FastQCFastQC Report
Thu 2 Feb 2017
SRR4062334_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062334_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157111
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45060.142725422071001No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG43400.1374674504634142No Hit
GTACATGGAAGCAGTGGTATCAACG43270.13705568160257908No Hit
GAATAGGACCGCGGTTCTATTTTGT40640.12872528080260717No Hit
GTCTTGCGCCGGTCCAAGAATTTCA40490.12825016288625898No Hit
GTATCTGATCGTCTTCGAACCTCCG38270.12121841772430554No Hit
CTATTGGAGCTGGAATTACCGCGGC38210.12102837055776626No Hit
TCCATGTACTCTGCGTTGATACCAC38040.12048990358590496No Hit
GCGCAAGACGGACCAGAGCGAAAGC37510.11881115361480797No Hit
GATTAAGAGGGACGGCCGGGGGCAT37300.11814598853192049No Hit
ATCAGATACCGTCGTAGTTCCGACC36140.11447174331216102No Hit
GAATAACGCCGCCGCATCGCCAGTC35280.11174773392509797No Hit
GAACTACGACGGTATCTGATCGTCT34940.11067079998137538No Hit
GGGTAGGCACACGCTGAGCCAGTCA34820.11029070564829682No Hit
GTCCTATTCCATTATTCCTAGCTGC34610.10962554056540932No Hit
TCGTAGTTCCGACCATAAACGATGC32540.1030689133198041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT400.005289213314.2465394
CCGTCGT7000.012.351319
CGTCGTA7000.012.21558110
ATTTGCG4450.011.95746316
CCGACCA6000.011.876269
CGCATCG6450.011.78419513
CGAACGA2750.011.74781916
AGGCCCG8950.011.57105910
ACCGTCG7650.011.5498778
TCGCGTA3300.011.5163739
CGAGGTT3500.011.3972314
TTGCGCG4850.011.36329118
GTCGTAG7200.011.34806511
TTTGCGC4800.011.28352117
ATCGCCA6850.011.23583716
CGCAAGA7400.011.1631972
GTCCTAA3850.011.0967991
GAGGTTA3600.011.0825755
GCGTAAC3700.011.04136111
CGCGTAA3450.011.0156610
Produced by FastQC (version 0.11.5)