FastQCFastQC Report
Thu 2 Feb 2017
SRR4062332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349722
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6810.19472609672825844No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6190.17699772962524518No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC6140.17556802260080864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.15212082740004917No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA4590.1312471048432755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG250.00235070135.1994318
GTGTAAG454.0499464E-529.3454461
GTATTAA508.301439E-526.4109021
GCCGCGC450.001397525724.4405528
TTAGGAC3100.023.4156273
TAGGACA1600.023.3712774
TAGGACC1850.022.5909984
TGTAGGA4600.022.4778962
GGGTAGG803.5904995E-622.0090831
TAGGGAG602.8702166E-421.9964985
GTAGGAC5200.021.5734883
TAGGACG5200.021.5734884
AGGACGT5400.020.774475
CGAATGT856.0863003E-620.70850833
CTGTAGG5000.020.6885381
GATATAC1850.020.2245641
GTCCTAC4900.020.2124251
GCTTAGG550.00446666420.0082571
GACGTGA3300.019.9968157
CTAATAT550.00448148119.9968154
Produced by FastQC (version 0.11.5)