FastQCFastQC Report
Thu 2 Feb 2017
SRR4062331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660499
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20570.12387842449769618No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16730.1007528459818404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23850.026.5762211
CGCGATT450.001398745924.44350612
GGTATCA10450.024.0092031
CGTTCGC808.993792E-519.24926215
GTTCGCG708.127162E-418.8564216
TCAACGC34650.018.3485034
ATCAACG34800.018.3326323
GGCGTTA5150.017.9437642
AACGCAG35700.017.8088426
TATCAAC36250.017.7829932
CAACGCA35950.017.6849965
CGTTATT5150.017.08929644
GTGTAGG3050.016.5965251
GCGTTAT5350.016.45044543
ACTGCGC1351.0864787E-616.2956718
ATACCGT5050.016.1181956
GATTTCG1958.913048E-1015.79664541
TGCACCG1958.931238E-1015.7942675
CGGCGTT5750.015.68871841
ATTTCGT2152.1464075E-1015.35055942
Produced by FastQC (version 0.11.5)