##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062332_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	349722
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.171570561760483	32.0	32.0	32.0	32.0	32.0
2	30.89135084438497	32.0	32.0	32.0	32.0	32.0
3	30.89926570247225	32.0	32.0	32.0	32.0	32.0
4	30.882092061694717	32.0	32.0	32.0	32.0	32.0
5	30.89942011083089	32.0	32.0	32.0	32.0	32.0
6	34.49003494203968	36.0	36.0	36.0	32.0	36.0
7	34.43002155998193	36.0	36.0	36.0	32.0	36.0
8	34.3794699790119	36.0	36.0	36.0	32.0	36.0
9	34.425683828869786	36.0	36.0	36.0	32.0	36.0
10	34.268833530632904	36.0	36.0	36.0	32.0	36.0
11	34.436240785538224	36.0	36.0	36.0	32.0	36.0
12	34.32953603147643	36.0	36.0	36.0	32.0	36.0
13	34.30349820714739	36.0	36.0	36.0	32.0	36.0
14	34.26470739616038	36.0	36.0	36.0	32.0	36.0
15	34.21155088899183	36.0	36.0	36.0	32.0	36.0
16	34.186239355831205	36.0	36.0	36.0	32.0	36.0
17	34.14503805880099	36.0	36.0	36.0	32.0	36.0
18	34.10198386146711	36.0	36.0	36.0	32.0	36.0
19	34.11080515380788	36.0	36.0	36.0	32.0	36.0
20	34.08785549665163	36.0	36.0	36.0	32.0	36.0
21	34.01023098346687	36.0	36.0	36.0	32.0	36.0
22	33.95093531433539	36.0	36.0	36.0	32.0	36.0
23	33.876135902230914	36.0	36.0	36.0	32.0	36.0
24	33.839604028342514	36.0	36.0	36.0	32.0	36.0
25	33.36677704004895	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	4.0
5	33.0
6	69.0
7	14.0
8	58.0
9	49.0
10	23.0
11	9.0
12	12.0
13	11.0
14	112.0
15	128.0
16	177.0
17	231.0
18	327.0
19	381.0
20	570.0
21	775.0
22	1158.0
23	1562.0
24	2084.0
25	2783.0
26	3648.0
27	4841.0
28	6383.0
29	8896.0
30	11846.0
31	16393.0
32	23189.0
33	32868.0
34	71053.0
35	160034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.966930403726444	17.585647823811826	12.351129627670373	25.09629214479136
2	13.957983649823715	20.954703892531477	39.87235539186952	25.21495706577529
3	19.597964085554157	25.2822257806245	28.348678943154525	26.771131190666818
4	12.21945778997941	17.039865019446353	36.28460306566003	34.45607412491421
5	12.950827643611253	38.071606117726475	34.737004693841946	14.240561544820327
6	32.75071813593655	35.28221883976699	18.078428455349684	13.888634568946772
7	27.93530842994132	31.54143409188493	21.77529698010591	18.74796049806784
8	26.39641857349767	36.02636836711912	20.539961872921186	17.037251186462026
9	26.63029164901352	15.91588324878909	18.885905348616184	38.5679197535812
10	15.385760584510027	28.791756851158368	33.55693361276211	22.265548951569496
11	34.75498365030552	22.27395330458536	23.142978221405215	19.828084823703907
12	23.757089563990025	24.651212061348886	30.80241982816128	20.78927854649981
13	30.685390363308425	21.635889948180594	24.798305133270347	22.88041455524063
14	22.329060440724348	21.598813742721866	25.84117205883195	30.230953757721835
15	24.20922136075768	29.473253243852998	24.255603018816068	22.061922376573254
16	23.280499010966675	26.57479582401791	28.13119934961398	22.013505815401437
17	22.20806733274169	27.393432767455845	28.642752855629674	21.7557470441728
18	23.155619802773764	25.190284675607206	30.968526814467072	20.685568707151955
19	23.8859409613106	27.81212256509768	26.486127567988373	21.81580890560335
20	23.629125502272387	25.80850113905647	29.596579396242827	20.965793962428307
21	22.880456092248778	27.04212614087458	25.37413817282622	24.70327959405042
22	22.79196244561484	28.94120677810854	25.818067323105105	22.448763453171512
23	21.27886079282043	28.40978033173457	26.711329113199554	23.60002976224545
24	22.441223187327388	28.28670778300874	27.324814332527225	21.947254697136643
25	23.531011113114506	26.74044881756805	26.46169080756825	23.266849261749194
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	923.0
1	923.0
2	626.0
3	329.0
4	329.0
5	329.0
6	831.0
7	1333.0
8	1333.0
9	1333.0
10	1251.5
11	1170.0
12	1170.0
13	1170.0
14	1224.5
15	1279.0
16	1279.0
17	1279.0
18	2276.5
19	3274.0
20	3274.0
21	3274.0
22	5142.0
23	7010.0
24	7010.0
25	7010.0
26	10739.5
27	14469.0
28	14469.0
29	14469.0
30	18898.5
31	23328.0
32	23328.0
33	23328.0
34	25569.5
35	27811.0
36	27811.0
37	27811.0
38	30063.5
39	32316.0
40	32316.0
41	32316.0
42	36336.5
43	40357.0
44	40357.0
45	40357.0
46	50162.0
47	59967.0
48	59967.0
49	59967.0
50	51569.0
51	43171.0
52	43171.0
53	43171.0
54	37136.5
55	31102.0
56	31102.0
57	31102.0
58	27527.5
59	23953.0
60	23953.0
61	23953.0
62	20440.5
63	16928.0
64	16928.0
65	16928.0
66	13814.5
67	10701.0
68	10701.0
69	10701.0
70	8009.0
71	5317.0
72	5317.0
73	5317.0
74	3996.0
75	2675.0
76	2675.0
77	2675.0
78	1981.5
79	1288.0
80	1288.0
81	1288.0
82	934.0
83	580.0
84	580.0
85	580.0
86	404.0
87	228.0
88	228.0
89	228.0
90	148.5
91	69.0
92	69.0
93	69.0
94	47.0
95	25.0
96	25.0
97	25.0
98	72.0
99	119.0
100	119.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014297070244365525
2	2.859414048873105E-4
3	5.71882809774621E-4
4	0.01200953900526704
5	0.03288326156204071
6	0.05890392940678597
7	0.1063702026180795
8	0.10408267137898101
9	0.11380467914514956
10	0.125242335340642
11	0.13725187434590905
12	0.12610015955530393
13	0.12352668691131814
14	0.11094526509627647
15	0.1275298665797405
16	0.11180308931093841
17	0.11780785881357193
18	0.10779990964251604
19	0.0926450151834886
20	0.08864183551506626
21	0.09121530815905204
22	0.1035107885692064
23	0.0817792417977708
24	0.08778401130040432
25	0.09007154253950281
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	349722.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.89048440357814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.13727114319676	50.83515498640487
2	11.897878157824161	14.727308851249784
3	2.9691436674758065	5.512852195316826
4	1.045051295627172	2.587149236518105
5	0.5262804559795016	1.6285876176353669
6	0.30005456719484547	1.1142313506716983
7	0.17992538289411272	0.7794968372671137
8	0.1304559845181444	0.6459187260138919
9	0.10390262091571938	0.5787525185347699
>10	0.608919745097881	7.239223092781478
>50	0.051022328992409154	2.1432827126556266
>100	0.03989018448496708	4.929607653591904
>500	0.0064937509626690594	2.9634486261372714
>1k	0.0037107148358108914	4.314985595221285
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3822	1.0928680494793006	No Hit
TCCATGTACTCTGCGTTGATACCAC	3239	0.9261642104299987	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1657	0.47380490789827345	No Hit
CATGTACTCTGCGTTGATACCACTG	1459	0.41718850973058597	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1386	0.39631478717381236	No Hit
CCCATGTACTCTGCGTTGATACCAC	1186	0.33912650619635026	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1170	0.33455144371815326	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1112	0.3179668422346893	No Hit
CTGTAGGACGTGGAATATGGCAAGA	970	0.27736316274069117	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	917	0.2622082682816637	No Hit
TATCAACGCAGAGTACTTTTTTTTT	858	0.24533772539331242	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	834	0.23847513167601694	No Hit
GCGTTGATACCACTGCTTCCATGTA	831	0.237617307461355	No Hit
GTCCTACAGTGGACATTTCTAAATT	826	0.2361876004369185	No Hit
CATGGAAGCAGTGGTATCAACGCAG	816	0.23332818638804537	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	808	0.23104065514894687	No Hit
GTATCAACGCAGAGTACATGGGAAG	650	0.18586191317675183	No Hit
ACTCTGCGTTGATACCACTGCTTCC	605	0.17299454995682284	No Hit
CTTTAGGACGTGAAATATGGCGAGG	596	0.17042107731283707	No Hit
CAGTGGTATCAACGCAGAGTACATG	589	0.16841948747862587	No Hit
GGTATCAACGCAGAGTACTTTTTTT	521	0.14897547194628877	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	502	0.14354258525342986	No Hit
GTATCAACGCAGAGTACATGGAAGC	498	0.14239881963388062	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	466	0.1332486946774867	No Hit
GAGTACATGGGAAGCAGTGGTATCA	459	0.1312471048432755	No Hit
GTCCTACAGTGTGCATTTCTCATTT	431	0.12324074550643083	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	416	0.11895162443312116	No Hit
GGTATCAACGCAGAGTACATGGGAA	376	0.10751396823762875	No Hit
TATCAACGCAGAGTACATGGGAAGC	368	0.10522643699853026	No Hit
GCGTTGATACCACTGCTTCCCATGT	367	0.10494049559364295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.859414048873105E-4
2	0.0	0.0	0.0	0.0	2.859414048873105E-4
3	0.0	0.0	0.0	0.0	2.859414048873105E-4
4	0.0	0.0	0.0	0.0	2.859414048873105E-4
5	0.0	0.0	0.0	0.0	2.859414048873105E-4
6	0.0	0.0	0.0	0.0	5.71882809774621E-4
7	0.0	0.0	0.0	0.0	5.71882809774621E-4
8	0.0	0.0	0.0	0.0	5.71882809774621E-4
9	0.0	0.0	0.0	0.0	5.71882809774621E-4
10	0.0	0.0	0.0	2.859414048873105E-4	5.71882809774621E-4
11	0.0	0.0	0.0	2.859414048873105E-4	5.71882809774621E-4
12	0.0	0.0	0.0	2.859414048873105E-4	5.71882809774621E-4
13	0.0	0.0	0.0	2.859414048873105E-4	5.71882809774621E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACTCA	45	6.732964E-4	14.780899	4
GAAATGT	130	0.0	14.618471	6
ATAGGGT	40	0.0052608773	14.253009	3
TAGGGTC	40	0.0052608773	14.253009	4
TAGGACC	135	0.0	14.077047	4
CATAGGG	55	1.9569349E-4	13.815161	2
TGTCCAC	145	0.0	13.761527	10
AATGTCC	140	0.0	13.574295	8
TTAGAAA	135	1.8189894E-12	13.373195	3
CTACACT	50	0.0014931086	13.302809	4
GGACCGC	50	0.0014931086	13.302809	6
TCCAACG	50	0.0014979548	13.2970915	18
CTCTAGG	50	0.0015028146	13.291381	1
ATAGGAC	65	5.4181426E-5	13.156624	3
GTGTAGA	65	5.4620352E-5	13.145322	1
AAATGTC	150	1.8189894E-12	12.669342	7
TTTAGAA	160	0.0	12.466024	2
ATGTCCA	155	1.8189894E-12	12.2606535	9
GACAGTG	55	0.0030554184	12.093463	7
ACACCAT	55	0.0030554184	12.093463	6
>>END_MODULE