FastQCFastQC Report
Thu 2 Feb 2017
SRR4062336_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062336_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780102
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAAGGTGTGCGAGAGAACTCT48690.6241491497265742No Hit
GTACATGGAAGCAGTGGTATCAACG46660.5981269116089948No Hit
TCCATGTACTCTGCGTTGATACCAC43140.5530046070898421No Hit
CCTTAGGATACTCTCCTCGACTACC40860.5237776598444819No Hit
GTATCAACGCAGAGTACTTTTTTTT30760.3943074110821405No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25830.33111054708230464No Hit
TATCAACGCAGAGTACTTTTTTTTT22720.29124396553271237No Hit
GAGTACATGGAAGCAGTGGTATCAA19600.25124919561800896No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18050.23137999902576842No Hit
CATGTACTCTGCGTTGATACCACTG16510.21163899079863915No Hit
GGCCTATTCACTGCGGCTGACCTTT16250.20830609330574718No Hit
TCCTTAGCCTGCTGCGTCCCTCCAT15480.19843558919218257No Hit
GAATATAACTACCCGTACCGCAAAC14590.18702682469728318No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13820.17715632058371855No Hit
GGCTAAGGAAGCGCACTGATGGATA13740.1761308136628287No Hit
TTCCAGCACCGGGCAGGCGTCAGCC13390.1716442208839357No Hit
GTCCTAAGGTAGCGAAATTCCTTGT13060.16741400483526514No Hit
GTTATATTCTAACTAGAAGCTTTTC12940.16587574445393038No Hit
GCTTCCATGTACTCTGCGTTGATAC12740.1633119771517058No Hit
ATTCAGACTCGCTTTCGCTACGGCT12420.15920994946814646No Hit
CATGGAAGCAGTGGTATCAACGCAG11550.14805756170346956No Hit
GAATAGGCCCAAACGACTGTTTATC11420.14639111295702356No Hit
CGTTGAGACAGTGCCCAAATCGTTA11390.1460065478616899No Hit
GCGTTGATACCACTGCTTCCATGTA11290.1447246642105776No Hit
AAGCTAGACCGTAAGGACTAGTGGA10470.1342132182714568No Hit
GGTATCAACGCAGAGTACTTTTTTT10170.13036756731811994No Hit
GTCTACGCCTCTCGGCCTCGACTTA10140.12998300222278625No Hit
GCTTAGAAGTGAGAATGCCGGTATG9870.12652191636478308No Hit
GACTAGTGGAGCGCTTAGAAGTGAG9820.12588097453922692No Hit
AGTTATATTTGTTTGAACGATGGAG9680.12408633742766971No Hit
GTGCTGGAAGGTTAAGAGGATGAGT9520.12203532358589006No Hit
GTATCAACGCAGAGTACATGGAAGC9270.11883061445810933No Hit
CCATTTAAAGAGTGCGTAATAGCTC9270.11883061445810933No Hit
AATATAACTAGTACAGGAATCTCAA9140.11716416571166334No Hit
GTACATGGGGTGGTATCAACGCAAA8790.11267757293277032No Hit
CCCATGTACTCTGCGTTGATACCAC8730.11190844274210296No Hit
ATTCTGGGCTTCGCCGAAGCTAACT8630.11062655909099067No Hit
TTTAAAGAGTGCGTAATAGCTCACT8530.10934467543987837No Hit
ACTTAATTTCGCTCCCCATCACAAC8360.10716547323298749No Hit
GGGTGATAGCGTGCCTTTTGTAGAA8300.1063963430423201No Hit
GTATTTAGCCTTGGGAGATGGTCCT8300.1063963430423201No Hit
GTACTGGTTCACTATCGGTCACTAG8280.10613996631209766No Hit
GTATAGGGGCTGACGCCTGCCCGGT8190.10498627102609659No Hit
ATTCAGACTCGCTTTCGCTGCGGCT8140.10434532920054045No Hit
CTTTAAATGGTGGCTGCTTCTGAGC8060.1033198222796506No Hit
GGCGTAGACGATGGATAACAGGTTG7890.10114062007275972No Hit
CTTCTAAGCGCTCCACTAGTCCTTA7840.10049967824720357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA250.006014859319.00796317
CCGATTT350.002163757616.29149418
TAACGTG350.002163757616.29149418
ACGGGTC350.002168403216.2862648
TGGACTG350.002171194916.2831295
GTCTTAG350.0021777216.2758161
TGCGAAT1100.015.545979512
GTCTTCG551.1265063E-515.54597959
GCGAGCT508.6977765E-515.201488515
GTGTAAA1200.015.0325241
CGGTCGA456.7519536E-414.77922610
GCCCATT851.710032E-814.53083615
ATAACCT1058.913048E-1114.4738915
TCTCCTC5850.014.4534812
TAGGATA5850.014.4497694
CGTGTGA400.00527254714.25139610
AGTCCTA602.5626488E-514.25139510
TCTTCGG602.5626488E-514.25139510
TCCCTTC400.00527479314.2504818
AGCGGTA400.00527479314.2504817
Produced by FastQC (version 0.11.5)