FastQCFastQC Report
Thu 2 Feb 2017
SRR4062337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617267
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA66731.0810556857891318No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC58850.95339618025911No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA24570.3980449303137864No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC21450.3474995423374326No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG15940.2582350911356026No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC15070.24414070410373467No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA15050.24381669520645036No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG14930.24187264182274443No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13780.22324213022889608No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13600.2203260501533372No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11870.19229928053824358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.17707086236588057No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10890.17642284457131194No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA8790.14240191035645838No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA8470.13721776799990928No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC8390.13592173241077202No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG8110.13138560784879152No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7560.12247536317347274No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA7510.12166534093026193No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT7130.11550917188185988No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA7020.11372712294679611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.11048703397395293No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6510.10546489606604596No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6300.10206280264456061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG250.002352010535.1996739
TCGTATA454.0666113E-529.33306544
TAGCGTC300.00574312129.33306325
TGGTATA3850.026.85689544
GAATCAC701.1308584E-625.14262837
AAAGTAC3350.024.9549943
TAATACC450.001397514424.444224
GTCCTAC2350.024.3500631
TAGGACG2650.023.2450684
CGGTGAT1153.5106495E-1022.9563143
ATTTCGT1153.5106495E-1022.9563142
GACGTGA1750.022.6283657
ACCGCAA500.00257915921.99979622
GAACCGT500.00257915921.9997966
AAAAGTA4000.021.44982
AAGTACT4100.020.9266384
AGGACGT2950.020.8811655
CGAGCCG1900.020.84191315
AGTACTC4150.020.674515
AAAAAGT3750.020.5414661
Produced by FastQC (version 0.11.5)