FastQCFastQC Report
Thu 2 Feb 2017
SRR4062335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869294
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAT1801.4551915E-1118.3399681
TAGCGTG600.00741412518.3330975
TACGTAC600.00741412518.33309727
TAGGACG7350.017.660114
CGCAATA4050.016.8392936
GTATTAG4500.016.6282371
GTCCTAA5350.016.454551
GTCCTAC9200.016.2667541
CGTCGTA3250.016.24594710
ACCGTCG3400.016.1762648
CGTATAC1105.6232082E-516.0002233
CAATACG4150.015.90341138
ATACCGT3350.015.7609936
CCGTCGT3500.015.7140869
CGAATGC4200.015.71408543
AATACGA4200.015.71408539
CTAGCGG4350.015.6779629
ATACGAT850.002981009715.5292136
TCTAGCG4400.015.49980328
GTATAGT2001.2714736E-915.4055721
Produced by FastQC (version 0.11.5)