926904E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGGA	2550	0.0	22.087511	2
GTAGGAC	2500	0.0	21.472723	3
CTGTAGG	2570	0.0	21.320189	1
TTAGGAC	1375	0.0	21.120708	3
TAGGACG	2115	0.0	20.491957	4
CGCAATA	530	0.0	20.339394	36
GCGAGAA	1715	0.0	19.754879	20
AGGACCT	2070	0.0	19.449059	5
AGGACGT	2210	0.0	19.312002	5
GGACGTG	2155	0.0	18.8861	6
TATGGCG	2885	0.0	18.835144	16
GTCCTAC	2290	0.0	18.834124	1
ATCACGG	1450	0.0	18.813581	35
TAGGACC	1910	0.0	18.77508	4
AATCACG	1490	0.0	18.603819	34
GACGTGA	1255	0.0	18.40617	7
TAGGACA	1040	0.0	18.40405	4
GGCGAGA	1795	0.0	18.384195	19
GATTTCG	1260	0.0	18.333538	41
GGACCTG	2130	0.0	18.074915	6
>>END_MODULE