##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062333_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338695
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.02896411225439	32.0	32.0	32.0	21.0	32.0
2	30.28958502487489	32.0	32.0	32.0	21.0	32.0
3	30.394709104061175	32.0	32.0	32.0	27.0	32.0
4	30.125003321572507	32.0	32.0	32.0	21.0	32.0
5	30.35511595978683	32.0	32.0	32.0	27.0	32.0
6	33.662218219932384	36.0	36.0	36.0	27.0	36.0
7	33.25687713134236	36.0	36.0	36.0	21.0	36.0
8	33.17794771106748	36.0	36.0	36.0	14.0	36.0
9	33.07521811659458	36.0	36.0	36.0	14.0	36.0
10	33.21973456945039	36.0	36.0	36.0	21.0	36.0
11	33.57242651943489	36.0	36.0	36.0	27.0	36.0
12	33.435533444544504	36.0	36.0	36.0	21.0	36.0
13	33.44058813977177	36.0	36.0	36.0	21.0	36.0
14	33.391579444633074	36.0	36.0	36.0	21.0	36.0
15	33.52990743884616	36.0	36.0	36.0	27.0	36.0
16	33.38727763917389	36.0	36.0	36.0	21.0	36.0
17	33.3017759341	36.0	36.0	36.0	21.0	36.0
18	33.406333131578556	36.0	36.0	36.0	21.0	36.0
19	33.373799436070804	36.0	36.0	36.0	21.0	36.0
20	33.14366317778532	36.0	36.0	36.0	14.0	36.0
21	32.939122219105684	36.0	36.0	36.0	14.0	36.0
22	32.95276281019797	36.0	36.0	36.0	14.0	36.0
23	32.870036463484844	36.0	36.0	36.0	14.0	36.0
24	32.99762323033998	36.0	36.0	36.0	14.0	36.0
25	32.26373285699523	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	17.0
5	28.0
6	71.0
7	20.0
8	49.0
9	62.0
10	44.0
11	19.0
12	27.0
13	20.0
14	338.0
15	351.0
16	433.0
17	650.0
18	939.0
19	1315.0
20	1916.0
21	2622.0
22	3392.0
23	4334.0
24	4923.0
25	5468.0
26	6482.0
27	7378.0
28	8808.0
29	10634.0
30	13276.0
31	17565.0
32	23562.0
33	31731.0
34	64434.0
35	127781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.74262834330394	15.91616419911298	14.559944723647767	18.78126273393531
2	9.515331355093966	25.178262987731966	38.737469920427536	26.568935736746536
3	20.77413220895487	34.91744322006874	25.66553283964615	18.642891731330238
4	11.618601574542726	19.25655124351077	31.8021013589733	37.322745822973204
5	10.141627161697162	42.13725577067919	32.06917430921684	15.651942758406806
6	29.50099552751142	30.638910059849817	22.788717748748944	17.071376663889826
7	24.36092891331324	34.616793712698694	23.52803876381256	17.4942386101755
8	32.2334102737641	27.508223562686744	24.0060054912415	16.25236067230765
9	29.09863684191859	18.012189210597874	18.580270267074944	34.30890368040859
10	16.460759358952757	30.370810532757442	31.91578051138329	21.25264959690651
11	31.601337428790195	25.685787010621976	17.89771803429896	24.81515752628887
12	22.0908744156616	28.17420306738852	29.86697338580675	19.86794913114313
13	34.09328753111457	23.181460743911597	24.5578923096025	18.167359415371333
14	27.801165994583922	19.11296902900796	26.919574754916454	26.166290221491668
15	27.41808657814144	22.38208132811484	34.830793061451324	15.369039032292392
16	23.651616641146457	26.617971350363884	30.72357180440682	19.006840204082838
17	21.385451341875136	25.23310609061722	33.06204140035121	20.319401167156432
18	24.899406044802376	21.324144618777733	36.82581354714538	16.95063578927451
19	19.176891464388138	35.84986547615528	29.54587115276587	15.427371906690713
20	20.96489049713826	20.414715481765292	41.088406414076914	17.531987607019538
21	22.110202138762332	29.17101524688602	25.244136438490628	23.474646175861018
22	18.965466255818217	30.439256924196094	27.86179405137183	22.73348276861386
23	17.582963473303547	29.584465583058094	27.202675411232114	25.629895532406245
24	16.32605391852496	34.8876746675182	28.3376796939216	20.448591720035246
25	24.5679702213589	25.972344181013458	25.17111187982107	24.288573717806575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	565.0
1	565.0
2	1020.5
3	1476.0
4	1476.0
5	1476.0
6	4095.5
7	6715.0
8	6715.0
9	6715.0
10	6053.5
11	5392.0
12	5392.0
13	5392.0
14	3566.0
15	1740.0
16	1740.0
17	1740.0
18	2748.0
19	3756.0
20	3756.0
21	3756.0
22	5298.0
23	6840.0
24	6840.0
25	6840.0
26	9377.5
27	11915.0
28	11915.0
29	11915.0
30	13773.5
31	15632.0
32	15632.0
33	15632.0
34	15024.0
35	14416.0
36	14416.0
37	14416.0
38	16079.5
39	17743.0
40	17743.0
41	17743.0
42	28591.5
43	39440.0
44	39440.0
45	39440.0
46	78719.0
47	117998.0
48	117998.0
49	117998.0
50	86116.0
51	54234.0
52	54234.0
53	54234.0
54	35910.0
55	17586.0
56	17586.0
57	17586.0
58	13652.5
59	9719.0
60	9719.0
61	9719.0
62	7942.0
63	6165.0
64	6165.0
65	6165.0
66	4919.5
67	3674.0
68	3674.0
69	3674.0
70	2791.5
71	1909.0
72	1909.0
73	1909.0
74	1444.0
75	979.0
76	979.0
77	979.0
78	692.5
79	406.0
80	406.0
81	406.0
82	272.0
83	138.0
84	138.0
85	138.0
86	92.5
87	47.0
88	47.0
89	47.0
90	33.5
91	20.0
92	20.0
93	20.0
94	17.0
95	14.0
96	14.0
97	14.0
98	95.0
99	176.0
100	176.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009743279351629046
2	0.0029525088944330445
3	0.005609766899422785
4	0.01682930069826835
5	0.038382615627629575
6	0.0534404109892381
7	0.06938395901917654
8	0.0997948006318369
9	0.10717607286791951
10	0.12872938779728074
11	0.12813888601839413
12	0.1452634376061058
13	0.1266626315711776
14	0.13079614402338388
15	0.12134811556119812
16	0.11839560666676509
17	0.12902463868672404
18	0.13404390380726022
19	0.13729166359113656
20	0.12961514046561065
21	0.13640591092280666
22	0.15736872407328126
23	0.14880644827942544
24	0.14201567782222946
25	0.13758691448057986
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	338695.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.58684923667868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67699999166626	23.573913863842787
2	11.801999998765583	6.983679893095185
3	3.432999999640929	3.047149602566824
4	1.48200001025252	1.7539084348839027
5	0.8029999999675925	1.1879119968047074
6	0.48099999994997295	0.8538764688817381
7	0.35699999996266185	0.73937536234727
8	0.26599999997217805	0.6296081516906691
9	0.2139999999776169	0.5698427162388293
>10	1.198999999874592	6.925985537089696
>50	0.11099999998839007	2.27848325947831
>100	0.1169999999877625	7.4023338097504
>500	0.016999999998221903	3.4539687795286054
>1k	0.03499999999633921	23.125673097953985
>5k	0.003999999999581625	7.596127672230375
>10k+	0.0019999999997908123	9.878161353616715
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	18002	5.315106511758367	No Hit
TCCATGTACTCTGCGTTGATACCAC	15385	4.5424349340852395	No Hit
GAGTACATGGAAGCAGTGGTATCAA	7330	2.1641890196194216	No Hit
CATGTACTCTGCGTTGATACCACTG	6801	2.0080012991039133	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6042	1.7839058740164455	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5501	1.6241751428276177	No Hit
CCCATGTACTCTGCGTTGATACCAC	4927	1.454701132287161	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4692	1.3853171732679845	No Hit
GCTTCCATGTACTCTGCGTTGATAC	4572	1.349887066534788	No Hit
GCGTTGATACCACTGCTTCCATGTA	4305	1.2710550790534256	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4224	1.2471397570085179	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3918	1.1567929848388667	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3694	1.0906567856035667	No Hit
GTATCAACGCAGAGTACATGGAAGC	3386	0.9997195116550289	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2814	0.8308360028934586	No Hit
CAGTGGTATCAACGCAGAGTACATG	2709	0.7998346595019117	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2686	0.7930438890447157	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2652	0.7830053588036434	No Hit
GTACATGGGGTGGTATCAACGCAAA	2369	0.6994493570911882	No Hit
GTATCAACGCAGAGTACATGGGAAG	2368	0.699154106201745	No Hit
TATCAACGCAGAGTACATGGAAGCA	2203	0.6504377094435997	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1957	0.5778059906405468	No Hit
ATACCACTGCTTCCATGTACTCTGC	1733	0.5116697914052466	No Hit
GCGTTGATACCACTGCTTCCCATGT	1715	0.5063552753952671	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1596	0.4712204195515139	No Hit
GGTATCAACGCAGAGTACATGGAAG	1487	0.43903807260219374	No Hit
TATCAACGCAGAGTACATGGGAAGC	1458	0.43047579680833786	No Hit
GTACATGGGTGGTATCAACGCAAAA	1444	0.42634228435613164	No Hit
GTACTCTGCGTTGATACCACTGCTT	1368	0.40390321675844054	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1293	0.38175940005019265	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1276	0.37674013492965647	No Hit
CCACTGCTTCCATGTACTCTGCGTT	1241	0.3664063537991408	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1197	0.3534153146636354	No Hit
GTGGTATCAACGCAGAGTACATGGA	1150	0.33953852285980013	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1135	0.3351097595181506	No Hit
GTACATGGGAGTGGTATCAACGCAA	1134	0.33481450862870726	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1120	0.330680996176501	No Hit
GGTATCAACGCAGAGTACATGGGAA	1115	0.32920474172928443	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1095	0.32329972394041834	No Hit
GTATCAACGCAGAGTACATGGGGTG	1087	0.32093771682487193	No Hit
ACATGGAAGCAGTGGTATCAACGCA	1042	0.30765142679992324	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	943	0.2784215887450361	No Hit
GTTGATACCACTGCTTCCATGTACT	906	0.2674973058356338	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	854	0.252144259584582	No Hit
GATACCACTGCTTCCATGTACTCTG	808	0.23856271867019	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	762	0.22498117775579798	No Hit
TATCAACGCAGAGTACATGGGGTGG	736	0.21730465463027207	No Hit
CCCCATGTACTCTGCGTTGATACCA	703	0.20756137527864305	No Hit
GCAGTGGTATCAACGCAGAGTACAT	691	0.20401836460532335	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	677	0.19988485215311713	No Hit
CCATGTACTCTGCGTTGATACCACT	627	0.1851223076809519	No Hit
CTTCCATGTACTCTGCGTTGATACC	621	0.18335080234429205	No Hit
GTATCAACGCAGAGTACATGGGAGT	611	0.18039829344985903	No Hit
ATCAACGCAGAGTACATGGAAGCAG	591	0.17449327566099293	No Hit
GTGGTATCAACGCAGAGTACATGGG	575	0.16976926142990006	No Hit
AAAGTACTCTGCGTTGATACCACTG	548	0.16179748741493083	No Hit
GTATCAACGCAGAGTACATGGGTGG	516	0.1523494589527451	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	505	0.14910169916886876	No Hit
GGTATCAACGCAGAGTACATGGGGT	493	0.1455586884955491	No Hit
TGATACCACTGCTTCCATGTACTCT	478	0.14112992515389952	No Hit
GATACCACTGCTTCCCATGTACTCT	475	0.1402441724855696	No Hit
CACTGCTTCCATGTACTCTGCGTTG	462	0.13640591092280666	No Hit
GTATCAACGCAGAGTACATGGGCAG	458	0.13522490736503343	No Hit
ACGCAGAGTACATGGGGTGGTATCA	454	0.13404390380726022	No Hit
ATACCACTGCTTCCCATGTACTCTG	423	0.12489112623451779	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	415	0.12252911911897135	No Hit
GAGTACATGGGGTGGTATCAACGCA	401	0.11839560666676509	No Hit
TCCATGTACTCTGCGTTGATACCAA	397	0.11721460310899186	No Hit
TATCAACGCAGAGTACATGGGAGTG	387	0.11426209421455881	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	382	0.11278583976734231	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	369	0.10894757820457934	No Hit
TACCACTGCTTCCCATGTACTCTGC	350	0.10333781130515657	No Hit
CTGCGTTGATACCACTGCTTCCATG	340	0.10038530241072352	No Hit
TATCAACGCAGAGTACATGGGTGGT	339	0.1000900515212802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.9525088944330443E-4	0.0
12	0.0	0.0	0.0	2.9525088944330443E-4	0.0
13	0.0	0.0	0.0	2.9525088944330443E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCCA	25	0.0060195634	19.001627	14
GCGTCCC	25	0.0060195634	19.001627	13
TATCGGT	25	0.0060195634	19.001627	15
TTGTGCG	25	0.006024707	18.998816	7
TGTGCGC	25	0.006024707	18.998816	8
TGCGCTA	25	0.006024707	18.998816	10
CAGGCTA	25	0.006024707	18.998816	9
GCGCTAT	25	0.006024707	18.998816	11
AAAAAGT	220	0.0	15.971556	1
GTACATA	55	1.1269283E-5	15.539892	1
ATACCAA	110	1.0913936E-11	14.6852455	19
AAAAGTA	245	0.0	14.3460455	2
CGGTCTC	40	0.005259656	14.253327	18
TCGGTCT	40	0.005259656	14.253327	17
ATACCAT	40	0.0052699875	14.249113	6
TAGAGTG	50	0.0014960237	13.299171	5
AATCCCA	50	0.0014960237	13.299171	6
AGCCCTG	85	3.920348E-6	12.293352	5
CAGCACT	70	1.0866741E-4	12.213526	11
AATACAT	55	0.0030613488	12.090157	2
>>END_MODULE