##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062336_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	780102
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.120761131236684	32.0	32.0	32.0	32.0	32.0
2	31.24788168726654	32.0	32.0	32.0	32.0	32.0
3	31.38421257732963	32.0	32.0	32.0	32.0	32.0
4	31.523435396909633	32.0	32.0	32.0	32.0	32.0
5	31.430935954529023	32.0	32.0	32.0	32.0	32.0
6	34.983779044278826	36.0	36.0	36.0	36.0	36.0
7	34.99953724000195	36.0	36.0	36.0	36.0	36.0
8	34.931884548430844	36.0	36.0	36.0	36.0	36.0
9	35.054821036223466	36.0	36.0	36.0	36.0	36.0
10	34.958599003720025	36.0	36.0	36.0	32.0	36.0
11	35.06965371195049	36.0	36.0	36.0	36.0	36.0
12	34.991047324580634	36.0	36.0	36.0	36.0	36.0
13	35.00878090301012	36.0	36.0	36.0	36.0	36.0
14	34.97497763113029	36.0	36.0	36.0	32.0	36.0
15	34.94360993818757	36.0	36.0	36.0	32.0	36.0
16	34.96314712691417	36.0	36.0	36.0	32.0	36.0
17	34.943256138299866	36.0	36.0	36.0	32.0	36.0
18	34.946865922661395	36.0	36.0	36.0	32.0	36.0
19	34.92412658857432	36.0	36.0	36.0	32.0	36.0
20	34.8833165406575	36.0	36.0	36.0	32.0	36.0
21	34.86316532966202	36.0	36.0	36.0	32.0	36.0
22	34.865858567213	36.0	36.0	36.0	32.0	36.0
23	34.819596668128014	36.0	36.0	36.0	32.0	36.0
24	34.83261676037236	36.0	36.0	36.0	32.0	36.0
25	34.803476981215276	36.0	36.0	36.0	32.0	36.0
26	34.750730032739305	36.0	36.0	36.0	32.0	36.0
27	34.729162340309344	36.0	36.0	36.0	32.0	36.0
28	34.73242473420142	36.0	36.0	36.0	32.0	36.0
29	34.69341188716347	36.0	36.0	36.0	32.0	36.0
30	34.679159904730405	36.0	36.0	36.0	32.0	36.0
31	34.66278640485475	36.0	36.0	36.0	32.0	36.0
32	34.63233397683893	36.0	36.0	36.0	32.0	36.0
33	34.57870893806194	36.0	36.0	36.0	32.0	36.0
34	34.56047157935757	36.0	36.0	36.0	32.0	36.0
35	34.512627835847105	36.0	36.0	36.0	32.0	36.0
36	34.48732345257415	36.0	36.0	36.0	32.0	36.0
37	34.46012188149755	36.0	36.0	36.0	32.0	36.0
38	34.42965278899426	36.0	36.0	36.0	32.0	36.0
39	34.37838513425167	36.0	36.0	36.0	32.0	36.0
40	34.34669697039618	36.0	36.0	36.0	32.0	36.0
41	34.325313869211975	36.0	36.0	36.0	32.0	36.0
42	34.235640980282064	36.0	36.0	36.0	32.0	36.0
43	34.22049552494418	36.0	36.0	36.0	32.0	36.0
44	34.21507956651823	36.0	36.0	36.0	32.0	36.0
45	34.111346977702915	36.0	36.0	36.0	32.0	36.0
46	34.131613558227	36.0	36.0	36.0	32.0	36.0
47	34.05167401185999	36.0	36.0	36.0	32.0	36.0
48	33.9834598552497	36.0	36.0	36.0	32.0	36.0
49	33.9919843815296	36.0	36.0	36.0	32.0	36.0
50	33.43744664159302	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	24.0
22	58.0
23	222.0
24	558.0
25	1317.0
26	2885.0
27	5483.0
28	9690.0
29	14949.0
30	21576.0
31	31737.0
32	46369.0
33	72649.0
34	150047.0
35	422531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.517525730901184	20.368694198431786	11.997497423063708	25.11628264760332
2	16.042083564616885	20.99101743824569	37.67112312147546	25.295775875661963
3	17.943212408665556	26.040635815921036	30.23945647993847	25.776695295474937
4	12.369167582146416	16.271354606647087	38.198515320452096	33.1609624907544
5	13.050105050499871	38.58692656463714	33.92958091324072	14.433387471622265
6	30.363186069406844	38.111206825826564	17.685387439494626	13.840219665271967
7	28.37910939851455	32.56651053323797	20.951106393779277	18.10327367446821
8	28.566008034846725	32.51164591297035	20.11211354412628	18.810232508056636
9	28.68970980717906	13.826525249262275	20.42207301096523	37.06169193259343
10	17.23966865871386	26.188242050398536	31.000433276674077	25.571656014213527
11	36.86069257609903	22.40014767299661	21.337722502954744	19.401437247949627
12	22.207736936964654	23.9012334284491	30.398717090841963	23.49231254374428
13	29.642918490146158	21.422583200658377	26.241696598649924	22.69280171054554
14	23.16171475012242	19.709217512581688	26.927888917090332	30.201178820205566
15	26.825594601731567	25.803164201604407	23.89174748943087	23.479493707233157
16	24.674465646800034	26.663436319865863	23.44731842759024	25.214779605743864
17	23.013144434958505	26.100689397027566	26.749194336125274	24.13697183188865
18	21.94777093251908	28.20861887291662	27.14375812393764	22.69985207062666
19	25.521021609994595	26.367833949919365	26.190036687510094	21.921107752575946
20	24.85969783438576	25.02518901374436	27.054667210185336	23.06044594168455
21	25.633314617832028	24.318742933616374	25.02634270903036	25.021599739521243
22	25.243237422798558	24.348354445957067	27.015954323921743	23.39245380732263
23	23.81150157287124	26.97198571468859	27.011980484603292	22.204532227836875
24	22.6945963476571	26.68484377683944	26.50127803800016	24.1192818375033
25	24.044676209008564	25.542429066968165	25.819187747243312	24.59370697677996
26	22.785610086886074	24.43103594145381	26.288228975185294	26.49512499647482
27	27.208749625049034	25.299512115082383	25.025573578839687	22.466164681028893
28	23.82726874177992	24.71471679344496	28.084532535488947	23.373481929286168
29	24.476414622703185	25.4617985853132	27.060435686615342	23.001351105368272
30	22.705620547056668	25.886743015656926	27.33796349708115	24.069672940205255
31	22.135182322311696	26.797905914867542	26.071334261417096	24.995577501403663
32	24.063519898679917	26.032493186788397	26.342580841992458	23.56140607253923
33	24.517178522808557	25.983397042950795	25.300537622003276	24.198886812237376
34	23.059035869668325	25.760605664387477	27.135810445300745	24.044548020643454
35	23.104414550917703	26.551143312028426	26.9823689722626	23.362073164791273
36	24.74894308692966	26.00070247224081	25.634596501483138	23.615757939346395
37	24.73535512022787	26.349631202073574	26.584472286957343	22.33054139074121
38	23.574353097415464	25.298614796526607	25.485897997954115	25.641134108103813
39	24.94609679247073	25.31835580475374	26.613314669107375	23.12223273366816
40	24.462570279271173	25.53691696726838	26.235928122219914	23.764584631240528
41	22.883674186196163	26.64510538365496	26.153503003453395	24.317717426695484
42	24.58242640065017	27.665997523400787	25.05659516319661	22.694980912752435
43	24.917382598685816	24.82521516417084	24.46039107706428	25.797011160079066
44	23.426423724077107	26.01505956913327	26.860205460311597	23.698311246478024
45	23.88046691330108	26.972370279783924	26.450387257051	22.696775549863993
46	23.750483911078295	25.683564456955626	26.505123688953496	24.06082794301258
47	24.598706323019297	24.837136682126186	27.30873654983579	23.25542044501873
48	23.177994672491543	28.691504444290615	25.11466449259199	23.01583639062584
49	23.56409802820657	25.853798605823343	26.778549471735747	23.803553894234344
50	21.63242755434546	27.618183263214295	25.99352392379458	24.755865258645667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	30.0
2	39.0
3	86.5
4	134.0
5	134.0
6	134.0
7	155.0
8	176.0
9	234.0
10	292.0
11	407.0
12	522.0
13	920.0
14	1318.0
15	1956.0
16	2594.0
17	3097.5
18	3601.0
19	3987.5
20	4374.0
21	4860.5
22	5347.0
23	5538.5
24	5730.0
25	5381.0
26	5032.0
27	5547.5
28	6063.0
29	7331.5
30	8600.0
31	10782.5
32	12965.0
33	15774.0
34	18583.0
35	22625.5
36	26668.0
37	28928.0
38	31188.0
39	33172.5
40	35157.0
41	36610.5
42	38064.0
43	42378.0
44	46692.0
45	53683.5
46	60675.0
47	70255.5
48	79836.0
49	78884.5
50	77933.0
51	73962.5
52	69992.0
53	67129.5
54	64267.0
55	55454.0
56	46641.0
57	42055.5
58	37470.0
59	33653.0
60	29836.0
61	24535.5
62	19235.0
63	16921.5
64	14608.0
65	11577.5
66	8547.0
67	7059.5
68	5572.0
69	4789.5
70	4007.0
71	3378.5
72	2750.0
73	2251.5
74	1753.0
75	1703.0
76	1653.0
77	1386.5
78	1120.0
79	842.0
80	564.0
81	380.5
82	197.0
83	145.0
84	93.0
85	64.0
86	35.0
87	24.0
88	13.0
89	8.0
90	3.0
91	1.5
92	0.0
93	1.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013587966701790279
2	0.004486592778893017
3	2.563767302224581E-4
4	1.2818836511122904E-4
5	1.2818836511122904E-4
6	7.691301906673743E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	780102.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.2981537050522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.60178759658206	26.27299885069332
2	12.676931838990166	8.695907134443095
3	4.033014195430803	4.149748223086293
4	1.7568339688595127	2.4102464599280182
5	0.9243125587807084	1.5851107106285414
6	0.5639858282001557	1.1606203573848062
7	0.38108900864811673	0.9149454575743352
8	0.28885785256018476	0.7925832820816417
9	0.2379733918874746	0.7345843175402301
>10	1.8336954342607799	13.143603020634275
>50	0.35150784878042074	8.599226400731167
>100	0.3170683144766431	21.619910596755435
>500	0.026578335688124743	6.08722411382437
>1k	0.006363826854903108	3.8332910746944884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCCTAAGGTGTGCGAGAGAACTCTCGTTAAGGAACTCGGCAAAATGACC	4558	0.584282568176982	No Hit
CCTTAGGATACTCTCCTCGACTACCTGTGTCGGTTTGCGGTACGGGTAGT	4187	0.536724684720716	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3027	0.38802618119169036	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2390	0.3063701926158374	No Hit
TCCTTAGCCTGCTGCGTCCCTCCATCGTTCAAACAAATATAACTAGTACA	1541	0.19753827063640397	No Hit
GAATATAACTACCCGTACCGCAAACCGACACAGGTAGTCGAGGAGAGTAT	1404	0.17997646461616557	No Hit
GGCCTATTCACTGCGGCTGACCTTTCGGTCAGCACCCCTTCTCCCGAAGT	1401	0.17959189952083188	No Hit
TTCCAGCACCGGGCAGGCGTCAGCCCCTATACGTCATCTTACGATTTTGC	1309	0.16779856993059883	No Hit
GTTATATTCTAACTAGAAGCTTTTCTTGGCAGTGTGACATCAGTTACTTC	1280	0.1640811073423732	No Hit
ATTCAGACTCGCTTTCGCTACGGCTCCGTCTTATCAACTTAACCTCGCAA	1240	0.15895357273792401	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1140	0.14613473622680112	No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA	1108	0.14203270854324176	No Hit
GGCTAAGGAAGCGCACTGATGGATATGTGCGTCCAAGCAATAAGTCTGAA	1089	0.1395971296061284	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA	1069	0.13703336230390384	No Hit
GAATAGGCCCAAACGACTGTTTATCAAAAACACAGGTTTCTGCAAAATCG	1049	0.13446959500167927	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1034	0.13254676952501082	No Hit
AAGCTAGACCGTAAGGACTAGTGGAGCGCTTAGAAGTGAGAATGCCGGTA	1030	0.1320340160645659	No Hit
GACTAGTGGAGCGCTTAGAAGTGAGAATGCCGGTATGAGTAGCGAAAGAC	975	0.12498365598344832	No Hit
GTCTACGCCTCTCGGCCTCGACTTAGGTCCCGACTAACCCTGGGAGGACG	964	0.12357358396722479	No Hit
AATATAACTAGTACAGGAATCTCAACCTGTTATCCATCGTCTACGCCTCT	961	0.12318901887189111	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	947	0.1213943817603339	No Hit
ATTCAGACTCGCTTTCGCTGCGGCTCCGTCTTATCAACTTAACCTTGCTA	898	0.11511315186988368	No Hit
TTTAAAGAGTGCGTAATAGCTCACTAGTCGAGTGATTCTGCGCCGAAAAT	893	0.11447221004432753	No Hit
CCATTTAAAGAGTGCGTAATAGCTCACTAGTCGAGTGATTCTGCGCCGAA	892	0.11434402167921631	No Hit
TTTCTTGGCAGTGTGACATCAGTTACTTCGCTACTTAATTTCGCTCCCCA	838	0.10742184996320994	No Hit
GCTTAGAAGTGAGAATGCCGGTATGAGTAGCGAAAGACAGGTGAGAATCC	820	0.10511445939120781	No Hit
ACTTAATTTCGCTCCCCATCACAACTTGTCCTTAAAGTTAAGCATTTAAC	803	0.10293525718431693	No Hit
GTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCTCGT	794	0.10178156189831586	No Hit
ATTCTGGGCTTCGCCGAAGCTAACTCATCCTCTTAACCTTCCAGCACCGG	789	0.10114062007275972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.2818836511122904E-4	0.0
13	0.0	0.0	0.0	1.2818836511122904E-4	0.0
14	0.0	0.0	0.0	1.2818836511122904E-4	0.0
15	0.0	0.0	0.0	1.2818836511122904E-4	0.0
16	0.0	0.0	0.0	1.2818836511122904E-4	0.0
17	0.0	0.0	0.0	1.2818836511122904E-4	0.0
18	0.0	0.0	0.0	2.563767302224581E-4	0.0
19	0.0	0.0	0.0	2.563767302224581E-4	0.0
20	0.0	0.0	0.0	2.563767302224581E-4	0.0
21	0.0	0.0	0.0	5.127534604449162E-4	0.0
22	0.0	0.0	0.0	7.691301906673743E-4	0.0
23	0.0	0.0	0.0	0.0014100720162235196	0.0
24	0.0	0.0	0.0	0.003589274223114413	0.0
25	0.0	0.0	0.0	0.004999346239337933	0.0
26	0.0	0.0	0.0	0.006024853160227765	0.0
27	0.0	0.0	0.0	0.008588620462452345	0.0
28	0.0	0.0	0.0	0.012947024876234134	0.0
29	0.0	0.0	0.0	0.02140745697357525	0.0
30	0.0	0.0	0.0	0.043712232502929105	0.0
31	0.0	0.0	0.0	0.07947678636896201	0.0
32	0.0	0.0	0.0	0.13357227644590067	0.0
33	0.0	0.0	0.0	0.18702682469728318	0.0
34	0.0	0.0	0.0	0.25099281888778646	0.0
35	0.0	0.0	0.0	0.3231628684454084	0.0
36	0.0	0.0	0.0	0.42212428631127724	0.0
37	0.0	0.0	0.0	0.5767194546354195	0.0
38	0.0	0.0	0.0	0.7545167170446941	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTG	30	0.0057439045	29.333204	7
GTGTAAT	40	7.0221623E-4	27.505169	1
TAGGATA	680	0.0	26.529295	4
GATACTC	725	0.0	25.186096	7
CGCGAAT	70	1.1314514E-6	25.142746	13
ACTCTCC	725	0.0	24.579203	10
ATACTCT	725	0.0	24.579203	8
TACTCTC	725	0.0	24.579203	9
GTATGAC	55	1.5929584E-4	23.999897	3
AATGACC	845	0.0	23.692202	44
CGCGTGG	75	2.062785E-6	23.466564	10
TGTGCGA	895	0.0	23.351852	10
TCTCCTC	765	0.0	23.294016	12
AAATGAC	870	0.0	23.011395	43
TTGCGGT	785	0.0	22.980793	35
GGTGTGC	910	0.0	22.96693	8
ACTCTCG	930	0.0	22.946136	21
CTCCTCG	770	0.0	22.857042	13
GTGTGCG	925	0.0	22.83233	9
AGAGTTA	155	0.0	22.709578	40
>>END_MODULE