##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062337_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	617267
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83315971856587	32.0	32.0	32.0	32.0	32.0
2	30.676409074193177	32.0	32.0	32.0	32.0	32.0
3	30.696664490406906	32.0	32.0	32.0	32.0	32.0
4	30.670538032974385	32.0	32.0	32.0	32.0	32.0
5	30.59166616715295	32.0	32.0	32.0	32.0	32.0
6	34.149460444183795	36.0	36.0	36.0	32.0	36.0
7	33.95707530128777	36.0	36.0	36.0	32.0	36.0
8	33.93264503043254	36.0	36.0	36.0	32.0	36.0
9	33.99485635875561	36.0	36.0	36.0	32.0	36.0
10	33.82523284089381	36.0	36.0	36.0	32.0	36.0
11	34.143916651951265	36.0	36.0	36.0	32.0	36.0
12	33.94046822525747	36.0	36.0	36.0	32.0	36.0
13	34.00558591338918	36.0	36.0	36.0	32.0	36.0
14	33.917497614484496	36.0	36.0	36.0	32.0	36.0
15	33.93230806117936	36.0	36.0	36.0	32.0	36.0
16	33.880102127604424	36.0	36.0	36.0	32.0	36.0
17	33.81201003779564	36.0	36.0	36.0	32.0	36.0
18	33.844964982738425	36.0	36.0	36.0	32.0	36.0
19	33.83058546787695	36.0	36.0	36.0	32.0	36.0
20	33.76638310488006	36.0	36.0	36.0	32.0	36.0
21	33.65987328012027	36.0	36.0	36.0	27.0	36.0
22	33.61870632967581	36.0	36.0	36.0	27.0	36.0
23	33.53943107277726	36.0	36.0	36.0	27.0	36.0
24	33.56018870278178	36.0	36.0	36.0	27.0	36.0
25	32.97232640008295	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	29.0
5	69.0
6	114.0
7	24.0
8	93.0
9	115.0
10	82.0
11	24.0
12	33.0
13	43.0
14	249.0
15	313.0
16	399.0
17	536.0
18	755.0
19	1076.0
20	1611.0
21	2263.0
22	3066.0
23	4166.0
24	5222.0
25	6581.0
26	8625.0
27	10711.0
28	13901.0
29	18008.0
30	23231.0
31	30818.0
32	43921.0
33	61266.0
34	132308.0
35	247609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.71923707558603	16.934271326822252	12.444507812651896	23.901983784939826
2	13.819494397677417	21.772589121967975	37.64896864125046	26.758947839104145
3	19.786725250797108	27.502300593617957	27.330080099541178	25.380894056043758
4	12.291768274404896	16.86918000327305	33.99194051471172	36.84711120761033
5	13.094022579421804	38.444334237677104	33.314209217823674	15.14743396507742
6	33.17211063421466	33.23208288489218	18.889638091780235	14.706168389112928
7	27.878058824101604	31.739950909662756	22.42770199506503	17.95428827117061
8	29.399726927480113	31.608306334179794	21.271441219680682	17.720525518659418
9	27.31288677849998	16.168314326859456	18.682942176208826	37.835856718431735
10	15.747045523111908	28.49744210263381	32.21409779375269	23.541414580501588
11	35.11388594211785	22.408977216323272	20.820478382595773	21.656658458963104
12	23.234181445359393	25.341159064805623	29.58285476973791	21.841804720097073
13	31.388760472679788	20.683228302391786	24.88284386481423	23.045167360114196
14	25.01042229348854	19.64144066105728	25.33550052152021	30.012636523933967
15	26.240896631145283	25.605080044766677	26.60438259289897	21.549640731189072
16	25.305093652884196	25.66257712495053	26.579642257011805	22.45268696515347
17	23.31786335919593	25.62732880967714	28.423117040276548	22.631690790850385
18	25.263412331347162	23.22213912713386	30.210454083114197	21.30399445840478
19	23.15607778500593	29.677474826294535	26.282046082440946	20.88440130625859
20	23.669470286228737	23.039426407014187	31.26276430940929	22.02833899734778
21	24.436585444988808	26.67710327374193	24.39521754647805	24.491093734791214
22	23.03537063059613	27.083407705014327	25.518357094409303	24.362864569980236
23	21.63210606839867	26.68518407351584	25.675704177935838	26.007005680149653
24	21.580229047388052	29.108065005459306	25.855033996940197	23.456671950212453
25	24.972826527798055	25.089794130530002	24.64625817232037	25.291121169351566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	432.0
1	432.0
2	795.0
3	1158.0
4	1158.0
5	1158.0
6	2973.0
7	4788.0
8	4788.0
9	4788.0
10	4389.0
11	3990.0
12	3990.0
13	3990.0
14	3191.5
15	2393.0
16	2393.0
17	2393.0
18	3759.0
19	5125.0
20	5125.0
21	5125.0
22	7573.5
23	10022.0
24	10022.0
25	10022.0
26	14412.5
27	18803.0
28	18803.0
29	18803.0
30	23196.5
31	27590.0
32	27590.0
33	27590.0
34	31345.0
35	35100.0
36	35100.0
37	35100.0
38	39547.5
39	43995.0
40	43995.0
41	43995.0
42	55463.5
43	66932.0
44	66932.0
45	66932.0
46	100288.5
47	133645.0
48	133645.0
49	133645.0
50	111164.5
51	88684.0
52	88684.0
53	88684.0
54	72094.0
55	55504.0
56	55504.0
57	55504.0
58	49650.5
59	43797.0
60	43797.0
61	43797.0
62	38082.5
63	32368.0
64	32368.0
65	32368.0
66	26607.0
67	20846.0
68	20846.0
69	20846.0
70	15683.5
71	10521.0
72	10521.0
73	10521.0
74	8094.0
75	5667.0
76	5667.0
77	5667.0
78	4590.0
79	3513.0
80	3513.0
81	3513.0
82	2388.0
83	1263.0
84	1263.0
85	1263.0
86	922.5
87	582.0
88	582.0
89	582.0
90	405.0
91	228.0
92	228.0
93	228.0
94	137.0
95	46.0
96	46.0
97	46.0
98	160.5
99	275.0
100	275.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010206280264456063
2	0.0037261023187696732
3	0.00567015570247559
4	0.01717247155606893
5	0.039691089917329135
6	0.051031401322280304
7	0.07209197964576107
8	0.0941245846610948
9	0.10287282488777143
10	0.12069331423840898
11	0.12522943880038945
12	0.13592173241077202
13	0.1266874788381689
14	0.12960355891372777
15	0.1193972786492717
16	0.11907326975198738
17	0.13025157670829643
18	0.13575972796212984
19	0.13770378134583575
20	0.12992756781101208
21	0.13721776799990928
22	0.1608704175016646
23	0.14742404826436534
24	0.14385995039423782
25	0.13883781248633087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	617267.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.59800799581663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.55376666802199	41.264581379575404
2	11.973042074184391	12.116241572076616
3	3.0719420024349295	4.663024380056625
4	1.1728697934120416	2.373795007404571
5	0.5527933662772297	1.3985121583464832
6	0.29527465274385245	0.8964185544297256
7	0.20765713545842085	0.7354926180219488
8	0.14932002423304466	0.6044236624063302
9	0.11672513935949702	0.5315453581162177
>10	0.7093757600012314	7.141579725155197
>50	0.10199507848210007	3.64280417382681
>100	0.07915075380490243	7.50229455481789
>500	0.006756771666272152	2.5072768361163607
>1k	0.008365526824908378	9.497241009578607
>5k	3.217510317272453E-4	0.8872578895418587
>10k+	6.435020634544906E-4	4.237511120529365
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	14022	2.2716263788603634	No Hit
TCCATGTACTCTGCGTTGATACCAC	12007	1.9451874148464117	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5450	0.8829242450997704	No Hit
CATGTACTCTGCGTTGATACCACTG	4720	0.7646609975909938	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3684	0.5968243887977164	No Hit
CCCATGTACTCTGCGTTGATACCAC	3636	0.5890481752628928	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3628	0.5877521396737554	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3499	0.5668535657989168	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3467	0.5616694234423677	No Hit
GCGTTGATACCACTGCTTCCATGTA	3402	0.5511391342806273	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3074	0.49800167512599897	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2747	0.4450262204200127	No Hit
GTATCAACGCAGAGTACATGGAAGC	2504	0.40565913939996795	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2287	0.37050417404461927	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2281	0.3695321473527663	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2087	0.3381032843161873	No Hit
CAGTGGTATCAACGCAGAGTACATG	2013	0.3261149551166675	No Hit
TATCAACGCAGAGTACATGGAAGCA	1656	0.26827936695141646	No Hit
GTATCAACGCAGAGTACATGGGAAG	1616	0.26179918900573007	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1521	0.24640876638472492	No Hit
GTACATGGGGTGGTATCAACGCAAA	1421	0.23020832152050894	No Hit
ATACCACTGCTTCCATGTACTCTGC	1302	0.21092979213209193	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1224	0.19829344513800348	No Hit
GGTATCAACGCAGAGTACATGGAAG	1126	0.18241700917107184	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1117	0.1809589691332924	No Hit
GCGTTGATACCACTGCTTCCCATGT	1116	0.18079696468465023	No Hit
GTACTCTGCGTTGATACCACTGCTT	1090	0.17658484901995408	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1077	0.174478791187606	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1042	0.16880863548513042	No Hit
TATCAACGCAGAGTACATGGGAAGC	981	0.15892636411795866	No Hit
GTACATGGGTGGTATCAACGCAAAA	943	0.1527701950695566	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	924	0.14969211054535558	No Hit
CCACTGCTTCCATGTACTCTGCGTT	895	0.14499398153473295	No Hit
GCTTCCCATGTACTCTGCGTTGATA	843	0.13656975020534062	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	834	0.13511171016756118	No Hit
GTGGTATCAACGCAGAGTACATGGA	830	0.13446369237299255	No Hit
GGTATCAACGCAGAGTACATGGGAA	817	0.1323576345406445	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	779	0.12620146549224243	No Hit
ACATGGAAGCAGTGGTATCAACGCA	766	0.12409540765989434	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	702	0.11372712294679611	No Hit
GTACATGGGAGTGGTATCAACGCAA	679	0.11000102062802644	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	675	0.10935300283345781	No Hit
GTTGATACCACTGCTTCCATGTACT	673	0.10902899393617348	No Hit
GTATCAACGCAGAGTACATGGGGTG	659	0.10676093165518324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.620044486421597E-4
7	0.0	0.0	0.0	0.0	1.620044486421597E-4
8	0.0	0.0	0.0	0.0	1.620044486421597E-4
9	0.0	0.0	0.0	0.0	1.620044486421597E-4
10	0.0	0.0	0.0	0.0	1.620044486421597E-4
11	0.0	0.0	0.0	0.0	1.620044486421597E-4
12	0.0	0.0	0.0	0.0	3.240088972843194E-4
13	0.0	0.0	0.0	0.0	3.240088972843194E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCC	25	0.006035628	18.995861	4
ATAAGCC	30	7.7352516E-4	18.99278	3
CCGATAA	45	3.5325196E-5	16.88932	9
TATAAGC	35	0.0021760713	16.276884	2
ATAACGA	50	8.6924236E-5	15.201623	12
TAACGAA	45	6.7486713E-4	14.779355	13
TTGCGCG	40	0.0052620736	14.2549925	18
AACGAAC	40	0.0052677453	14.252678	14
ACGAACG	40	0.0052677453	14.252678	15
AATTCCG	60	2.5666071E-5	14.248053	5
GGTTAAT	80	1.2939381E-7	14.241119	1
GCTTTAA	40	0.005296182	14.241119	1
GATAACG	55	1.9553589E-4	13.819657	11
AGAACCG	55	1.9594021E-4	13.816295	5
ATTCCGA	55	1.9594021E-4	13.816295	6
CGAACGA	50	0.001494722	13.303579	16
AAAAGTG	50	0.0014993007	13.298182	5
TTCCGAC	50	0.0014993007	13.298182	7
TCCGATA	65	5.436626E-5	13.155251	8
CGTCAAT	60	4.073741E-4	12.671104	19
>>END_MODULE