##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062336_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	780102
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.999219332856473	32.0	32.0	32.0	32.0	32.0
2	30.794430728289377	32.0	32.0	32.0	32.0	32.0
3	30.848526218366317	32.0	32.0	32.0	32.0	32.0
4	30.72855857310967	32.0	32.0	32.0	32.0	32.0
5	30.80019279530113	32.0	32.0	32.0	32.0	32.0
6	34.25577809055739	36.0	36.0	36.0	32.0	36.0
7	34.12198661200715	36.0	36.0	36.0	32.0	36.0
8	34.10458632332695	36.0	36.0	36.0	32.0	36.0
9	34.10536186293587	36.0	36.0	36.0	32.0	36.0
10	33.9669773952637	36.0	36.0	36.0	32.0	36.0
11	34.22504493002197	36.0	36.0	36.0	32.0	36.0
12	34.0774462826656	36.0	36.0	36.0	32.0	36.0
13	34.142696724274515	36.0	36.0	36.0	32.0	36.0
14	34.05619265173016	36.0	36.0	36.0	32.0	36.0
15	34.04953326616263	36.0	36.0	36.0	32.0	36.0
16	34.031724056597724	36.0	36.0	36.0	32.0	36.0
17	34.02828219899449	36.0	36.0	36.0	32.0	36.0
18	34.06457360704113	36.0	36.0	36.0	32.0	36.0
19	33.99276504867312	36.0	36.0	36.0	32.0	36.0
20	33.93031039530728	36.0	36.0	36.0	32.0	36.0
21	33.879020948542625	36.0	36.0	36.0	32.0	36.0
22	33.844827984032854	36.0	36.0	36.0	32.0	36.0
23	33.82475368605644	36.0	36.0	36.0	32.0	36.0
24	33.820936236543425	36.0	36.0	36.0	32.0	36.0
25	33.238638024258364	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	28.0
5	78.0
6	138.0
7	32.0
8	104.0
9	152.0
10	87.0
11	27.0
12	59.0
13	45.0
14	330.0
15	548.0
16	871.0
17	1250.0
18	1629.0
19	1945.0
20	2192.0
21	2485.0
22	2885.0
23	3699.0
24	4468.0
25	5598.0
26	7568.0
27	9926.0
28	13692.0
29	18517.0
30	25393.0
31	37150.0
32	56856.0
33	88277.0
34	206139.0
35	287930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90066612310201	19.78177930249373	11.606387397504731	24.71116717689953
2	16.384559571942436	20.625908558331048	38.10740409341582	24.882127776310696
3	18.276052149166098	25.902290820054613	29.821810863127666	25.99984616765162
4	12.422498012769557	15.985922715967076	37.883022641606196	33.708556629657174
5	13.003558490687015	38.36591542974385	34.35604141954926	14.274484660019876
6	29.96534671242645	38.04179427592327	17.998932955763834	13.99392605588644
7	28.295877267246468	32.54932142948767	21.24541419738834	17.909387105877524
8	28.388141392861588	32.57255102211011	20.23602256558428	18.803285019444022
9	28.703423321699855	14.17830014370766	20.35003079449805	36.768245740094436
10	17.137554770100337	26.233690895136597	31.00404791790196	25.624706416861105
11	36.31290866973389	22.75383243177341	21.40925687078497	19.524002027707727
12	22.20813176347368	23.85055442675008	30.60160982065042	23.33970398912582
13	29.343496049733826	21.615065940440562	26.370272642802504	22.671165367023104
14	23.00603733289525	19.679215352075076	26.988187157319754	30.32656015770992
15	26.54073199527745	25.984549047790157	24.035085467891793	23.439633489040602
16	24.45737752253842	26.942924059161342	23.440726362732203	25.15897205556803
17	22.752550499832513	26.219228118450488	26.894569928423007	24.133651453293993
18	21.682282575213062	28.41038607659924	27.390004107197864	22.517327240989836
19	25.258538476249885	26.48625128515154	26.210802075752383	22.04440816284619
20	24.601466434111213	25.19273739073295	27.235554802616633	22.970241372539206
21	25.435567797632647	24.584840418021866	25.09671440563318	24.882877378712305
22	25.1282498960136	24.534500377432124	27.013228097382623	23.32402162917165
23	23.607670169367076	27.265480386846004	27.070876411071374	22.05597303271555
24	22.647302318715944	26.706370853361918	26.660420102811592	23.985906725110546
25	23.55878143791353	25.897588931030718	26.081643916494997	24.461985714560754
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	192.0
1	192.0
2	477.0
3	762.0
4	762.0
5	762.0
6	2005.5
7	3249.0
8	3249.0
9	3249.0
10	3078.5
11	2908.0
12	2908.0
13	2908.0
14	2409.0
15	1910.0
16	1910.0
17	1910.0
18	3427.5
19	4945.0
20	4945.0
21	4945.0
22	7631.0
23	10317.0
24	10317.0
25	10317.0
26	18149.5
27	25982.0
28	25982.0
29	25982.0
30	31028.0
31	36074.0
32	36074.0
33	36074.0
34	41340.0
35	46606.0
36	46606.0
37	46606.0
38	55207.5
39	63809.0
40	63809.0
41	63809.0
42	80480.5
43	97152.0
44	97152.0
45	97152.0
46	121259.5
47	145367.0
48	145367.0
49	145367.0
50	133316.5
51	121266.0
52	121266.0
53	121266.0
54	103912.5
55	86559.0
56	86559.0
57	86559.0
58	71576.5
59	56594.0
60	56594.0
61	56594.0
62	46299.0
63	36004.0
64	36004.0
65	36004.0
66	27907.5
67	19811.0
68	19811.0
69	19811.0
70	15845.5
71	11880.0
72	11880.0
73	11880.0
74	8465.5
75	5051.0
76	5051.0
77	5051.0
78	3590.5
79	2130.0
80	2130.0
81	2130.0
82	1509.0
83	888.0
84	888.0
85	888.0
86	571.0
87	254.0
88	254.0
89	254.0
90	162.0
91	70.0
92	70.0
93	70.0
94	56.0
95	42.0
96	42.0
97	42.0
98	161.0
99	280.0
100	280.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008460432097341116
2	0.002563767302224581
3	0.004102027683559329
4	0.015638980543569943
5	0.03550817713581044
6	0.04845520201204458
7	0.06691432658806155
8	0.0878090301011919
9	0.09511576691253194
10	0.1199843097441104
11	0.11498496350477246
12	0.1293420603972301
13	0.11716416571166334
14	0.12177894685566758
15	0.11049837072587944
16	0.11242119620254788
17	0.12062525156966654
18	0.12588097453922692
19	0.12959843712745256
20	0.11959974464877669
21	0.12870111857167396
22	0.14792937333835832
23	0.13895618778057228
24	0.12908568366700765
25	0.12652191636478308
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	780102.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.580708232940445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72284760383157	21.35631905699583
2	13.34333163395845	7.627237365710618
3	4.359337880538681	3.73778892157443
4	1.9053302262122496	2.178227491310989
5	1.0298315618288618	1.4716657698852031
6	0.5845398198779391	1.0023937225480164
7	0.42414967304737317	0.8485748636724839
8	0.32849967815266	0.7511002764716812
9	0.2752629266806978	0.7080488455325672
>10	2.1426011627691732	13.066325813805596
>50	0.40988098647134513	8.35916668808375
>100	0.41839217634881815	23.719108270960295
>500	0.043899821501267866	8.418188235277315
>1k	0.012094848780961552	6.755854678171209
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCCTAAGGTGTGCGAGAGAACTCT	4869	0.6241491497265742	No Hit
GTACATGGAAGCAGTGGTATCAACG	4666	0.5981269116089948	No Hit
TCCATGTACTCTGCGTTGATACCAC	4314	0.5530046070898421	No Hit
CCTTAGGATACTCTCCTCGACTACC	4086	0.5237776598444819	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3076	0.3943074110821405	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2583	0.33111054708230464	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2272	0.29124396553271237	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1960	0.25124919561800896	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1805	0.23137999902576842	No Hit
CATGTACTCTGCGTTGATACCACTG	1651	0.21163899079863915	No Hit
GGCCTATTCACTGCGGCTGACCTTT	1625	0.20830609330574718	No Hit
TCCTTAGCCTGCTGCGTCCCTCCAT	1548	0.19843558919218257	No Hit
GAATATAACTACCCGTACCGCAAAC	1459	0.18702682469728318	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1382	0.17715632058371855	No Hit
GGCTAAGGAAGCGCACTGATGGATA	1374	0.1761308136628287	No Hit
TTCCAGCACCGGGCAGGCGTCAGCC	1339	0.1716442208839357	No Hit
GTCCTAAGGTAGCGAAATTCCTTGT	1306	0.16741400483526514	No Hit
GTTATATTCTAACTAGAAGCTTTTC	1294	0.16587574445393038	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1274	0.1633119771517058	No Hit
ATTCAGACTCGCTTTCGCTACGGCT	1242	0.15920994946814646	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1155	0.14805756170346956	No Hit
GAATAGGCCCAAACGACTGTTTATC	1142	0.14639111295702356	No Hit
CGTTGAGACAGTGCCCAAATCGTTA	1139	0.1460065478616899	No Hit
GCGTTGATACCACTGCTTCCATGTA	1129	0.1447246642105776	No Hit
AAGCTAGACCGTAAGGACTAGTGGA	1047	0.1342132182714568	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1017	0.13036756731811994	No Hit
GTCTACGCCTCTCGGCCTCGACTTA	1014	0.12998300222278625	No Hit
GCTTAGAAGTGAGAATGCCGGTATG	987	0.12652191636478308	No Hit
GACTAGTGGAGCGCTTAGAAGTGAG	982	0.12588097453922692	No Hit
AGTTATATTTGTTTGAACGATGGAG	968	0.12408633742766971	No Hit
GTGCTGGAAGGTTAAGAGGATGAGT	952	0.12203532358589006	No Hit
GTATCAACGCAGAGTACATGGAAGC	927	0.11883061445810933	No Hit
CCATTTAAAGAGTGCGTAATAGCTC	927	0.11883061445810933	No Hit
AATATAACTAGTACAGGAATCTCAA	914	0.11716416571166334	No Hit
GTACATGGGGTGGTATCAACGCAAA	879	0.11267757293277032	No Hit
CCCATGTACTCTGCGTTGATACCAC	873	0.11190844274210296	No Hit
ATTCTGGGCTTCGCCGAAGCTAACT	863	0.11062655909099067	No Hit
TTTAAAGAGTGCGTAATAGCTCACT	853	0.10934467543987837	No Hit
ACTTAATTTCGCTCCCCATCACAAC	836	0.10716547323298749	No Hit
GGGTGATAGCGTGCCTTTTGTAGAA	830	0.1063963430423201	No Hit
GTATTTAGCCTTGGGAGATGGTCCT	830	0.1063963430423201	No Hit
GTACTGGTTCACTATCGGTCACTAG	828	0.10613996631209766	No Hit
GTATAGGGGCTGACGCCTGCCCGGT	819	0.10498627102609659	No Hit
ATTCAGACTCGCTTTCGCTGCGGCT	814	0.10434532920054045	No Hit
CTTTAAATGGTGGCTGCTTCTGAGC	806	0.1033198222796506	No Hit
GGCGTAGACGATGGATAACAGGTTG	789	0.10114062007275972	No Hit
CTTCTAAGCGCTCCACTAGTCCTTA	784	0.10049967824720357	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.2818836511122904E-4	0.0	0.0	0.0	0.0
6	1.2818836511122904E-4	0.0	0.0	0.0	0.0
7	1.2818836511122904E-4	0.0	0.0	0.0	0.0
8	1.2818836511122904E-4	0.0	0.0	0.0	0.0
9	1.2818836511122904E-4	0.0	0.0	0.0	0.0
10	1.2818836511122904E-4	0.0	0.0	0.0	0.0
11	1.2818836511122904E-4	0.0	0.0	0.0	0.0
12	1.2818836511122904E-4	0.0	0.0	0.0	0.0
13	1.2818836511122904E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCCGA	25	0.0060148593	19.007963	17
CCGATTT	35	0.0021637576	16.291494	18
TAACGTG	35	0.0021637576	16.291494	18
ACGGGTC	35	0.0021684032	16.286264	8
TGGACTG	35	0.0021711949	16.283129	5
GTCTTAG	35	0.00217772	16.275816	1
TGCGAAT	110	0.0	15.5459795	12
GTCTTCG	55	1.1265063E-5	15.5459795	9
GCGAGCT	50	8.6977765E-5	15.2014885	15
GTGTAAA	120	0.0	15.032524	1
CGGTCGA	45	6.7519536E-4	14.779226	10
GCCCATT	85	1.710032E-8	14.530836	15
ATAACCT	105	8.913048E-11	14.473891	5
TCTCCTC	585	0.0	14.45348	12
TAGGATA	585	0.0	14.449769	4
CGTGTGA	40	0.005272547	14.251396	10
AGTCCTA	60	2.5626488E-5	14.251395	10
TCTTCGG	60	2.5626488E-5	14.251395	10
TCCCTTC	40	0.005274793	14.250481	8
AGCGGTA	40	0.005274793	14.250481	7
>>END_MODULE