##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062335_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1869294
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151929552012685	32.0	32.0	32.0	32.0	32.0
2	30.794683447333593	32.0	32.0	32.0	32.0	32.0
3	30.79394359581746	32.0	32.0	32.0	32.0	32.0
4	30.865350768792926	32.0	32.0	32.0	32.0	32.0
5	30.65658639036984	32.0	32.0	32.0	32.0	32.0
6	34.30703570438893	36.0	36.0	36.0	32.0	36.0
7	34.18745472889765	36.0	36.0	36.0	32.0	36.0
8	34.18002465101798	36.0	36.0	36.0	32.0	36.0
9	34.350712621984556	36.0	36.0	36.0	32.0	36.0
10	33.997961797341674	36.0	36.0	36.0	32.0	36.0
11	34.33839192764755	36.0	36.0	36.0	32.0	36.0
12	34.11510762886951	36.0	36.0	36.0	32.0	36.0
13	34.23367003799295	36.0	36.0	36.0	32.0	36.0
14	34.137735958067594	36.0	36.0	36.0	32.0	36.0
15	34.08054431245165	36.0	36.0	36.0	32.0	36.0
16	34.078646804622494	36.0	36.0	36.0	32.0	36.0
17	34.01177449882148	36.0	36.0	36.0	32.0	36.0
18	34.0171834928053	36.0	36.0	36.0	32.0	36.0
19	34.006259582494785	36.0	36.0	36.0	32.0	36.0
20	33.98438715365266	36.0	36.0	36.0	32.0	36.0
21	33.96704103260375	36.0	36.0	36.0	32.0	36.0
22	33.92393224393808	36.0	36.0	36.0	32.0	36.0
23	33.8785493346686	36.0	36.0	36.0	32.0	36.0
24	33.85472804170986	36.0	36.0	36.0	32.0	36.0
25	33.376148428230124	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	76.0
5	190.0
6	323.0
7	90.0
8	286.0
9	367.0
10	204.0
11	84.0
12	146.0
13	94.0
14	324.0
15	361.0
16	640.0
17	833.0
18	1151.0
19	1713.0
20	2600.0
21	3849.0
22	5735.0
23	8309.0
24	11615.0
25	16243.0
26	22501.0
27	29306.0
28	39810.0
29	52979.0
30	67985.0
31	93100.0
32	133066.0
33	189450.0
34	415503.0
35	770352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.866195148571	17.715740928983383	11.540869062777533	25.877194859668084
2	16.1997084353141	20.20404178202196	37.87419920020329	25.722050582460643
3	18.687546878293517	24.54732019332314	28.558871646831108	28.206261281552237
4	12.45046895983478	15.968390020491913	36.29369245009443	35.28744856957887
5	14.219308822026248	36.947897551491565	34.056438833847885	14.776354792634299
6	33.504318700739574	35.58986205205991	17.464110735147877	13.44170851205265
7	29.591097092357817	30.698393018318242	21.530545928369452	18.17996396095449
8	27.624454644172054	33.455558720660015	19.98674225792841	18.933244377239518
9	27.26342434350212	14.72828947755947	18.99652306062769	39.011763118310725
10	15.594473837280923	27.460865988304516	32.33085847911621	24.61380169529835
11	36.3821194819663	21.442106841772627	22.90119224877615	19.274581427484925
12	24.232113356888643	23.997113796140155	29.629389568954988	22.14138327801621
13	29.3656171014131	20.330075569340988	25.42437790133726	24.87992942790865
14	23.158796287865073	20.022357224961677	25.829343777417595	30.989502709755655
15	24.75012184606156	27.74389296827718	23.00973161731232	24.496253568348948
16	25.056462181719052	26.101996648419888	24.69281199070898	24.148729179152078
17	23.48589661391254	26.20924999584887	26.196876960068305	24.107976430170282
18	24.168384356948184	25.26729546272648	27.09813546684036	23.466184713484974
19	24.943754017570175	25.77587315191772	25.828797943004073	23.451574887508034
20	24.997817301434686	24.987318923059206	26.13887319190875	23.875990583597357
21	25.753531496756825	24.887631150557386	24.888220380610473	24.470616972075316
22	24.993329625783968	25.398749077130955	25.284200803445234	24.32372049363984
23	23.86971622166757	25.283277430539375	25.74694650742808	25.10005984036498
24	24.12847693187881	25.816667845196843	25.71740346230739	24.33745176061695
25	24.433478749025088	25.295688096197193	25.721382534689784	24.549450620087935
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	301.0
1	301.0
2	704.5
3	1108.0
4	1108.0
5	1108.0
6	3074.5
7	5041.0
8	5041.0
9	5041.0
10	5066.0
11	5091.0
12	5091.0
13	5091.0
14	6046.5
15	7002.0
16	7002.0
17	7002.0
18	11301.0
19	15600.0
20	15600.0
21	15600.0
22	24141.0
23	32682.0
24	32682.0
25	32682.0
26	47676.0
27	62670.0
28	62670.0
29	62670.0
30	77817.5
31	92965.0
32	92965.0
33	92965.0
34	110818.5
35	128672.0
36	128672.0
37	128672.0
38	144344.5
39	160017.0
40	160017.0
41	160017.0
42	180196.5
43	200376.0
44	200376.0
45	200376.0
46	227067.0
47	253758.0
48	253758.0
49	253758.0
50	249058.5
51	244359.0
52	244359.0
53	244359.0
54	225253.5
55	206148.0
56	206148.0
57	206148.0
58	186708.5
59	167269.0
60	167269.0
61	167269.0
62	145575.5
63	123882.0
64	123882.0
65	123882.0
66	101380.5
67	78879.0
68	78879.0
69	78879.0
70	59933.0
71	40987.0
72	40987.0
73	40987.0
74	31196.5
75	21406.0
76	21406.0
77	21406.0
78	16777.5
79	12149.0
80	12149.0
81	12149.0
82	8548.5
83	4948.0
84	4948.0
85	4948.0
86	3603.0
87	2258.0
88	2258.0
89	2258.0
90	1525.5
91	793.0
92	793.0
93	793.0
94	481.5
95	170.0
96	170.0
97	170.0
98	466.5
99	763.0
100	763.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008238404445742618
2	0.003691233160754809
3	0.004921644214339745
4	0.014122979049844487
5	0.036751843209254405
6	0.0485744885502227
7	0.07061489524922243
8	0.09126440249634354
9	0.09880735721614685
10	0.12025930645473638
11	0.11951036059603252
12	0.132777401521644
13	0.12127573297726307
14	0.12496696613801789
15	0.1168355539578044
16	0.11314432079704959
17	0.12469948547419508
18	0.13085154074211977
19	0.13341935511481876
20	0.12512745453631158
21	0.13111902140594256
22	0.15150104798924086
23	0.1423532092865007
24	0.137057092142809
25	0.13170747886635276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1869294.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.75418749502336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.147844165632	46.28873619263824
2	12.345039428423853	14.259554435652944
3	3.414041836148543	5.915256369623304
4	1.3996785475579103	3.2334918907368606
5	0.7043242712715525	2.0338838010156466
6	0.41599300330609534	1.4415202745734859
7	0.27879545417351714	1.1271123453168248
8	0.20361662971465014	0.9407770407715719
9	0.14432741154474124	0.7501961148323746
>10	0.7959389474140367	8.390163897087307
>50	0.07097146590971448	2.878161325222459
>100	0.07170090098244199	8.515187623862964
>500	0.0057726765192986805	2.2710199177792516
>1k	0.00195526140169794	1.9549387708868586
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	4225	0.22602116093027635	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3847	0.20579962274527175	No Hit
TCCATGTACTCTGCGTTGATACCAC	3579	0.191462659164369	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2729	0.14599094631449092	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2698	0.14433256619878948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.069922655291249E-4
2	0.0	0.0	0.0	0.0	1.069922655291249E-4
3	0.0	0.0	0.0	0.0	1.069922655291249E-4
4	0.0	0.0	0.0	0.0	1.069922655291249E-4
5	0.0	0.0	0.0	5.349613276456245E-5	1.069922655291249E-4
6	0.0	0.0	0.0	5.349613276456245E-5	1.069922655291249E-4
7	0.0	0.0	0.0	5.349613276456245E-5	1.069922655291249E-4
8	0.0	0.0	0.0	5.349613276456245E-5	1.069922655291249E-4
9	0.0	0.0	0.0	1.6048839829368734E-4	1.069922655291249E-4
10	0.0	0.0	0.0	1.6048839829368734E-4	1.069922655291249E-4
11	0.0	0.0	0.0	2.139845310582498E-4	1.069922655291249E-4
12	0.0	0.0	0.0	2.139845310582498E-4	1.6048839829368734E-4
13	0.0	0.0	0.0	2.139845310582498E-4	2.6748066382281226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	80	1.996852E-6	13.064342	9
CGCGTAA	80	1.9974523E-6	13.063992	10
CGCATCG	225	0.0	12.667436	13
CGTCGTA	160	0.0	12.470174	10
CGTTATT	140	5.2750693E-11	12.20783	2
GCGTTAT	140	5.2750693E-11	12.206523	1
CGGTCCA	255	0.0	11.922931	10
ACCGTCG	170	1.8189894E-12	11.736634	8
GTCCTAA	300	0.0	11.709221	1
TCGGCGT	130	2.6138878E-9	11.693017	13
GCGTAAC	100	1.9299096E-6	11.40008	11
CGGCGTC	160	4.5474735E-11	11.281631	14
GCGGTCG	135	4.7184585E-9	11.260848	9
CCGTCGT	170	1.0913936E-11	11.178046	9
CGCCGGT	230	0.0	11.153449	7
CGTAAGA	60	0.005899575	11.0774765	2
TAGACAG	235	0.0	10.914971	5
CGACCAT	175	2.0008883E-11	10.858383	10
TAGATCG	70	0.0014915572	10.857221	5
GTCTTAA	150	2.1555024E-9	10.759825	1
>>END_MODULE