##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062337_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	617267
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.070473555203826	32.0	32.0	32.0	32.0	32.0
2	31.409488924565867	32.0	32.0	32.0	32.0	32.0
3	31.496723460026214	32.0	32.0	32.0	32.0	32.0
4	31.611710977583446	32.0	32.0	32.0	32.0	32.0
5	31.554821495398265	32.0	32.0	32.0	32.0	32.0
6	35.12554372743075	36.0	36.0	36.0	36.0	36.0
7	35.144903259043495	36.0	36.0	36.0	36.0	36.0
8	35.09904627981084	36.0	36.0	36.0	36.0	36.0
9	35.159316794839185	36.0	36.0	36.0	36.0	36.0
10	35.095551843853634	36.0	36.0	36.0	36.0	36.0
11	35.171608072357664	36.0	36.0	36.0	36.0	36.0
12	35.100719785765314	36.0	36.0	36.0	36.0	36.0
13	35.1088475489537	36.0	36.0	36.0	36.0	36.0
14	35.06345876257762	36.0	36.0	36.0	36.0	36.0
15	35.05319902084511	36.0	36.0	36.0	36.0	36.0
16	35.04796141702051	36.0	36.0	36.0	36.0	36.0
17	35.03264227635691	36.0	36.0	36.0	36.0	36.0
18	35.029220742401584	36.0	36.0	36.0	36.0	36.0
19	34.982313974341736	36.0	36.0	36.0	36.0	36.0
20	34.936649780402966	36.0	36.0	36.0	36.0	36.0
21	34.868235301741386	36.0	36.0	36.0	32.0	36.0
22	34.84818401113294	36.0	36.0	36.0	32.0	36.0
23	34.74787733671167	36.0	36.0	36.0	32.0	36.0
24	34.728047992197865	36.0	36.0	36.0	32.0	36.0
25	34.71084311975207	36.0	36.0	36.0	32.0	36.0
26	34.62299620747586	36.0	36.0	36.0	32.0	36.0
27	34.5929249417189	36.0	36.0	36.0	32.0	36.0
28	34.515618038871345	36.0	36.0	36.0	32.0	36.0
29	34.46587619296026	36.0	36.0	36.0	32.0	36.0
30	34.437225706217895	36.0	36.0	36.0	32.0	36.0
31	34.39041776087171	36.0	36.0	36.0	32.0	36.0
32	34.31626022450577	36.0	36.0	36.0	32.0	36.0
33	34.22443772305988	36.0	36.0	36.0	32.0	36.0
34	34.163250262852216	36.0	36.0	36.0	32.0	36.0
35	34.013274644521736	36.0	36.0	36.0	32.0	36.0
36	33.96747436684611	36.0	36.0	36.0	32.0	36.0
37	33.843715928439394	36.0	36.0	36.0	32.0	36.0
38	33.742881119515545	36.0	36.0	36.0	32.0	36.0
39	33.5060192752893	36.0	36.0	36.0	21.0	36.0
40	33.30913688889897	36.0	36.0	36.0	14.0	36.0
41	33.35581199059726	36.0	36.0	36.0	21.0	36.0
42	33.083795181015674	36.0	36.0	36.0	14.0	36.0
43	33.10347872152569	36.0	36.0	36.0	14.0	36.0
44	33.10997185982727	36.0	36.0	36.0	14.0	36.0
45	32.77529496959986	36.0	36.0	36.0	14.0	36.0
46	32.94311537794828	36.0	36.0	36.0	14.0	36.0
47	32.747114295758564	36.0	36.0	36.0	14.0	36.0
48	32.77631883771529	36.0	36.0	36.0	14.0	36.0
49	32.8095686307546	36.0	36.0	36.0	14.0	36.0
50	32.1629424543998	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	20.0
22	100.0
23	315.0
24	893.0
25	1982.0
26	4410.0
27	8307.0
28	13852.0
29	18926.0
30	24742.0
31	31942.0
32	44508.0
33	53435.0
34	95095.0
35	318733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.45528195771971	17.325294450286552	13.045091523039956	24.17433206895378
2	13.514862152261928	22.267063487563167	37.2704900370845	26.947584323090407
3	19.084347889480206	27.669315447984253	27.921719196779343	25.324617465756198
4	12.205940722380177	17.17398524134691	34.23359497136562	36.38647906490729
5	12.863153222187481	38.24665825323563	33.58903035477354	15.301158169803342
6	33.24347442909355	33.45699733015371	18.440732004458386	14.858796236294358
7	27.648813236411474	31.502251051813886	22.58633622079262	18.262599490982023
8	29.29526444796174	31.631206592933044	21.437400671022427	17.63612828808279
9	27.444363622225065	15.479039054412432	18.707139698056107	38.36945762530639
10	15.796405769302424	28.141792773629565	32.55852005696077	23.503281400107248
11	35.46795795012531	22.035845104306564	20.98978237942414	21.506414566143985
12	23.135693306138187	24.973471771534847	30.048261125250498	21.84257379707647
13	31.786730863629515	20.52126551395101	24.960349411194834	22.73165421122464
14	24.986270122977576	19.192991039533947	25.846027731921517	29.97471110556696
15	26.148976050882354	25.497070149546307	26.844623153351794	21.509330646219546
16	25.2069201820282	25.149408602760232	27.022342033512242	22.62132918169933
17	23.3121161507095	25.20789220872005	28.844082058493324	22.635909582077122
18	25.463859237574667	22.420929678728978	30.793967602350357	21.321243481346
19	23.254604571441533	29.064570113095307	26.913961057370635	20.766864258092525
20	23.554636810326812	22.348513690185932	32.12856025026447	21.968289249222785
21	24.387501680796156	26.03071280337358	25.112147579572536	24.46963793625773
22	22.883614384050986	26.42535564026588	26.38323448361892	24.307795492064212
23	21.53087723788895	25.601563018920498	26.55333915469319	26.31422058849736
24	21.519212917586717	28.34478434777819	26.661396121937507	23.474606612697585
25	25.078288649806325	23.898734259242758	25.511002532129535	25.511974558821386
26	21.495884276982245	24.63682652725644	27.96828600913381	25.899003186627507
27	25.341059865503908	26.249742817937783	27.114684569238275	21.29451274732004
28	23.56662513952633	23.145251568608074	27.74633991449405	25.54178337737154
29	21.819245156471997	26.553177150244544	29.680997040178724	21.946580653104732
30	25.23770102727021	24.30261134970766	28.663933111603246	21.79575451141888
31	21.640554249619694	25.250337374264298	27.77598672859557	25.33312164752044
32	21.726092598502756	26.5014977311277	27.757356217001718	24.01505345336783
33	21.57542846126555	25.608691214660755	27.759138265936784	25.056742058136916
34	21.16863529072508	27.078071563845143	29.439124398355982	22.314168747073797
35	24.506898959445426	25.675437047501326	27.875781468959136	21.94188252409411
36	23.146061590851286	26.805256072331744	27.63423283603368	22.41444950078329
37	24.60037552631195	26.509435949111165	27.590977648246223	21.299210876330665
38	23.150273706515982	26.566785523930488	25.65324243803735	24.629698331516185
39	22.428867896712443	26.037678994665193	28.103073710404086	23.430379398218275
40	24.263244268687618	26.1925552475671	25.932052094150503	23.61214838959478
41	21.303260987546718	26.323940855415888	28.170305556590584	24.20249260044681
42	25.499824225173224	26.634017370116986	26.89792261695506	20.968235787754733
43	23.996584946222622	24.263568277584902	25.94063832992854	25.799208446263933
44	23.07526564679466	25.448144806056373	29.22155242382956	22.255037123319408
45	24.259032153022922	25.511164536578175	26.981192903557133	23.24861040684177
46	22.264469450771628	25.268684813354376	29.078063590089197	23.388782145784802
47	24.115172202628685	24.563114503124257	29.08465866472693	22.237054629520124
48	22.288572044188335	30.102856624442907	24.99307430982055	22.61549702154821
49	21.492320179112117	25.82431913580347	30.123917202766386	22.559443482318027
50	21.321081476897355	30.052959254261125	24.130562625249688	24.49539664359183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50.0
1	52.0
2	54.0
3	254.5
4	455.0
5	508.5
6	562.0
7	495.5
8	429.0
9	579.0
10	729.0
11	1026.5
12	1324.0
13	2281.5
14	3239.0
15	4055.5
16	4872.0
17	5469.0
18	6066.0
19	6166.0
20	6266.0
21	6580.0
22	6894.0
23	6822.5
24	6751.0
25	6168.0
26	5585.0
27	5978.5
28	6372.0
29	7255.0
30	8138.0
31	9521.0
32	10904.0
33	12625.5
34	14347.0
35	15889.0
36	17431.0
37	19301.0
38	21171.0
39	22351.0
40	23531.0
41	26268.5
42	29006.0
43	33053.0
44	37100.0
45	46249.0
46	55398.0
47	62933.0
48	70468.0
49	64416.5
50	58365.0
51	49226.0
52	40087.0
53	36293.5
54	32500.0
55	32351.5
56	32203.0
57	30657.5
58	29112.0
59	26876.5
60	24641.0
61	21660.5
62	18680.0
63	16065.5
64	13451.0
65	11580.5
66	9710.0
67	8300.5
68	6891.0
69	6293.5
70	5696.0
71	4424.0
72	3152.0
73	2777.0
74	2402.0
75	1740.0
76	1078.0
77	929.5
78	781.0
79	665.5
80	550.0
81	449.0
82	348.0
83	293.5
84	239.0
85	187.0
86	135.0
87	107.0
88	79.0
89	48.0
90	17.0
91	10.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01620044486421597
2	0.0045361245619804725
3	3.240088972843194E-4
4	3.240088972843194E-4
5	0.0
6	4.860133459264792E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.620044486421597E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	617267.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.34256941251289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.30765141464077	48.02039865839593
2	11.451024793172406	13.361644176801343
3	2.8840228779016415	5.047839148237551
4	1.1168355586509067	2.606362244118125
5	0.549786133904233	1.6037967839672411
6	0.3081029820304907	1.078531176918967
7	0.2122106218539759	0.866663905491168
8	0.14922805768114533	0.6965078650845359
9	0.11867605250951087	0.6231479248025246
>10	0.7313097134771982	8.298229247401514
>50	0.09632524355754775	3.945468872132569
>100	0.06700885130165891	7.371954544804426
>500	0.004188053206352938	1.695894881694171
>1k	0.003071239017992155	2.737931147009473
>5k	5.584070941803917E-4	2.0456294231404666
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6673	1.0810556857891318	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5885	0.95339618025911	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2457	0.3980449303137864	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2145	0.3474995423374326	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1594	0.2582350911356026	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1507	0.24414070410373467	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1505	0.24381669520645036	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1493	0.24187264182274443	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1378	0.22324213022889608	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1360	0.2203260501533372	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1187	0.19229928053824358	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1093	0.17707086236588057	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1089	0.17642284457131194	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	879	0.14240191035645838	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	847	0.13721776799990928	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	839	0.13592173241077202	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	811	0.13138560784879152	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	756	0.12247536317347274	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	751	0.12166534093026193	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	713	0.11550917188185988	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	702	0.11372712294679611	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.11048703397395293	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	651	0.10546489606604596	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	630	0.10206280264456061	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.620044486421597E-4
13	0.0	0.0	0.0	0.0	3.240088972843194E-4
14	0.0	0.0	0.0	0.0	3.240088972843194E-4
15	0.0	0.0	0.0	0.0	3.240088972843194E-4
16	0.0	0.0	0.0	3.240088972843194E-4	3.240088972843194E-4
17	0.0	0.0	0.0	6.480177945686388E-4	3.240088972843194E-4
18	0.0	0.0	0.0	8.100222432107986E-4	3.240088972843194E-4
19	0.0	0.0	0.0	0.001134031140495118	3.240088972843194E-4
20	0.0	0.0	0.0	0.001782048935063757	4.860133459264791E-4
21	0.0	0.0	0.0	0.002268062280990236	4.860133459264791E-4
22	0.0	0.0	0.0	0.004860133459264791	4.860133459264791E-4
23	0.0	0.0	0.0	0.009072249123960943	4.860133459264791E-4
24	0.0	0.0	0.0	0.015066413723720853	4.860133459264791E-4
25	0.0	0.0	0.0	0.020736569426196443	4.860133459264791E-4
26	0.0	0.0	0.0	0.026892738474598513	6.480177945686388E-4
27	0.0	0.0	0.0	0.035964987598559456	6.480177945686388E-4
28	0.0	0.0	0.0	0.05621554367882942	6.480177945686388E-4
29	0.0	0.0	0.0	0.08100222432107985	6.480177945686388E-4
30	0.0	0.0	0.0	0.12458142100582081	6.480177945686388E-4
31	0.0	0.0	0.0	0.20104752076492022	6.480177945686388E-4
32	0.0	0.0	0.0	0.29970822998799546	6.480177945686388E-4
33	0.0	0.0	0.0	0.38735263670340386	6.480177945686388E-4
34	0.0	0.0	0.0	0.49443757725587145	6.480177945686388E-4
35	0.0	0.0	0.0	0.6097847446890892	6.480177945686388E-4
36	0.0	0.0	0.0	0.7860455848117589	6.480177945686388E-4
37	0.0	0.0	0.0	1.0504368449957635	6.480177945686388E-4
38	0.0	0.0	0.0	1.3324865900817637	6.480177945686388E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTACG	25	0.0023520105	35.199673	9
TCGTATA	45	4.0666113E-5	29.333065	44
TAGCGTC	30	0.005743121	29.333063	25
TGGTATA	385	0.0	26.856895	44
GAATCAC	70	1.1308584E-6	25.142628	37
AAAGTAC	335	0.0	24.954994	3
TAATACC	45	0.0013975144	24.44422	4
GTCCTAC	235	0.0	24.350063	1
TAGGACG	265	0.0	23.245068	4
CGGTGAT	115	3.5106495E-10	22.95631	43
ATTTCGT	115	3.5106495E-10	22.95631	42
GACGTGA	175	0.0	22.628365	7
ACCGCAA	50	0.002579159	21.999796	22
GAACCGT	50	0.002579159	21.999796	6
AAAAGTA	400	0.0	21.4498	2
AAGTACT	410	0.0	20.926638	4
AGGACGT	295	0.0	20.881165	5
CGAGCCG	190	0.0	20.841913	15
AGTACTC	415	0.0	20.67451	5
AAAAAGT	375	0.0	20.541466	1
>>END_MODULE