FastQCFastQC Report
Thu 2 Feb 2017
SRR4062333_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062333_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338695
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG180025.315106511758367No Hit
TCCATGTACTCTGCGTTGATACCAC153854.5424349340852395No Hit
GAGTACATGGAAGCAGTGGTATCAA73302.1641890196194216No Hit
CATGTACTCTGCGTTGATACCACTG68012.0080012991039133No Hit
GTATCAACGCAGAGTACTTTTTTTT60421.7839058740164455No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55011.6241751428276177No Hit
CCCATGTACTCTGCGTTGATACCAC49271.454701132287161No Hit
GTACATGGGAAGCAGTGGTATCAAC46921.3853171732679845No Hit
GCTTCCATGTACTCTGCGTTGATAC45721.349887066534788No Hit
GCGTTGATACCACTGCTTCCATGTA43051.2710550790534256No Hit
TATCAACGCAGAGTACTTTTTTTTT42241.2471397570085179No Hit
CATGGAAGCAGTGGTATCAACGCAG39181.1567929848388667No Hit
GAGTACTTTTTTTTTTTTTTTTTTT36941.0906567856035667No Hit
GTATCAACGCAGAGTACATGGAAGC33860.9997195116550289No Hit
ACTCTGCGTTGATACCACTGCTTCC28140.8308360028934586No Hit
CAGTGGTATCAACGCAGAGTACATG27090.7998346595019117No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26860.7930438890447157No Hit
ACGCAGAGTACATGGAAGCAGTGGT26520.7830053588036434No Hit
GTACATGGGGTGGTATCAACGCAAA23690.6994493570911882No Hit
GTATCAACGCAGAGTACATGGGAAG23680.699154106201745No Hit
TATCAACGCAGAGTACATGGAAGCA22030.6504377094435997No Hit
GGTATCAACGCAGAGTACTTTTTTT19570.5778059906405468No Hit
ATACCACTGCTTCCATGTACTCTGC17330.5116697914052466No Hit
GCGTTGATACCACTGCTTCCCATGT17150.5063552753952671No Hit
GAGTACATGGGAAGCAGTGGTATCA15960.4712204195515139No Hit
GGTATCAACGCAGAGTACATGGAAG14870.43903807260219374No Hit
TATCAACGCAGAGTACATGGGAAGC14580.43047579680833786No Hit
GTACATGGGTGGTATCAACGCAAAA14440.42634228435613164No Hit
GTACTCTGCGTTGATACCACTGCTT13680.40390321675844054No Hit
AAGCAGTGGTATCAACGCAGAGTAC12930.38175940005019265No Hit
AAAAAGTACTCTGCGTTGATACCAC12760.37674013492965647No Hit
CCACTGCTTCCATGTACTCTGCGTT12410.3664063537991408No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11970.3534153146636354No Hit
GTGGTATCAACGCAGAGTACATGGA11500.33953852285980013No Hit
GAAGCAGTGGTATCAACGCAGAGTA11350.3351097595181506No Hit
GTACATGGGAGTGGTATCAACGCAA11340.33481450862870726No Hit
GCTTCCCATGTACTCTGCGTTGATA11200.330680996176501No Hit
GGTATCAACGCAGAGTACATGGGAA11150.32920474172928443No Hit
ACGCAGAGTACATGGGAAGCAGTGG10950.32329972394041834No Hit
GTATCAACGCAGAGTACATGGGGTG10870.32093771682487193No Hit
ACATGGAAGCAGTGGTATCAACGCA10420.30765142679992324No Hit
CTGCTTCCATGTACTCTGCGTTGAT9430.2784215887450361No Hit
GTTGATACCACTGCTTCCATGTACT9060.2674973058356338No Hit
TGGAAGCAGTGGTATCAACGCAGAG8540.252144259584582No Hit
GATACCACTGCTTCCATGTACTCTG8080.23856271867019No Hit
GCAGAGTACATGGAAGCAGTGGTAT7620.22498117775579798No Hit
TATCAACGCAGAGTACATGGGGTGG7360.21730465463027207No Hit
CCCCATGTACTCTGCGTTGATACCA7030.20756137527864305No Hit
GCAGTGGTATCAACGCAGAGTACAT6910.20401836460532335No Hit
ATCAACGCAGAGTACTTTTTTTTTT6770.19988485215311713No Hit
CCATGTACTCTGCGTTGATACCACT6270.1851223076809519No Hit
CTTCCATGTACTCTGCGTTGATACC6210.18335080234429205No Hit
GTATCAACGCAGAGTACATGGGAGT6110.18039829344985903No Hit
ATCAACGCAGAGTACATGGAAGCAG5910.17449327566099293No Hit
GTGGTATCAACGCAGAGTACATGGG5750.16976926142990006No Hit
AAAGTACTCTGCGTTGATACCACTG5480.16179748741493083No Hit
GTATCAACGCAGAGTACATGGGTGG5160.1523494589527451No Hit
CTGCTTCCCATGTACTCTGCGTTGA5050.14910169916886876No Hit
GGTATCAACGCAGAGTACATGGGGT4930.1455586884955491No Hit
TGATACCACTGCTTCCATGTACTCT4780.14112992515389952No Hit
GATACCACTGCTTCCCATGTACTCT4750.1402441724855696No Hit
CACTGCTTCCATGTACTCTGCGTTG4620.13640591092280666No Hit
GTATCAACGCAGAGTACATGGGCAG4580.13522490736503343No Hit
ACGCAGAGTACATGGGGTGGTATCA4540.13404390380726022No Hit
ATACCACTGCTTCCCATGTACTCTG4230.12489112623451779No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT4150.12252911911897135No Hit
GAGTACATGGGGTGGTATCAACGCA4010.11839560666676509No Hit
TCCATGTACTCTGCGTTGATACCAA3970.11721460310899186No Hit
TATCAACGCAGAGTACATGGGAGTG3870.11426209421455881No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3820.11278583976734231No Hit
GGGAAGCAGTGGTATCAACGCAGAG3690.10894757820457934No Hit
TACCACTGCTTCCCATGTACTCTGC3500.10333781130515657No Hit
CTGCGTTGATACCACTGCTTCCATG3400.10038530241072352No Hit
TATCAACGCAGAGTACATGGGTGGT3390.1000900515212802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCA250.006019563419.00162714
GCGTCCC250.006019563419.00162713
TATCGGT250.006019563419.00162715
TTGTGCG250.00602470718.9988167
TGTGCGC250.00602470718.9988168
TGCGCTA250.00602470718.99881610
CAGGCTA250.00602470718.9988169
GCGCTAT250.00602470718.99881611
AAAAAGT2200.015.9715561
GTACATA551.1269283E-515.5398921
ATACCAA1101.0913936E-1114.685245519
AAAAGTA2450.014.34604552
CGGTCTC400.00525965614.25332718
TCGGTCT400.00525965614.25332717
ATACCAT400.005269987514.2491136
TAGAGTG500.001496023713.2991715
AATCCCA500.001496023713.2991716
AGCCCTG853.920348E-612.2933525
CAGCACT701.0866741E-412.21352611
AATACAT550.003061348812.0901572
Produced by FastQC (version 0.11.5)