FastQCFastQC Report
Thu 2 Feb 2017
SRR4062335_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062335_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869294
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG42250.22602116093027635No Hit
GTATCAACGCAGAGTACTTTTTTTT38470.20579962274527175No Hit
TCCATGTACTCTGCGTTGATACCAC35790.191462659164369No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27290.14599094631449092No Hit
TATCAACGCAGAGTACTTTTTTTTT26980.14433256619878948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA801.996852E-613.0643429
CGCGTAA801.9974523E-613.06399210
CGCATCG2250.012.66743613
CGTCGTA1600.012.47017410
CGTTATT1405.2750693E-1112.207832
GCGTTAT1405.2750693E-1112.2065231
CGGTCCA2550.011.92293110
ACCGTCG1701.8189894E-1211.7366348
GTCCTAA3000.011.7092211
TCGGCGT1302.6138878E-911.69301713
GCGTAAC1001.9299096E-611.4000811
CGGCGTC1604.5474735E-1111.28163114
GCGGTCG1354.7184585E-911.2608489
CCGTCGT1701.0913936E-1111.1780469
CGCCGGT2300.011.1534497
CGTAAGA600.00589957511.07747652
TAGACAG2350.010.9149715
CGACCAT1752.0008883E-1110.85838310
TAGATCG700.001491557210.8572215
GTCTTAA1502.1555024E-910.7598251
Produced by FastQC (version 0.11.5)