FastQCFastQC Report
Thu 2 Feb 2017
SRR4062334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157111
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC41980.1329696675219845No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA41310.13084747416229583No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT39960.126571412915162No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA39150.12400577616688169No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA37880.11998311114180021No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG36320.11504188481177888No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT35950.11386992728478662No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA35150.11133596506426287No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC35010.11089252167567122No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC34800.11022735659278371No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT34760.11010065848175753No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG34020.10775674342777306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG7600.020.8420814
TGTAGGA9900.017.999982
CGTCTTA5050.017.86136615
CGTCGTA10700.017.47661810
GTATCAA68450.017.1958311
TTAGGAC7550.017.1920363
AGGACGT9400.017.0850895
ACCGTCG11150.016.9685928
GACGTGA5600.016.892847
ATACCGT11250.016.817766
TACCGTC11700.016.7350257
CCGTCGT11200.016.6964119
GGACGTG9500.016.442096
GGCGTTA11550.016.19045842
TATGCCG1105.625903E-515.9999835
TACGACG12150.015.9341395
TAGCGGC13200.015.83331730
GTCGTAG11800.015.66111
ACGACGG12400.015.6128866
CGACGGT12450.015.5501837
Produced by FastQC (version 0.11.5)