FastQCFastQC Report
Thu 2 Feb 2017
SRR4062336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780102
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAAGGTGTGCGAGAGAACTCTCGTTAAGGAACTCGGCAAAATGACC45580.584282568176982No Hit
CCTTAGGATACTCTCCTCGACTACCTGTGTCGGTTTGCGGTACGGGTAGT41870.536724684720716No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30270.38802618119169036No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23900.3063701926158374No Hit
TCCTTAGCCTGCTGCGTCCCTCCATCGTTCAAACAAATATAACTAGTACA15410.19753827063640397No Hit
GAATATAACTACCCGTACCGCAAACCGACACAGGTAGTCGAGGAGAGTAT14040.17997646461616557No Hit
GGCCTATTCACTGCGGCTGACCTTTCGGTCAGCACCCCTTCTCCCGAAGT14010.17959189952083188No Hit
TTCCAGCACCGGGCAGGCGTCAGCCCCTATACGTCATCTTACGATTTTGC13090.16779856993059883No Hit
GTTATATTCTAACTAGAAGCTTTTCTTGGCAGTGTGACATCAGTTACTTC12800.1640811073423732No Hit
ATTCAGACTCGCTTTCGCTACGGCTCCGTCTTATCAACTTAACCTCGCAA12400.15895357273792401No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC11400.14613473622680112No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA11080.14203270854324176No Hit
GGCTAAGGAAGCGCACTGATGGATATGTGCGTCCAAGCAATAAGTCTGAA10890.1395971296061284No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA10690.13703336230390384No Hit
GAATAGGCCCAAACGACTGTTTATCAAAAACACAGGTTTCTGCAAAATCG10490.13446959500167927No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10340.13254676952501082No Hit
AAGCTAGACCGTAAGGACTAGTGGAGCGCTTAGAAGTGAGAATGCCGGTA10300.1320340160645659No Hit
GACTAGTGGAGCGCTTAGAAGTGAGAATGCCGGTATGAGTAGCGAAAGAC9750.12498365598344832No Hit
GTCTACGCCTCTCGGCCTCGACTTAGGTCCCGACTAACCCTGGGAGGACG9640.12357358396722479No Hit
AATATAACTAGTACAGGAATCTCAACCTGTTATCCATCGTCTACGCCTCT9610.12318901887189111No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA9470.1213943817603339No Hit
ATTCAGACTCGCTTTCGCTGCGGCTCCGTCTTATCAACTTAACCTTGCTA8980.11511315186988368No Hit
TTTAAAGAGTGCGTAATAGCTCACTAGTCGAGTGATTCTGCGCCGAAAAT8930.11447221004432753No Hit
CCATTTAAAGAGTGCGTAATAGCTCACTAGTCGAGTGATTCTGCGCCGAA8920.11434402167921631No Hit
TTTCTTGGCAGTGTGACATCAGTTACTTCGCTACTTAATTTCGCTCCCCA8380.10742184996320994No Hit
GCTTAGAAGTGAGAATGCCGGTATGAGTAGCGAAAGACAGGTGAGAATCC8200.10511445939120781No Hit
ACTTAATTTCGCTCCCCATCACAACTTGTCCTTAAAGTTAAGCATTTAAC8030.10293525718431693No Hit
GTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCTCGT7940.10178156189831586No Hit
ATTCTGGGCTTCGCCGAAGCTAACTCATCCTCTTAACCTTCCAGCACCGG7890.10114062007275972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG300.005743904529.3332047
GTGTAAT407.0221623E-427.5051691
TAGGATA6800.026.5292954
GATACTC7250.025.1860967
CGCGAAT701.1314514E-625.14274613
ACTCTCC7250.024.57920310
ATACTCT7250.024.5792038
TACTCTC7250.024.5792039
GTATGAC551.5929584E-423.9998973
AATGACC8450.023.69220244
CGCGTGG752.062785E-623.46656410
TGTGCGA8950.023.35185210
TCTCCTC7650.023.29401612
AAATGAC8700.023.01139543
TTGCGGT7850.022.98079335
GGTGTGC9100.022.966938
ACTCTCG9300.022.94613621
CTCCTCG7700.022.85704213
GTGTGCG9250.022.832339
AGAGTTA1550.022.70957840
Produced by FastQC (version 0.11.5)