FastQCFastQC Report
Thu 2 Feb 2017
SRR4062332_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062332_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349722
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG38221.0928680494793006No Hit
TCCATGTACTCTGCGTTGATACCAC32390.9261642104299987No Hit
GAGTACATGGAAGCAGTGGTATCAA16570.47380490789827345No Hit
CATGTACTCTGCGTTGATACCACTG14590.41718850973058597No Hit
GTACATGGGAAGCAGTGGTATCAAC13860.39631478717381236No Hit
CCCATGTACTCTGCGTTGATACCAC11860.33912650619635026No Hit
GTATCAACGCAGAGTACTTTTTTTT11700.33455144371815326No Hit
GCTTCCATGTACTCTGCGTTGATAC11120.3179668422346893No Hit
CTGTAGGACGTGGAATATGGCAAGA9700.27736316274069117No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9170.2622082682816637No Hit
TATCAACGCAGAGTACTTTTTTTTT8580.24533772539331242No Hit
GTCCTAAAGTGTGTATTTCTCATTT8340.23847513167601694No Hit
GCGTTGATACCACTGCTTCCATGTA8310.237617307461355No Hit
GTCCTACAGTGGACATTTCTAAATT8260.2361876004369185No Hit
CATGGAAGCAGTGGTATCAACGCAG8160.23332818638804537No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8080.23104065514894687No Hit
GTATCAACGCAGAGTACATGGGAAG6500.18586191317675183No Hit
ACTCTGCGTTGATACCACTGCTTCC6050.17299454995682284No Hit
CTTTAGGACGTGAAATATGGCGAGG5960.17042107731283707No Hit
CAGTGGTATCAACGCAGAGTACATG5890.16841948747862587No Hit
GGTATCAACGCAGAGTACTTTTTTT5210.14897547194628877No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5020.14354258525342986No Hit
GTATCAACGCAGAGTACATGGAAGC4980.14239881963388062No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4660.1332486946774867No Hit
GAGTACATGGGAAGCAGTGGTATCA4590.1312471048432755No Hit
GTCCTACAGTGTGCATTTCTCATTT4310.12324074550643083No Hit
ACGCAGAGTACATGGAAGCAGTGGT4160.11895162443312116No Hit
GGTATCAACGCAGAGTACATGGGAA3760.10751396823762875No Hit
TATCAACGCAGAGTACATGGGAAGC3680.10522643699853026No Hit
GCGTTGATACCACTGCTTCCCATGT3670.10494049559364295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTCA456.732964E-414.7808994
GAAATGT1300.014.6184716
ATAGGGT400.005260877314.2530093
TAGGGTC400.005260877314.2530094
TAGGACC1350.014.0770474
CATAGGG551.9569349E-413.8151612
TGTCCAC1450.013.76152710
AATGTCC1400.013.5742958
TTAGAAA1351.8189894E-1213.3731953
CTACACT500.001493108613.3028094
GGACCGC500.001493108613.3028096
TCCAACG500.001497954813.297091518
CTCTAGG500.001502814613.2913811
ATAGGAC655.4181426E-513.1566243
GTGTAGA655.4620352E-513.1453221
AAATGTC1501.8189894E-1212.6693427
TTTAGAA1600.012.4660242
ATGTCCA1551.8189894E-1212.26065359
GACAGTG550.003055418412.0934637
ACACCAT550.003055418412.0934636
Produced by FastQC (version 0.11.5)