##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	349722
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.063530461337862	32.0	32.0	32.0	32.0	32.0
2	31.437238721041282	32.0	32.0	32.0	32.0	32.0
3	31.561980658923375	32.0	32.0	32.0	32.0	32.0
4	31.65252686419499	32.0	32.0	32.0	32.0	32.0
5	31.637534956336747	32.0	32.0	32.0	32.0	32.0
6	35.25978634458227	36.0	36.0	36.0	36.0	36.0
7	35.27587626743528	36.0	36.0	36.0	36.0	36.0
8	35.23644780711537	36.0	36.0	36.0	36.0	36.0
9	35.29421083031665	36.0	36.0	36.0	36.0	36.0
10	35.18546445462396	36.0	36.0	36.0	36.0	36.0
11	35.281234809362864	36.0	36.0	36.0	36.0	36.0
12	35.2074190356912	36.0	36.0	36.0	36.0	36.0
13	35.201868913022345	36.0	36.0	36.0	36.0	36.0
14	35.18142124315885	36.0	36.0	36.0	36.0	36.0
15	35.13641978485769	36.0	36.0	36.0	36.0	36.0
16	35.12450174710198	36.0	36.0	36.0	36.0	36.0
17	35.149058394953705	36.0	36.0	36.0	36.0	36.0
18	35.094363523026864	36.0	36.0	36.0	36.0	36.0
19	35.074976695775504	36.0	36.0	36.0	36.0	36.0
20	35.05255317080424	36.0	36.0	36.0	36.0	36.0
21	34.99050102652964	36.0	36.0	36.0	36.0	36.0
22	34.94675771040998	36.0	36.0	36.0	36.0	36.0
23	34.87487776004941	36.0	36.0	36.0	32.0	36.0
24	34.852065354767504	36.0	36.0	36.0	32.0	36.0
25	34.786567616564014	36.0	36.0	36.0	32.0	36.0
26	34.69529797953803	36.0	36.0	36.0	32.0	36.0
27	34.59148981190774	36.0	36.0	36.0	32.0	36.0
28	34.49977410629014	36.0	36.0	36.0	32.0	36.0
29	34.46409147837425	36.0	36.0	36.0	32.0	36.0
30	34.38668428065721	36.0	36.0	36.0	32.0	36.0
31	34.362510794288035	36.0	36.0	36.0	32.0	36.0
32	34.33159480959162	36.0	36.0	36.0	32.0	36.0
33	34.30280908836161	36.0	36.0	36.0	32.0	36.0
34	34.24369642172926	36.0	36.0	36.0	32.0	36.0
35	34.16111654399781	36.0	36.0	36.0	32.0	36.0
36	34.08746375692693	36.0	36.0	36.0	32.0	36.0
37	33.95311704725468	36.0	36.0	36.0	32.0	36.0
38	33.90571082173841	36.0	36.0	36.0	32.0	36.0
39	33.583526343781635	36.0	36.0	36.0	21.0	36.0
40	33.55857223737711	36.0	36.0	36.0	21.0	36.0
41	33.53948278918684	36.0	36.0	36.0	21.0	36.0
42	33.332727137554976	36.0	36.0	36.0	14.0	36.0
43	33.431216795054354	36.0	36.0	36.0	21.0	36.0
44	33.37007108503325	36.0	36.0	36.0	21.0	36.0
45	33.16103362099039	36.0	36.0	36.0	14.0	36.0
46	33.27307118225333	36.0	36.0	36.0	14.0	36.0
47	33.13916768175866	36.0	36.0	36.0	14.0	36.0
48	33.21093325555727	36.0	36.0	36.0	14.0	36.0
49	33.2288903757842	36.0	36.0	36.0	14.0	36.0
50	32.81738352176872	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	4.0
20	10.0
21	20.0
22	55.0
23	234.0
24	561.0
25	1431.0
26	2981.0
27	5289.0
28	7282.0
29	8965.0
30	11362.0
31	14624.0
32	20053.0
33	26703.0
34	55287.0
35	194860.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.085112468822224	18.48613304043386	12.692501315759364	25.73625317498455
2	13.510321615771097	21.261470447779974	39.66022596317447	25.567981973274463
3	19.149209943898295	25.388451398539413	28.854919049988276	26.607419607574016
4	12.018403188818546	17.582536986520722	36.216194577407194	34.18286524725354
5	12.766711845408638	38.16517119311911	34.876845036915036	14.191271924557219
6	33.05739987761708	35.39611462819039	17.778978731678304	13.767506762514225
7	27.94305190980264	31.481004912473338	21.873373708259702	18.70256946946432
8	26.455870662983745	36.23277917889066	20.3681781529329	16.943172005192697
9	26.56023927576761	15.656721624604685	19.017676897650134	38.765362201977574
10	15.444025380231672	28.67114070930828	33.63767117216295	22.2471627382971
11	35.03068151274441	21.914835211968363	23.281635127329707	19.772848147957518
12	23.706258113587364	24.609832953031265	30.892823442620138	20.791085490761233
13	31.013490715482583	21.22514454338017	25.00471803318064	22.756646707956605
14	22.28655903832187	21.476772979681	25.92745094675199	30.30921703524514
15	24.402525434487966	29.512584281229092	24.29758493889432	21.78730534538862
16	23.429667819020413	26.41377791884341	28.150600911017133	22.005953351119043
17	22.378918112100468	27.353154791520122	28.530661496846065	21.73726559953334
18	23.377425497966957	25.012152509707708	30.990043520281823	20.62037847204351
19	24.23875031811072	27.600287085991404	26.403618884768143	21.757343711129728
20	23.831854535472562	25.727365528521307	29.480643141246883	20.96013679475925
21	23.090626268864984	26.775553153647753	25.37872939077324	24.75509118671402
22	22.951372804684862	28.58327471534533	25.777331709186154	22.688020770783652
23	21.47191197579792	28.01339349540492	26.683480021274043	23.831214507523118
24	22.685577033055015	28.028136795150406	27.074516756262152	22.211769415532427
25	23.75086497274978	26.35521928846341	26.26857904278255	23.625336696004254
26	21.06748560987536	27.128351619443038	28.122292469096617	23.681870301584986
27	23.259178771588697	27.996111174654008	26.97300697701018	21.771703076747112
28	22.316457686973678	26.67200434639635	26.69916932359207	24.312368643037903
29	22.325924527978177	27.017119458765464	27.533248122063714	23.123707891192645
30	23.390913224121217	26.182599072964713	28.027805339746138	22.398682363167932
31	23.057588928285487	26.05084067253803	27.121983300926455	23.769587098250028
32	21.649729210392373	27.91134571283148	27.65799873040564	22.780926346370503
33	21.590129246254147	27.89917648404438	28.251744252544892	22.258950017156582
34	21.310598825339273	28.294797522575333	28.91644124694754	21.478162405137855
35	22.42981568217041	27.89101057411315	28.084021022412088	21.595152721304352
36	22.01240417361268	27.927976872993042	28.219351997735338	21.84026695565894
37	22.512303015918857	27.78923167987281	27.034322626397223	22.664142677811107
38	22.55734554875015	28.649613121279188	26.00293947764224	22.79010185232842
39	23.065243436911135	27.10646486770883	27.195678841133358	22.63261285424667
40	24.07147411793973	27.239999885623114	26.910308503063874	21.77821749337329
41	22.842118506911284	26.459318651027395	28.176416426949714	22.522146415111603
42	23.248984120538637	28.275049542889985	27.69740661200983	20.77855972456155
43	21.919429924394066	27.73542498312878	27.257597767280128	23.087547325197022
44	21.78391377826327	27.82218053605488	28.303160110150614	22.090745575531237
45	22.41644553232374	28.25038889143066	26.908141556480764	22.425024019764837
46	21.776651778310278	26.86023436293657	28.216771418930897	23.146342439822256
47	23.60145259064394	26.577261809447556	28.051012238362116	21.77027336154638
48	21.462212220083902	29.544499189312074	25.98748080514036	23.005807785463663
49	22.297204556892527	27.221542297662076	28.37334721141968	22.107905934025712
50	21.26645983157714	30.700141543828547	25.247987647084052	22.78541097751026
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	802.0
1	442.5
2	83.0
3	257.0
4	431.0
5	380.5
6	330.0
7	240.5
8	151.0
9	226.0
10	301.0
11	399.0
12	497.0
13	798.5
14	1100.0
15	1269.5
16	1439.0
17	1456.5
18	1474.0
19	1459.5
20	1445.0
21	1714.5
22	1984.0
23	2202.0
24	2420.0
25	2873.5
26	3327.0
27	4559.5
28	5792.0
29	6941.5
30	8091.0
31	9467.5
32	10844.0
33	13059.0
34	15274.0
35	17497.5
36	19721.0
37	20423.0
38	21125.0
39	20520.0
40	19915.0
41	20351.0
42	20787.0
43	21529.5
44	22272.0
45	23531.5
46	24791.0
47	25318.0
48	25845.0
49	24728.5
50	23612.0
51	22031.5
52	20451.0
53	19567.0
54	18683.0
55	18011.5
56	17340.0
57	16428.0
58	15516.0
59	14147.0
60	12778.0
61	11153.0
62	9528.0
63	8153.5
64	6779.0
65	5779.0
66	4779.0
67	4047.5
68	3316.0
69	2865.0
70	2414.0
71	1951.5
72	1489.0
73	1310.0
74	1131.0
75	887.0
76	643.0
77	554.0
78	465.0
79	359.5
80	254.0
81	203.5
82	153.0
83	117.0
84	81.0
85	64.0
86	47.0
87	32.0
88	17.0
89	9.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032597320157153394
2	0.005432886692858899
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.859414048873105E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.578242146619314E-4
17	0.0
18	0.0
19	2.859414048873105E-4
20	2.859414048873105E-4
21	0.0
22	0.0
23	0.0
24	5.71882809774621E-4
25	0.0
26	8.578242146619314E-4
27	5.71882809774621E-4
28	0.0020015898342111735
29	8.578242146619314E-4
30	0.0014297070244365525
31	5.71882809774621E-4
32	0.001143765619549242
33	5.71882809774621E-4
34	0.001143765619549242
35	0.0
36	2.859414048873105E-4
37	0.0031453554537604154
38	0.0
39	2.859414048873105E-4
40	2.859414048873105E-4
41	0.001143765619549242
42	0.007148535122182762
43	0.004003179668422347
44	0.005432886692858899
45	0.002859414048873105
46	0.002287531239098484
47	5.71882809774621E-4
48	0.005432886692858899
49	0.002859414048873105
50	0.0020015898342111735
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	349722.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.7737209033119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.41788079235494	59.872116943362265
2	11.202628519408298	16.08108665470987
3	2.7884224666357085	6.004063676425077
4	0.9747292906575824	2.7983979225576205
5	0.48468142350429744	1.739369460880868
6	0.2536928481823436	1.0925087808363485
7	0.17435236689386516	0.8759742684190481
8	0.11306161131076466	0.6491882028078044
9	0.0791325440636455	0.5111673418793813
>10	0.46229650672145967	6.071976911468158
>50	0.03354647920715606	1.6518923487625223
>100	0.013977699669192172	1.9511419777450012
>500	0.0015974513907648198	0.7011155101460662
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	681	0.19472609672825844	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	619	0.17699772962524518	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	614	0.17556802260080864	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	532	0.15212082740004917	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	459	0.1312471048432755	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	8.578242146619314E-4	0.0
11	0.0	0.0	0.0	8.578242146619314E-4	0.0
12	0.0	0.0	0.0	8.578242146619314E-4	0.0
13	0.0	0.0	0.0	8.578242146619314E-4	2.859414048873105E-4
14	0.0	0.0	0.0	0.0014297070244365525	2.859414048873105E-4
15	0.0	0.0	0.0	0.0014297070244365525	8.578242146619314E-4
16	0.0	0.0	0.0	0.0020015898342111735	8.578242146619314E-4
17	0.0	0.0	0.0	0.0025734726439857946	8.578242146619314E-4
18	0.0	0.0	0.0	0.0034312968586477257	8.578242146619314E-4
19	0.0	0.0	0.0	0.0037172382635350366	8.578242146619314E-4
20	0.0	0.0	0.0	0.005432886692858899	0.001143765619549242
21	0.0	0.0	0.0	0.007148535122182762	0.001143765619549242
22	0.0	0.0	0.0	0.010579831980830488	0.0014297070244365525
23	0.0	0.0	0.0	0.01572677726880208	0.0014297070244365525
24	0.0	0.0	0.0	0.02344719520075946	0.0014297070244365525
25	0.0	0.0	0.0	0.02945196470339298	0.0014297070244365525
26	0.0	0.0	0.0	0.04174744511354733	0.0014297070244365525
27	0.0	0.0	0.0	0.054328866928588994	0.0014297070244365525
28	0.0	0.0	0.0	0.0706275270071657	0.0014297070244365525
29	0.0	0.0	0.0	0.0846386558466439	0.0014297070244365525
30	0.0	0.0	0.0	0.10465455418875565	0.0014297070244365525
31	0.0	0.0	0.0	0.1306752220335009	0.0014297070244365525
32	0.0	0.0	0.0	0.16841948747862587	0.0014297070244365525
33	0.0	0.0	0.0	0.19787145218201885	0.0014297070244365525
34	0.0	0.0	0.0	0.23790324886624234	0.0014297070244365525
35	0.0	0.0	0.0	0.27135839323805766	0.0014297070244365525
36	0.0	0.0	0.0	0.32225596330799894	0.0014297070244365525
37	0.0	0.0	0.0	0.40346332229599513	0.0014297070244365525
38	0.0	0.0	0.0	0.4878160367377517	0.0014297070244365525
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	25	0.002350701	35.19943	18
GTGTAAG	45	4.0499464E-5	29.345446	1
GTATTAA	50	8.301439E-5	26.410902	1
GCCGCGC	45	0.0013975257	24.440552	8
TTAGGAC	310	0.0	23.415627	3
TAGGACA	160	0.0	23.371277	4
TAGGACC	185	0.0	22.590998	4
TGTAGGA	460	0.0	22.477896	2
GGGTAGG	80	3.5904995E-6	22.009083	1
TAGGGAG	60	2.8702166E-4	21.996498	5
GTAGGAC	520	0.0	21.573488	3
TAGGACG	520	0.0	21.573488	4
AGGACGT	540	0.0	20.77447	5
CGAATGT	85	6.0863003E-6	20.708508	33
CTGTAGG	500	0.0	20.688538	1
GATATAC	185	0.0	20.224564	1
GTCCTAC	490	0.0	20.212425	1
GCTTAGG	55	0.004466664	20.008257	1
GACGTGA	330	0.0	19.996815	7
CTAATAT	55	0.004481481	19.996815	4
>>END_MODULE