##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062334_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3157111
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209636594975596	32.0	32.0	32.0	32.0	32.0
2	30.81150076763218	32.0	32.0	32.0	32.0	32.0
3	30.799250960767612	32.0	32.0	32.0	32.0	32.0
4	30.888174980227177	32.0	32.0	32.0	32.0	32.0
5	30.633854495454862	32.0	32.0	32.0	32.0	32.0
6	34.31976100935317	36.0	36.0	36.0	32.0	36.0
7	34.20403020356269	36.0	36.0	36.0	32.0	36.0
8	34.20189217293912	36.0	36.0	36.0	32.0	36.0
9	34.39417049321357	36.0	36.0	36.0	32.0	36.0
10	34.012503519831895	36.0	36.0	36.0	32.0	36.0
11	34.356335586553655	36.0	36.0	36.0	32.0	36.0
12	34.105759981198	36.0	36.0	36.0	32.0	36.0
13	34.25224326924204	36.0	36.0	36.0	32.0	36.0
14	34.148270998390615	36.0	36.0	36.0	32.0	36.0
15	34.07771567106763	36.0	36.0	36.0	32.0	36.0
16	34.07960759061053	36.0	36.0	36.0	32.0	36.0
17	33.985508586806105	36.0	36.0	36.0	32.0	36.0
18	34.001304673798295	36.0	36.0	36.0	32.0	36.0
19	33.991455162647114	36.0	36.0	36.0	32.0	36.0
20	33.992575807439145	36.0	36.0	36.0	32.0	36.0
21	33.96214038720843	36.0	36.0	36.0	32.0	36.0
22	33.910864711440304	36.0	36.0	36.0	32.0	36.0
23	33.868933971596185	36.0	36.0	36.0	32.0	36.0
24	33.84395448877154	36.0	36.0	36.0	32.0	36.0
25	33.378484950323255	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	25.0
4	115.0
5	295.0
6	554.0
7	147.0
8	493.0
9	572.0
10	377.0
11	136.0
12	231.0
13	183.0
14	429.0
15	586.0
16	886.0
17	1275.0
18	1775.0
19	2507.0
20	3895.0
21	5693.0
22	8832.0
23	13111.0
24	18995.0
25	26962.0
26	37815.0
27	48991.0
28	67144.0
29	89658.0
30	116020.0
31	159666.0
32	229147.0
33	329790.0
34	722902.0
35	1267903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.11632386002047	17.192673796808382	11.677177728438597	26.01382461473255
2	17.2128548581391	19.390111057931243	36.63803084002824	26.759003243901414
3	19.209435409202335	23.23691881966158	27.629690471114763	29.923955300021316
4	12.936661630735632	15.314056139912926	34.89520057504018	36.854081654311265
5	15.58250250713325	35.68145703652889	32.574291150651376	16.161749305686477
6	35.081520447451396	34.19466670891892	16.7687481184542	13.955064725175479
7	31.395180037033725	29.634366436109218	20.250735191105314	18.719718335751743
8	28.82674557728754	31.762211796901106	19.200605144872725	20.210437480938634
9	27.486540240092754	14.768117371072128	18.540527978323613	39.2048144105115
10	16.89041167135806	25.74595058117041	29.995858296662664	27.36777945080887
11	37.65377011498627	20.908549475683152	21.85075316039408	19.58692724893649
12	25.633232176885073	23.126660390907084	27.6270016670515	23.61310576515634
13	29.510565356792878	18.973655584908173	25.057734450071834	26.458044608227123
14	24.248242450075498	19.709233198577532	24.041399113447003	32.00112523789996
15	25.826070659686383	26.729968704147485	21.656577726067265	25.787382910098867
16	26.69956543688434	25.174662408134168	23.159480604481605	24.966291550499886
17	24.64118489538735	25.323865993309557	24.759352217270568	25.27559689403252
18	25.649323978657335	24.057504552110686	25.732801807079316	24.560369662152667
19	26.106207320902406	24.614337956604892	24.663721220848174	24.615733501644524
20	26.259662449344884	23.714871244512693	24.351338614341177	25.674127691801246
21	27.458239730130984	23.646396994294527	23.58591370522151	25.309449570352978
22	26.27436089238096	23.921719031839118	24.09197642599688	25.71194364978305
23	24.64356165243895	23.69194921477145	24.887293193755475	26.777195939034126
24	25.150707403392165	24.674749222753885	24.461224601132074	25.713318772721884
25	25.400751247030108	24.02268267440898	24.51772040327433	26.058845675286584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	287.0
1	287.0
2	753.0
3	1219.0
4	1219.0
5	1219.0
6	2931.0
7	4643.0
8	4643.0
9	4643.0
10	4776.0
11	4909.0
12	4909.0
13	4909.0
14	5763.5
15	6618.0
16	6618.0
17	6618.0
18	10673.0
19	14728.0
20	14728.0
21	14728.0
22	25851.0
23	36974.0
24	36974.0
25	36974.0
26	57709.5
27	78445.0
28	78445.0
29	78445.0
30	98469.0
31	118493.0
32	118493.0
33	118493.0
34	151605.5
35	184718.0
36	184718.0
37	184718.0
38	210902.5
39	237087.0
40	237087.0
41	237087.0
42	274711.5
43	312336.0
44	312336.0
45	312336.0
46	362629.0
47	412922.0
48	412922.0
49	412922.0
50	419677.5
51	426433.0
52	426433.0
53	426433.0
54	397434.5
55	368436.0
56	368436.0
57	368436.0
58	343979.5
59	319523.0
60	319523.0
61	319523.0
62	286747.0
63	253971.0
64	253971.0
65	253971.0
66	212791.0
67	171611.0
68	171611.0
69	171611.0
70	130904.0
71	90197.0
72	90197.0
73	90197.0
74	71450.0
75	52703.0
76	52703.0
77	52703.0
78	44032.0
79	35361.0
80	35361.0
81	35361.0
82	24974.0
83	14587.0
84	14587.0
85	14587.0
86	10712.0
87	6837.0
88	6837.0
89	6837.0
90	4654.0
91	2471.0
92	2471.0
93	2471.0
94	1455.0
95	439.0
96	439.0
97	439.0
98	801.0
99	1163.0
100	1163.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008805518716320078
2	0.003484198053220175
3	0.00481452821899515
4	0.014633631823524736
5	0.0353487729763065
6	0.046276485052315235
7	0.06806856014881961
8	0.0894805409122454
9	0.0975575454901649
10	0.12096502150225318
11	0.12242204977905434
12	0.13426832316000292
13	0.12292884222315908
14	0.1266981110261882
15	0.11586542253344909
16	0.11399662539581283
17	0.12527275727714357
18	0.13233617696685357
19	0.13369818166038508
20	0.12432252144444714
21	0.13220947885582734
22	0.1528295964253395
23	0.14133174284971292
24	0.1366122382139874
25	0.13366650713262854
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3157111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.15178581522214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.63812659227197	33.070720239896
2	13.168937862538558	11.365263721164661
3	4.098788822943119	5.306101721684038
4	1.8273148086369448	3.1540758895714034
5	0.9790331709487908	2.1123514849389995
6	0.6199740729297988	1.6051793043634552
7	0.41944388370962576	1.2669826841939904
8	0.3082352495606779	1.0640721175795131
9	0.25281054846155443	0.9818303975137652
>10	1.4353504139946234	11.528300496924253
>50	0.11655208979592503	3.4926417276418937
>100	0.10311636493633923	9.459264088702806
>500	0.019610008740161146	5.9175850476168215
>1k	0.012706110531973671	9.675631078208397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4506	0.142725422071001	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	4340	0.1374674504634142	No Hit
GTACATGGAAGCAGTGGTATCAACG	4327	0.13705568160257908	No Hit
GAATAGGACCGCGGTTCTATTTTGT	4064	0.12872528080260717	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	4049	0.12825016288625898	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3827	0.12121841772430554	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3821	0.12102837055776626	No Hit
TCCATGTACTCTGCGTTGATACCAC	3804	0.12048990358590496	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3751	0.11881115361480797	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3730	0.11814598853192049	No Hit
ATCAGATACCGTCGTAGTTCCGACC	3614	0.11447174331216102	No Hit
GAATAACGCCGCCGCATCGCCAGTC	3528	0.11174773392509797	No Hit
GAACTACGACGGTATCTGATCGTCT	3494	0.11067079998137538	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	3482	0.11029070564829682	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3461	0.10962554056540932	No Hit
TCGTAGTTCCGACCATAAACGATGC	3254	0.1030689133198041	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2669811102618817E-4
2	0.0	0.0	0.0	0.0	1.2669811102618817E-4
3	0.0	0.0	0.0	0.0	1.2669811102618817E-4
4	0.0	0.0	0.0	3.1674527756547044E-5	1.2669811102618817E-4
5	0.0	0.0	0.0	6.334905551309409E-5	1.5837263878273522E-4
6	0.0	0.0	0.0	6.334905551309409E-5	1.5837263878273522E-4
7	0.0	0.0	0.0	6.334905551309409E-5	1.5837263878273522E-4
8	0.0	0.0	0.0	1.9004716653928227E-4	1.5837263878273522E-4
9	0.0	0.0	0.0	2.2172169429582933E-4	1.5837263878273522E-4
10	0.0	0.0	0.0	2.2172169429582933E-4	1.9004716653928227E-4
11	0.0	0.0	0.0	2.2172169429582933E-4	2.2172169429582933E-4
12	0.0	0.0	0.0	2.2172169429582933E-4	5.701414996178468E-4
13	0.0	0.0	0.0	2.2172169429582933E-4	7.918631939136761E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCT	40	0.0052892133	14.246539	4
CCGTCGT	700	0.0	12.35131	9
CGTCGTA	700	0.0	12.215581	10
ATTTGCG	445	0.0	11.957463	16
CCGACCA	600	0.0	11.87626	9
CGCATCG	645	0.0	11.784195	13
CGAACGA	275	0.0	11.747819	16
AGGCCCG	895	0.0	11.571059	10
ACCGTCG	765	0.0	11.549877	8
TCGCGTA	330	0.0	11.516373	9
CGAGGTT	350	0.0	11.397231	4
TTGCGCG	485	0.0	11.363291	18
GTCGTAG	720	0.0	11.348065	11
TTTGCGC	480	0.0	11.283521	17
ATCGCCA	685	0.0	11.235837	16
CGCAAGA	740	0.0	11.163197	2
GTCCTAA	385	0.0	11.096799	1
GAGGTTA	360	0.0	11.082575	5
GCGTAAC	370	0.0	11.041361	11
CGCGTAA	345	0.0	11.01566	10
>>END_MODULE