##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062331_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1660499
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200816742437063	32.0	32.0	32.0	32.0	32.0
2	30.736897161636353	32.0	32.0	32.0	32.0	32.0
3	30.742903187535795	32.0	32.0	32.0	32.0	32.0
4	30.740193158803468	32.0	32.0	32.0	32.0	32.0
5	30.65586971145421	32.0	32.0	32.0	32.0	32.0
6	34.3307680402096	36.0	36.0	36.0	32.0	36.0
7	34.24955269470201	36.0	36.0	36.0	32.0	36.0
8	34.18652164198834	36.0	36.0	36.0	32.0	36.0
9	34.33312757189255	36.0	36.0	36.0	32.0	36.0
10	33.99875820461199	36.0	36.0	36.0	32.0	36.0
11	34.33717454813282	36.0	36.0	36.0	32.0	36.0
12	34.12546047904877	36.0	36.0	36.0	32.0	36.0
13	34.23313955624183	36.0	36.0	36.0	32.0	36.0
14	34.12394828301613	36.0	36.0	36.0	32.0	36.0
15	34.072246354860795	36.0	36.0	36.0	32.0	36.0
16	34.07752067300251	36.0	36.0	36.0	32.0	36.0
17	34.00142969071345	36.0	36.0	36.0	32.0	36.0
18	34.005462815695765	36.0	36.0	36.0	32.0	36.0
19	34.01296718636988	36.0	36.0	36.0	32.0	36.0
20	34.005208072994925	36.0	36.0	36.0	32.0	36.0
21	33.97816379293212	36.0	36.0	36.0	32.0	36.0
22	33.94907735566236	36.0	36.0	36.0	32.0	36.0
23	33.91981747655373	36.0	36.0	36.0	32.0	36.0
24	33.897582594147906	36.0	36.0	36.0	32.0	36.0
25	33.52480248407256	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	23.0
5	146.0
6	351.0
7	87.0
8	288.0
9	219.0
10	118.0
11	36.0
12	62.0
13	71.0
14	314.0
15	394.0
16	651.0
17	951.0
18	1317.0
19	1803.0
20	2647.0
21	3962.0
22	5777.0
23	8509.0
24	11866.0
25	16543.0
26	21855.0
27	26678.0
28	34554.0
29	45229.0
30	57550.0
31	77573.0
32	108938.0
33	154482.0
34	336120.0
35	741383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.31552322220081	16.792614183850453	11.099079784158796	25.792782809789937
2	17.16066594599803	19.208669703913593	37.03660655406972	26.594057796018657
3	20.029967112058088	22.511708914312486	27.42833879458255	30.029985179046868
4	13.118987948643356	15.08289711987694	34.55876639978895	37.239348531690744
5	15.243270987668826	36.02403461813958	32.82578456196282	15.906909832228774
6	35.35719429511172	34.30857426605336	16.22266588486693	14.111565553967987
7	31.357355115998235	29.705930406483677	19.401286196508206	19.53542828100988
8	28.47145017255059	33.06484091824406	18.533754078632906	19.929954830572445
9	26.918072286250755	14.655566422384112	18.272109942826546	40.15425134853858
10	16.72310101678316	26.397225058178137	30.410988808209044	26.46868511682966
11	37.49008676605129	21.290038157281334	21.43339181261862	19.786483264048748
12	24.829369544740214	23.444907981971486	27.938890235680493	23.786832237607808
13	29.551940906007385	19.3225295846838	24.80138689982664	26.324142609482177
14	23.677191095375854	19.41004031222378	24.56103328562109	32.351735306779275
15	25.267297285236506	26.894901059299293	21.835459449810827	26.002342205653374
16	26.04337316271237	25.81901258433118	23.179263559464598	24.958350693491855
17	24.18598481079826	26.031059776955708	24.616456074022487	25.166499338223545
18	24.878739627678655	25.09488303837947	25.552733264561343	24.473644069380526
19	25.551545388069606	25.014362454704763	24.926590223644368	24.507501933581256
20	25.890984187607597	24.32589972464544	24.54314531101563	25.239970776731337
21	26.503649265445183	24.181416836049486	24.38654485570513	24.928389042800205
22	26.190272368849392	24.145576869747646	24.785505009705926	24.878645751697032
23	24.626051086210314	24.44215808770569	25.23330181054139	25.698489015542602
24	24.767867734639847	24.825132250893326	25.21615847924734	25.190841535219487
25	25.00433998476183	24.42290258180871	25.076190843596596	25.496566589832863
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	582.0
1	582.0
2	652.0
3	722.0
4	722.0
5	722.0
6	1403.0
7	2084.0
8	2084.0
9	2084.0
10	2418.0
11	2752.0
12	2752.0
13	2752.0
14	3444.0
15	4136.0
16	4136.0
17	4136.0
18	6716.0
19	9296.0
20	9296.0
21	9296.0
22	14550.5
23	19805.0
24	19805.0
25	19805.0
26	29461.0
27	39117.0
28	39117.0
29	39117.0
30	50476.0
31	61835.0
32	61835.0
33	61835.0
34	76765.5
35	91696.0
36	91696.0
37	91696.0
38	109217.5
39	126739.0
40	126739.0
41	126739.0
42	147952.5
43	169166.0
44	169166.0
45	169166.0
46	191157.0
47	213148.0
48	213148.0
49	213148.0
50	223095.5
51	233043.0
52	233043.0
53	233043.0
54	219688.5
55	206334.0
56	206334.0
57	206334.0
58	191613.0
59	176892.0
60	176892.0
61	176892.0
62	155323.5
63	133755.0
64	133755.0
65	133755.0
66	108911.0
67	84067.0
68	84067.0
69	84067.0
70	63522.0
71	42977.0
72	42977.0
73	42977.0
74	32290.5
75	21604.0
76	21604.0
77	21604.0
78	16864.5
79	12125.0
80	12125.0
81	12125.0
82	8483.5
83	4842.0
84	4842.0
85	4842.0
86	3522.5
87	2203.0
88	2203.0
89	2203.0
90	1453.0
91	703.0
92	703.0
93	703.0
94	431.5
95	160.0
96	160.0
97	160.0
98	438.0
99	716.0
100	716.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001144234353649114
2	2.4089144287349766E-4
3	7.22674328620493E-4
4	0.011321897815054391
5	0.033724802002289674
6	0.06504068957584437
7	0.10900337790025769
8	0.11171340663258456
9	0.12267396728332869
10	0.13429697940197496
11	0.14224639701680036
12	0.13471853942700357
13	0.12791335616582727
14	0.11713346409723824
15	0.1344776479841301
16	0.12092750432249583
17	0.1255044417370923
18	0.11309853242910715
19	0.09996994879250153
20	0.09316476553132522
21	0.09244209120270473
22	0.1035230975748856
23	0.08322799351279345
24	0.09220119975983124
25	0.09075585110259024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1660499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.60795447905172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57622246489821	37.9711833970905
2	15.075929113364925	15.560757268238953
3	5.028017879675382	7.784571535624357
4	2.158019098451737	4.45483805591286
5	1.1618654397286874	2.998074936215076
6	0.6686072880016612	2.070327269013118
7	0.4540849773978748	1.640407779021953
8	0.30743864758815626	1.2693023783864634
9	0.23642894417309906	1.0981452769574003
>10	1.1802275081440912	10.851271131549918
>50	0.07763765673462697	2.768861637057408
>100	0.06540477800634827	6.903625444829552
>500	0.007645968015045648	2.672667886127152
>1k	0.002470235820245517	1.9559660039753057
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3786	0.22800375067976555	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2174	0.13092449920174598	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2118	0.127552019001517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2044572143674883E-4
2	0.0	0.0	0.0	0.0	1.2044572143674883E-4
3	0.0	0.0	0.0	0.0	1.2044572143674883E-4
4	0.0	0.0	0.0	0.0	1.2044572143674883E-4
5	0.0	0.0	0.0	0.0	1.8066858215512325E-4
6	0.0	0.0	0.0	0.0	2.4089144287349766E-4
7	0.0	0.0	0.0	0.0	2.4089144287349766E-4
8	0.0	0.0	0.0	0.0	2.4089144287349766E-4
9	0.0	0.0	0.0	0.0	2.4089144287349766E-4
10	0.0	0.0	0.0	0.0	3.011143035918721E-4
11	0.0	0.0	0.0	0.0	3.011143035918721E-4
12	0.0	0.0	0.0	0.0	7.828971893388675E-4
13	0.0	0.0	0.0	0.0	8.431200500572418E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTC	40	0.0052789114	14.250158	3
TTACGGT	40	0.005283137	14.24844	2
GGACCGA	60	4.0898198E-4	12.667571	6
CGTTATT	150	1.8189894E-12	12.66528	2
CGACCAT	325	0.0	12.570128	10
GCGTTAT	160	0.0	12.464754	1
CGGACCA	420	0.0	12.442115	9
CGACCCG	140	5.2750693E-11	12.214789	5
CGGTCCA	190	0.0	12.000857	10
GGTATCA	945	0.0	11.858632	1
TCGCGTA	185	0.0	11.812367	9
AAGACGG	475	0.0	11.800487	5
CAAGACG	460	0.0	11.77187	4
CGCAAGA	450	0.0	11.609839	2
TCTTATA	115	7.084418E-8	11.563951	2
GACGGAC	445	0.0	11.5289135	7
CCTATTC	190	0.0	11.500128	3
GTATCAA	2250	0.0	11.438556	1
ACGGTAT	225	0.0	11.401501	9
ACGGACC	475	0.0	11.400813	8
>>END_MODULE