##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1869294
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24994891119321	32.0	32.0	32.0	32.0	32.0
2	31.38467945652209	32.0	32.0	32.0	32.0	32.0
3	31.486071746873417	32.0	32.0	32.0	32.0	32.0
4	31.595437635813308	32.0	32.0	32.0	32.0	32.0
5	31.522308957285478	32.0	32.0	32.0	32.0	32.0
6	35.11545642365513	36.0	36.0	36.0	36.0	36.0
7	35.13272604523419	36.0	36.0	36.0	36.0	36.0
8	35.07693546333535	36.0	36.0	36.0	36.0	36.0
9	35.220573114769536	36.0	36.0	36.0	36.0	36.0
10	35.0745388365875	36.0	36.0	36.0	36.0	36.0
11	35.21624581259021	36.0	36.0	36.0	36.0	36.0
12	35.130012721380375	36.0	36.0	36.0	36.0	36.0
13	35.170854343939475	36.0	36.0	36.0	36.0	36.0
14	35.13470112245586	36.0	36.0	36.0	36.0	36.0
15	35.09171804970219	36.0	36.0	36.0	36.0	36.0
16	35.11331122873128	36.0	36.0	36.0	36.0	36.0
17	35.08551089341751	36.0	36.0	36.0	36.0	36.0
18	35.091184693258526	36.0	36.0	36.0	36.0	36.0
19	35.07949311344283	36.0	36.0	36.0	36.0	36.0
20	35.067113573359784	36.0	36.0	36.0	36.0	36.0
21	35.06021096734917	36.0	36.0	36.0	36.0	36.0
22	35.03937208379206	36.0	36.0	36.0	36.0	36.0
23	34.99576310628505	36.0	36.0	36.0	36.0	36.0
24	34.98665592464321	36.0	36.0	36.0	36.0	36.0
25	34.95400135024239	36.0	36.0	36.0	32.0	36.0
26	34.91006497640286	36.0	36.0	36.0	32.0	36.0
27	34.88361327859609	36.0	36.0	36.0	32.0	36.0
28	34.87203778538849	36.0	36.0	36.0	32.0	36.0
29	34.842119003217256	36.0	36.0	36.0	32.0	36.0
30	34.82961695699018	36.0	36.0	36.0	32.0	36.0
31	34.82218259941989	36.0	36.0	36.0	32.0	36.0
32	34.783732788956684	36.0	36.0	36.0	32.0	36.0
33	34.76030576249643	36.0	36.0	36.0	32.0	36.0
34	34.74908334376508	36.0	36.0	36.0	32.0	36.0
35	34.71273165162891	36.0	36.0	36.0	32.0	36.0
36	34.68077466679934	36.0	36.0	36.0	32.0	36.0
37	34.664149138658765	36.0	36.0	36.0	32.0	36.0
38	34.633659017789604	36.0	36.0	36.0	32.0	36.0
39	34.59666965175087	36.0	36.0	36.0	32.0	36.0
40	34.584186329170265	36.0	36.0	36.0	32.0	36.0
41	34.56079514511896	36.0	36.0	36.0	32.0	36.0
42	34.520539305213624	36.0	36.0	36.0	32.0	36.0
43	34.51174454098713	36.0	36.0	36.0	32.0	36.0
44	34.48944307316024	36.0	36.0	36.0	32.0	36.0
45	34.430660987517214	36.0	36.0	36.0	32.0	36.0
46	34.438688082238535	36.0	36.0	36.0	32.0	36.0
47	34.402807156070686	36.0	36.0	36.0	32.0	36.0
48	34.36732317120795	36.0	36.0	36.0	32.0	36.0
49	34.35273424084173	36.0	36.0	36.0	32.0	36.0
50	33.83931313105376	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	43.0
22	149.0
23	450.0
24	1229.0
25	2865.0
26	6031.0
27	11537.0
28	19280.0
29	29452.0
30	42635.0
31	61276.0
32	90727.0
33	147865.0
34	325178.0
35	1130566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.329511327174245	18.239476597390798	12.041727104479879	26.389284970955075
2	15.593752056335708	20.715880341724876	37.490336893992854	26.200030707946564
3	18.0705211947689	24.844619484914528	28.987921565606474	28.0969377547101
4	12.292735587984964	16.486304165590056	36.36640844041939	34.85455180600559
5	14.030751716958381	37.117970741895064	33.94458014630122	14.906697394845327
6	33.633537083142976	35.81923793362813	17.222351207894352	13.32487377533454
7	29.498746317457993	30.83615035203149	21.541834265682265	18.123269064828254
8	27.731821603140865	33.61072876215767	19.815994603307242	18.841455031394222
9	27.30132338733233	14.540462869939134	18.79570575843072	39.36250798429782
10	15.482208791126489	27.52680958693496	32.4012167160436	24.589764905894953
11	36.55069775005965	21.345652422786358	22.869222283921093	19.234427543232897
12	24.18084046704264	24.005373151574872	29.55976962425386	22.25401675712863
13	29.545860629735078	20.14338033503558	25.386536307290346	24.924222727938997
14	23.160134253894785	19.894248844751015	25.727627649797196	31.217989251557004
15	24.85906443823176	27.798088476184056	22.815030701430594	24.52781638415359
16	25.227866777510656	25.97895248152511	24.653746280681368	24.139434460282867
17	23.62148490285637	26.150354090902766	26.09252477138428	24.135636234856584
18	24.243698423041	25.200048788473083	27.135806352558774	23.420446435927147
19	25.095142872121773	25.66214838329337	25.740520217793456	23.502188526791397
20	25.11622034843101	24.921815401964594	26.014206433016955	23.94775781658744
21	25.806052980430046	24.806584731989727	24.903466228426346	24.483896059153885
22	24.95958367169637	25.350372921541503	25.378351398977365	24.311692007784757
23	23.971242618871084	25.147676074496573	25.76603787312215	25.11504343351019
24	24.23225025062938	25.625396540084118	25.669370361216586	24.47298284806991
25	24.60367390041374	24.981891559059196	25.769461625619083	24.64497291490798
26	23.866336702519774	25.54215655750246	26.204064208198393	24.387442531779378
27	24.707830870906342	25.749935537160017	25.407292806802996	24.13494078513064
28	24.113167859095466	25.178864319898313	25.923423495715497	24.784544325290724
29	24.14237674758492	25.567888197362215	25.914061672481697	24.375673382571176
30	24.335604779130517	25.347805107168803	26.14569992735225	24.17089018634843
31	24.449926014848387	25.09883410528253	25.54745267464615	24.903787205222937
32	24.024096798042468	25.662415863957193	25.501285512070332	24.812201825930003
33	23.892924280503763	25.346200223185868	25.94075624273121	24.820119253579158
34	24.303721084002838	25.441476835639556	26.31544315661421	23.939358923743402
35	24.972743720356455	25.327904545780385	25.739450295138166	23.959901438724994
36	24.123385620453497	25.60223271459706	25.750577490753194	24.523804174196247
37	24.841517706684986	25.344755827601222	25.420131878666492	24.3935945870473
38	24.139487956415632	25.558954343190532	25.66947735348212	24.63208034691172
39	24.42617373190092	25.327904545780385	25.482080400407852	24.76384132191084
40	24.947975010886463	25.209945573034524	25.85163168554545	23.99044773053356
41	24.053840647859566	25.34593274252204	26.189085291024313	24.41114131859408
42	24.80016519605798	25.711525313835065	25.729553510576718	23.758755979530243
43	24.093213801574283	25.078987040026878	25.903950903389195	24.923848255009645
44	23.874896083762103	25.405366946023474	26.030522753510148	24.689214216704276
45	24.072403805928868	25.496952325316403	25.911868331038352	24.51877553771638
46	24.057478384887556	25.095035879856248	26.0453946784187	24.8020910568375
47	24.38722854724832	24.973546162347922	26.276123499032256	24.363101791371502
48	24.35357947973941	26.015383347937775	25.370701451992034	24.26033572033078
49	23.792244558640856	25.386482811157578	26.128955637796942	24.69231699240462
50	23.509784977643967	26.19234855512295	25.521988515450218	24.775877951782867
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	59.5
2	49.0
3	187.0
4	325.0
5	341.0
6	357.0
7	342.5
8	328.0
9	421.0
10	514.0
11	774.0
12	1034.0
13	1773.0
14	2512.0
15	3294.0
16	4076.0
17	4830.0
18	5584.0
19	6264.0
20	6944.0
21	8061.5
22	9179.0
23	10199.0
24	11219.0
25	13321.0
26	15423.0
27	19468.0
28	23513.0
29	27024.5
30	30536.0
31	33933.0
32	37330.0
33	43186.0
34	49042.0
35	56222.5
36	63403.0
37	72045.0
38	80687.0
39	84709.5
40	88732.0
41	95495.5
42	102259.0
43	104524.0
44	106789.0
45	116579.0
46	126369.0
47	132219.5
48	138070.0
49	138817.0
50	139564.0
51	134594.5
52	129625.0
53	126813.0
54	124001.0
55	122624.0
56	121247.0
57	117061.0
58	112875.0
59	102983.5
60	93092.0
61	82164.5
62	71237.0
63	61746.0
64	52255.0
65	44748.0
66	37241.0
67	32075.5
68	26910.0
69	23961.0
70	21012.0
71	16562.5
72	12113.0
73	10668.5
74	9224.0
75	7052.5
76	4881.0
77	4309.0
78	3737.0
79	3148.0
80	2559.0
81	2015.5
82	1472.0
83	1199.5
84	927.0
85	759.0
86	591.0
87	426.0
88	261.0
89	171.5
90	82.0
91	54.0
92	26.0
93	18.5
94	11.0
95	6.5
96	2.0
97	3.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015674366900016796
2	0.0037982254262839336
3	6.419535931747493E-4
4	1.6048839829368734E-4
5	0.0
6	4.2796906211649955E-4
7	5.3496132764562443E-5
8	5.3496132764562443E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1869294.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.02249895897036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.61927368149559	49.195895443020106
2	12.203643827893723	14.893936855665796
3	3.3675059445448374	6.164808839859415
4	1.3185726298827714	3.218503877373929
5	0.6812900022172135	2.0787009225528412
6	0.37670769374503527	1.3792586909635525
7	0.26561448564859935	1.1345921771785368
8	0.19517338046755156	0.9527973925119911
9	0.1439212495073182	0.7904190868410633
>10	0.7007893854973933	7.790663045594466
>50	0.06305364029831831	2.707644062879536
>100	0.05942214391991134	7.369349548053606
>500	0.004237418847744923	1.7372124390082098
>1k	7.945160339521728E-4	0.5862176184968785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.349613276456245E-5	5.349613276456245E-5
6	0.0	0.0	0.0	5.349613276456245E-5	1.6048839829368734E-4
7	0.0	0.0	0.0	5.349613276456245E-5	1.6048839829368734E-4
8	0.0	0.0	0.0	5.349613276456245E-5	1.6048839829368734E-4
9	0.0	0.0	0.0	5.349613276456245E-5	1.6048839829368734E-4
10	5.349613276456245E-5	0.0	0.0	5.349613276456245E-5	1.6048839829368734E-4
11	5.349613276456245E-5	0.0	0.0	1.069922655291249E-4	1.6048839829368734E-4
12	5.349613276456245E-5	0.0	0.0	1.069922655291249E-4	1.6048839829368734E-4
13	5.349613276456245E-5	0.0	0.0	1.069922655291249E-4	1.6048839829368734E-4
14	5.349613276456245E-5	0.0	0.0	1.069922655291249E-4	1.6048839829368734E-4
15	5.349613276456245E-5	0.0	0.0	1.6048839829368734E-4	3.209767965873747E-4
16	5.349613276456245E-5	0.0	0.0	3.209767965873747E-4	3.209767965873747E-4
17	5.349613276456245E-5	0.0	0.0	3.209767965873747E-4	3.209767965873747E-4
18	5.349613276456245E-5	0.0	0.0	3.209767965873747E-4	3.7447292935193714E-4
19	5.349613276456245E-5	0.0	0.0	3.7447292935193714E-4	3.7447292935193714E-4
20	5.349613276456245E-5	0.0	0.0	5.88457460410187E-4	4.279690621164996E-4
21	5.349613276456245E-5	0.0	0.0	7.489458587038743E-4	4.279690621164996E-4
22	5.349613276456245E-5	0.0	0.0	0.001176914920820374	4.8146519488106207E-4
23	5.349613276456245E-5	0.0	0.0	0.002246837576111623	4.8146519488106207E-4
24	1.069922655291249E-4	0.0	0.0	0.003905217691813059	4.8146519488106207E-4
25	1.069922655291249E-4	0.0	0.0	0.00502863647986887	4.8146519488106207E-4
26	1.069922655291249E-4	0.0	0.0	0.006045063002395557	5.349613276456245E-4
27	1.069922655291249E-4	0.0	0.0	0.008559381242329992	5.349613276456245E-4
28	1.069922655291249E-4	0.0	0.0	0.014443955846431862	5.349613276456245E-4
29	1.069922655291249E-4	0.0	0.0	0.023270817752584664	5.349613276456245E-4
30	1.069922655291249E-4	0.0	0.0	0.042475929415062584	5.349613276456245E-4
31	1.6048839829368734E-4	0.0	0.0	0.0816350985987223	5.88457460410187E-4
32	1.6048839829368734E-4	0.0	0.0	0.12839071863494989	5.88457460410187E-4
33	1.6048839829368734E-4	0.0	0.0	0.17589528452988135	5.88457460410187E-4
34	1.6048839829368734E-4	0.0	0.0	0.23211972006543646	6.419535931747493E-4
35	1.6048839829368734E-4	0.0	0.0	0.2932123036825668	6.419535931747493E-4
36	1.6048839829368734E-4	0.0	0.0	0.3848511791082623	6.419535931747493E-4
37	1.6048839829368734E-4	0.0	0.0	0.524636574022064	6.419535931747493E-4
38	1.6048839829368734E-4	0.0	0.0	0.7064699292888117	6.419535931747493E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATAT	180	1.4551915E-11	18.339968	1
TAGCGTG	60	0.007414125	18.333097	5
TACGTAC	60	0.007414125	18.333097	27
TAGGACG	735	0.0	17.66011	4
CGCAATA	405	0.0	16.83929	36
GTATTAG	450	0.0	16.628237	1
GTCCTAA	535	0.0	16.45455	1
GTCCTAC	920	0.0	16.266754	1
CGTCGTA	325	0.0	16.245947	10
ACCGTCG	340	0.0	16.176264	8
CGTATAC	110	5.6232082E-5	16.000223	3
CAATACG	415	0.0	15.903411	38
ATACCGT	335	0.0	15.760993	6
CCGTCGT	350	0.0	15.714086	9
CGAATGC	420	0.0	15.714085	43
AATACGA	420	0.0	15.714085	39
CTAGCGG	435	0.0	15.67796	29
ATACGAT	85	0.0029810097	15.529213	6
TCTAGCG	440	0.0	15.499803	28
GTATAGT	200	1.2714736E-9	15.405572	1
>>END_MODULE