FastQCFastQC Report
Thu 2 Feb 2017
SRR4062337_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062337_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617267
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG140222.2716263788603634No Hit
TCCATGTACTCTGCGTTGATACCAC120071.9451874148464117No Hit
GAGTACATGGAAGCAGTGGTATCAA54500.8829242450997704No Hit
CATGTACTCTGCGTTGATACCACTG47200.7646609975909938No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36840.5968243887977164No Hit
CCCATGTACTCTGCGTTGATACCAC36360.5890481752628928No Hit
GTATCAACGCAGAGTACTTTTTTTT36280.5877521396737554No Hit
GCTTCCATGTACTCTGCGTTGATAC34990.5668535657989168No Hit
GTACATGGGAAGCAGTGGTATCAAC34670.5616694234423677No Hit
GCGTTGATACCACTGCTTCCATGTA34020.5511391342806273No Hit
CATGGAAGCAGTGGTATCAACGCAG30740.49800167512599897No Hit
TATCAACGCAGAGTACTTTTTTTTT27470.4450262204200127No Hit
GTATCAACGCAGAGTACATGGAAGC25040.40565913939996795No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22870.37050417404461927No Hit
ACGCAGAGTACATGGAAGCAGTGGT22810.3695321473527663No Hit
ACTCTGCGTTGATACCACTGCTTCC20870.3381032843161873No Hit
CAGTGGTATCAACGCAGAGTACATG20130.3261149551166675No Hit
TATCAACGCAGAGTACATGGAAGCA16560.26827936695141646No Hit
GTATCAACGCAGAGTACATGGGAAG16160.26179918900573007No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15210.24640876638472492No Hit
GTACATGGGGTGGTATCAACGCAAA14210.23020832152050894No Hit
ATACCACTGCTTCCATGTACTCTGC13020.21092979213209193No Hit
GGTATCAACGCAGAGTACTTTTTTT12240.19829344513800348No Hit
GGTATCAACGCAGAGTACATGGAAG11260.18241700917107184No Hit
AAGCAGTGGTATCAACGCAGAGTAC11170.1809589691332924No Hit
GCGTTGATACCACTGCTTCCCATGT11160.18079696468465023No Hit
GTACTCTGCGTTGATACCACTGCTT10900.17658484901995408No Hit
GAGTACATGGGAAGCAGTGGTATCA10770.174478791187606No Hit
AAAAAGTACTCTGCGTTGATACCAC10420.16880863548513042No Hit
TATCAACGCAGAGTACATGGGAAGC9810.15892636411795866No Hit
GTACATGGGTGGTATCAACGCAAAA9430.1527701950695566No Hit
ACGCAGAGTACATGGGAAGCAGTGG9240.14969211054535558No Hit
CCACTGCTTCCATGTACTCTGCGTT8950.14499398153473295No Hit
GCTTCCCATGTACTCTGCGTTGATA8430.13656975020534062No Hit
GAAGCAGTGGTATCAACGCAGAGTA8340.13511171016756118No Hit
GTGGTATCAACGCAGAGTACATGGA8300.13446369237299255No Hit
GGTATCAACGCAGAGTACATGGGAA8170.1323576345406445No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7790.12620146549224243No Hit
ACATGGAAGCAGTGGTATCAACGCA7660.12409540765989434No Hit
CTGCTTCCATGTACTCTGCGTTGAT7020.11372712294679611No Hit
GTACATGGGAGTGGTATCAACGCAA6790.11000102062802644No Hit
TGGAAGCAGTGGTATCAACGCAGAG6750.10935300283345781No Hit
GTTGATACCACTGCTTCCATGTACT6730.10902899393617348No Hit
GTATCAACGCAGAGTACATGGGGTG6590.10676093165518324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCC250.00603562818.9958614
ATAAGCC307.7352516E-418.992783
CCGATAA453.5325196E-516.889329
TATAAGC350.002176071316.2768842
ATAACGA508.6924236E-515.20162312
TAACGAA456.7486713E-414.77935513
TTGCGCG400.005262073614.254992518
AACGAAC400.005267745314.25267814
ACGAACG400.005267745314.25267815
AATTCCG602.5666071E-514.2480535
GGTTAAT801.2939381E-714.2411191
GCTTTAA400.00529618214.2411191
GATAACG551.9553589E-413.81965711
AGAACCG551.9594021E-413.8162955
ATTCCGA551.9594021E-413.8162956
CGAACGA500.00149472213.30357916
AAAAGTG500.001499300713.2981825
TTCCGAC500.001499300713.2981827
TCCGATA655.436626E-513.1552518
CGTCAAT604.073741E-412.67110419
Produced by FastQC (version 0.11.5)