FastQCFastQC Report
Thu 2 Feb 2017
SRR4062331_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062331_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660499
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37860.22800375067976555No Hit
TATCAACGCAGAGTACTTTTTTTTT21740.13092449920174598No Hit
GCGCAAGACGGACCAGAGCGAAAGC21180.127552019001517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTC400.005278911414.2501583
TTACGGT400.00528313714.248442
GGACCGA604.0898198E-412.6675716
CGTTATT1501.8189894E-1212.665282
CGACCAT3250.012.57012810
GCGTTAT1600.012.4647541
CGGACCA4200.012.4421159
CGACCCG1405.2750693E-1112.2147895
CGGTCCA1900.012.00085710
GGTATCA9450.011.8586321
TCGCGTA1850.011.8123679
AAGACGG4750.011.8004875
CAAGACG4600.011.771874
CGCAAGA4500.011.6098392
TCTTATA1157.084418E-811.5639512
GACGGAC4450.011.52891357
CCTATTC1900.011.5001283
GTATCAA22500.011.4385561
ACGGTAT2250.011.4015019
ACGGACC4750.011.4008138
Produced by FastQC (version 0.11.5)