##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062334_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3157111
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.314914489861142	32.0	32.0	32.0	32.0	32.0
2	31.41173116814708	32.0	32.0	32.0	32.0	32.0
3	31.501025779581397	32.0	32.0	32.0	32.0	32.0
4	31.607174723980247	32.0	32.0	32.0	32.0	32.0
5	31.52728301285574	32.0	32.0	32.0	32.0	32.0
6	35.13471049956748	36.0	36.0	36.0	36.0	36.0
7	35.15009576793467	36.0	36.0	36.0	36.0	36.0
8	35.0903056623603	36.0	36.0	36.0	36.0	36.0
9	35.22314863177126	36.0	36.0	36.0	36.0	36.0
10	35.09431787479122	36.0	36.0	36.0	36.0	36.0
11	35.23563314688651	36.0	36.0	36.0	36.0	36.0
12	35.139890868582064	36.0	36.0	36.0	36.0	36.0
13	35.19088527454372	36.0	36.0	36.0	36.0	36.0
14	35.14586658498862	36.0	36.0	36.0	36.0	36.0
15	35.10327321402384	36.0	36.0	36.0	36.0	36.0
16	35.11436341642723	36.0	36.0	36.0	36.0	36.0
17	35.08194802146646	36.0	36.0	36.0	36.0	36.0
18	35.10498522224908	36.0	36.0	36.0	36.0	36.0
19	35.08175639057353	36.0	36.0	36.0	36.0	36.0
20	35.08234585353508	36.0	36.0	36.0	36.0	36.0
21	35.07524474115734	36.0	36.0	36.0	36.0	36.0
22	35.05872394097008	36.0	36.0	36.0	36.0	36.0
23	35.012441121012216	36.0	36.0	36.0	36.0	36.0
24	34.9900421619639	36.0	36.0	36.0	36.0	36.0
25	34.95823333420966	36.0	36.0	36.0	32.0	36.0
26	34.91632540002553	36.0	36.0	36.0	32.0	36.0
27	34.8952580381241	36.0	36.0	36.0	32.0	36.0
28	34.88012933343173	36.0	36.0	36.0	32.0	36.0
29	34.85432694637598	36.0	36.0	36.0	32.0	36.0
30	34.838241037454814	36.0	36.0	36.0	32.0	36.0
31	34.84303276001382	36.0	36.0	36.0	32.0	36.0
32	34.806369177390344	36.0	36.0	36.0	32.0	36.0
33	34.7880945586012	36.0	36.0	36.0	32.0	36.0
34	34.776742724598535	36.0	36.0	36.0	32.0	36.0
35	34.74907280738625	36.0	36.0	36.0	32.0	36.0
36	34.72617560801632	36.0	36.0	36.0	32.0	36.0
37	34.71372466790049	36.0	36.0	36.0	32.0	36.0
38	34.67867902015482	36.0	36.0	36.0	32.0	36.0
39	34.66585432061147	36.0	36.0	36.0	32.0	36.0
40	34.654026735201896	36.0	36.0	36.0	32.0	36.0
41	34.621872971840396	36.0	36.0	36.0	32.0	36.0
42	34.585933785666704	36.0	36.0	36.0	32.0	36.0
43	34.5898192999866	36.0	36.0	36.0	32.0	36.0
44	34.559836825502806	36.0	36.0	36.0	32.0	36.0
45	34.512012406279034	36.0	36.0	36.0	32.0	36.0
46	34.52830483312117	36.0	36.0	36.0	32.0	36.0
47	34.49817443859275	36.0	36.0	36.0	32.0	36.0
48	34.46725028039876	36.0	36.0	36.0	32.0	36.0
49	34.46108039913706	36.0	36.0	36.0	32.0	36.0
50	33.952604770627325	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	17.0
21	89.0
22	238.0
23	699.0
24	1903.0
25	4583.0
26	9482.0
27	17777.0
28	29921.0
29	46341.0
30	68190.0
31	98653.0
32	148484.0
33	246792.0
34	560083.0
35	1923857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60072013895907	17.7739874986259	12.137439717824137	26.487852644590898
2	16.546187410416618	19.864775615898132	36.28406940187997	27.30496757180529
3	18.57453586228588	23.49352616063332	27.97350472317713	29.95843325390367
4	12.65360977629203	15.806242867283244	35.12953472910485	36.410612627319885
5	15.342476080188503	35.87048412298459	32.469811799458434	16.31722799736848
6	35.30792034079388	34.377065780959036	16.489885176232992	13.825128702014094
7	31.41244093884627	29.625521323463964	20.218750762168806	18.74328697552096
8	28.88137287539146	31.825330183195966	19.060273775613208	20.233023165799366
9	27.60507945396915	14.507472179470406	18.3826606033174	39.504787763243044
10	16.801974970154674	25.79320144271139	30.01699971904694	27.387823868086997
11	37.8836537581352	20.69556629462822	21.830401275089788	19.590378672146784
12	25.631091209653384	23.121360002863376	27.54059011545682	23.706958672026417
13	29.607131329877216	18.83177373237748	25.02987699830636	26.53121793943894
14	24.278652223504338	19.618695699961137	23.981228407870358	32.121423668664164
15	25.937193845892654	26.70346402137904	21.52274658699045	25.83659554573786
16	26.87203585809938	25.127371194741016	23.01094259910405	24.989650348055548
17	24.76545804059471	25.377504940434463	24.56866419964328	25.28837281932754
18	25.855917007669355	23.919431404217338	25.63862974725944	24.58602184085387
19	26.223373204173058	24.556532855512526	24.54345127554907	24.67664266476535
20	26.328405938213766	23.60043723518115	24.294014369466264	25.77714245713882
21	27.574861954489403	23.568857731007874	23.55026478321478	25.30601553128794
22	26.370406362018944	23.795679024272506	24.14587260314889	25.688042010559652
23	24.727987074258714	23.542947967303018	24.857662590894016	26.87140236754425
24	25.301549422874263	24.505536865824485	24.38878455651385	25.804129154787397
25	25.554343828899267	23.83745772638339	24.54006210107912	26.06813634363822
26	25.108176430920548	24.749082309744573	25.062501761895607	25.080239497439273
27	26.129553252958164	24.086641236244148	24.18837981939818	25.59542569139951
28	25.093606148152535	23.969793903350247	25.01853751736952	25.918062431127698
29	25.01698546550945	24.206719370969218	24.96320211737883	25.813093046142505
30	25.04248346035347	24.324136845362737	25.371328407521943	25.262051286761857
31	25.84340556920552	24.109478570756618	23.932544658708547	26.11457120132932
32	25.37617461026869	24.197597106975334	23.97967635601029	26.446551926745688
33	24.91220612769079	23.852566476123265	24.715127215989554	26.520100180196387
34	25.75300646698833	24.02956373722685	24.952907895857955	25.264521899926862
35	26.501412208820025	23.855480532676868	24.723837711122606	24.9192695473805
36	25.080777964411133	24.503382997937038	24.400060688395182	26.015778349256646
37	26.38523004100901	24.194746399477243	24.167886399939693	25.252137159574055
38	25.263603338621927	24.001278383940257	24.716679267849624	26.018439009588196
39	25.886419577898906	23.908598715724597	24.115591754613632	26.089389951762858
40	26.251088415960034	24.14732963142569	24.6079406140614	24.993641338552873
41	24.926871434042074	24.485771960504398	25.23652161739008	25.350834988063454
42	26.621300296378557	24.699733395499873	24.327399321721664	24.35156698639991
43	25.503062768461422	23.551721811491582	24.69251160317138	26.252703816875616
44	25.136461784207143	24.258475549323418	24.48646880011504	26.118593866354402
45	25.26686581498085	24.491663422667116	24.58073219471853	25.660738567633512
46	25.148149872652397	24.005869930920934	24.89434156016885	25.95163863625781
47	25.48824716275328	23.86951990903073	25.18413992543814	25.458093002777858
48	26.22616056261563	24.80014164848813	23.7997650383531	25.173932750543138
49	25.07548831827579	24.64911750014491	24.4652151919904	25.8101789895889
50	25.02864169172386	25.070578766473528	24.156927013335928	25.743852528466686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	30.5
2	30.0
3	103.0
4	176.0
5	216.0
6	256.0
7	255.5
8	255.0
9	346.5
10	438.0
11	678.5
12	919.0
13	1637.0
14	2355.0
15	3297.5
16	4240.0
17	4932.0
18	5624.0
19	5929.5
20	6235.0
21	7322.0
22	8409.0
23	9010.0
24	9611.0
25	11755.0
26	13899.0
27	18595.0
28	23291.0
29	27474.0
30	31657.0
31	35594.0
32	39531.0
33	46439.0
34	53347.0
35	63181.5
36	73016.0
37	90856.5
38	108697.0
39	118741.0
40	128785.0
41	141847.0
42	154909.0
43	157310.5
44	159712.0
45	179443.0
46	199174.0
47	216649.5
48	234125.0
49	241105.0
50	248085.0
51	236881.5
52	225678.0
53	224135.5
54	222593.0
55	229117.0
56	235641.0
57	232193.5
58	228746.0
59	210416.0
60	192086.0
61	170218.5
62	148351.0
63	131346.5
64	114342.0
65	97512.0
66	80682.0
67	71586.5
68	62491.0
69	57937.5
70	53384.0
71	41057.5
72	28731.0
73	25854.0
74	22977.0
75	16646.0
76	10315.0
77	9491.5
78	8668.0
79	7443.5
80	6219.0
81	4907.5
82	3596.0
83	3167.5
84	2739.0
85	2275.0
86	1811.0
87	1353.0
88	895.0
89	563.5
90	232.0
91	148.0
92	64.0
93	46.0
94	28.0
95	21.5
96	15.0
97	12.5
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01577391482276043
2	0.004307735774890398
3	6.334905551309409E-4
4	1.2669811102618817E-4
5	0.0
6	3.8009433307856455E-4
7	6.334905551309409E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.2669811102618817E-4
47	3.1674527756547044E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3157111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.7864653512539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.84724947164868	35.18563925272807
2	13.245520441887853	12.129311255436471
3	4.094404053691218	5.6240486801509935
4	1.780635982207299	3.261161108101218
5	0.9606078236324536	2.199141841644537
6	0.6110362854465524	1.6786315027174474
7	0.39621032186269967	1.2698749121642958
8	0.281060818322651	1.0295025135780103
9	0.23562005121747998	0.9709388380016835
>10	1.324960294650485	11.210344723869545
>50	0.10468263529689699	3.3201847130326874
>100	0.09098450254473547	8.740854744466509
>500	0.016952867589234304	5.408447176865186
>1k	0.010074450001682722	7.9719187372432945
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	4198	0.1329696675219845	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	4131	0.13084747416229583	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3996	0.126571412915162	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3915	0.12400577616688169	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	3788	0.11998311114180021	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	3632	0.11504188481177888	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3595	0.11386992728478662	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	3515	0.11133596506426287	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3501	0.11089252167567122	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3480	0.11022735659278371	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	3476	0.11010065848175753	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	3402	0.10775674342777306	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1674527756547044E-5
2	0.0	0.0	0.0	0.0	3.1674527756547044E-5
3	0.0	0.0	0.0	0.0	3.1674527756547044E-5
4	0.0	0.0	0.0	3.1674527756547044E-5	3.1674527756547044E-5
5	0.0	0.0	0.0	6.334905551309409E-5	9.502358326964114E-5
6	0.0	0.0	0.0	6.334905551309409E-5	1.2669811102618817E-4
7	0.0	0.0	0.0	6.334905551309409E-5	1.2669811102618817E-4
8	0.0	0.0	0.0	1.2669811102618817E-4	1.2669811102618817E-4
9	0.0	0.0	0.0	1.5837263878273522E-4	1.2669811102618817E-4
10	0.0	0.0	0.0	1.9004716653928227E-4	1.2669811102618817E-4
11	0.0	0.0	0.0	1.9004716653928227E-4	1.2669811102618817E-4
12	0.0	0.0	0.0	1.9004716653928227E-4	3.8009433307856455E-4
13	0.0	0.0	0.0	1.9004716653928227E-4	4.4344338859165865E-4
14	0.0	0.0	0.0	1.9004716653928227E-4	5.384669718612998E-4
15	6.334905551309409E-5	0.0	0.0	2.2172169429582933E-4	6.65165082887488E-4
16	6.334905551309409E-5	0.0	0.0	2.2172169429582933E-4	7.601886661571291E-4
17	6.334905551309409E-5	0.0	0.0	2.5339622205237635E-4	7.601886661571291E-4
18	6.334905551309409E-5	0.0	0.0	3.484198053220175E-4	7.918631939136761E-4
19	6.334905551309409E-5	0.0	0.0	4.751179163482057E-4	7.918631939136761E-4
20	6.334905551309409E-5	0.0	0.0	6.018160273743939E-4	0.0010452594159660526
21	6.334905551309409E-5	0.0	0.0	7.918631939136761E-4	0.0011719575269922406
22	6.334905551309409E-5	0.0	0.0	0.0010769339437225996	0.0012669811102618818
23	6.334905551309409E-5	0.0	0.0	0.0015203773323142582	0.001362004693531523
24	6.334905551309409E-5	0.0	0.0	0.0024706131650106696	0.001362004693531523
25	6.334905551309409E-5	0.0	0.0	0.0029457310813588754	0.001362004693531523
26	6.334905551309409E-5	0.0	0.0	0.003579221636489816	0.0014570282768011642
27	9.502358326964114E-5	0.0	0.0	0.004624481052455868	0.0014887028045577112
28	9.502358326964114E-5	0.0	0.0	0.007095094217466538	0.0015203773323142582
29	9.502358326964114E-5	0.0	0.0	0.011751249797678954	0.0015203773323142582
30	9.502358326964114E-5	0.0	0.0	0.020620117569512125	0.0015520518600708052
31	9.502358326964114E-5	0.0	0.0	0.04770183880135985	0.0015837263878273522
32	9.502358326964114E-5	0.0	0.0	0.07636728642103492	0.0016154009155838994
33	9.502358326964114E-5	0.0	0.0	0.1066481349562939	0.0016154009155838994
34	1.2669811102618817E-4	0.0	0.0	0.1425353749044617	0.0016154009155838994
35	1.2669811102618817E-4	0.0	0.0	0.1845357987096431	0.0016154009155838994
36	1.2669811102618817E-4	0.0	0.0	0.24281692978168964	0.0016154009155838994
37	1.2669811102618817E-4	0.0	0.0	0.3314422584445083	0.0016154009155838994
38	1.2669811102618817E-4	0.0	0.0	0.4548778931117721	0.0016470754433404464
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	760	0.0	20.842081	4
TGTAGGA	990	0.0	17.99998	2
CGTCTTA	505	0.0	17.861366	15
CGTCGTA	1070	0.0	17.476618	10
GTATCAA	6845	0.0	17.195831	1
TTAGGAC	755	0.0	17.192036	3
AGGACGT	940	0.0	17.085089	5
ACCGTCG	1115	0.0	16.968592	8
GACGTGA	560	0.0	16.89284	7
ATACCGT	1125	0.0	16.81776	6
TACCGTC	1170	0.0	16.735025	7
CCGTCGT	1120	0.0	16.696411	9
GGACGTG	950	0.0	16.44209	6
GGCGTTA	1155	0.0	16.190458	42
TATGCCG	110	5.625903E-5	15.999983	5
TACGACG	1215	0.0	15.934139	5
TAGCGGC	1320	0.0	15.833317	30
GTCGTAG	1180	0.0	15.661	11
ACGACGG	1240	0.0	15.612886	6
CGACGGT	1245	0.0	15.550183	7
>>END_MODULE