##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062331_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1660499
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.162128372254365	32.0	32.0	32.0	32.0	32.0
2	31.460137585147596	32.0	32.0	32.0	32.0	32.0
3	31.570032261386487	32.0	32.0	32.0	32.0	32.0
4	31.619128346358533	32.0	32.0	32.0	32.0	32.0
5	31.635354793950494	32.0	32.0	32.0	32.0	32.0
6	35.231178097668234	36.0	36.0	36.0	36.0	36.0
7	35.23754847187502	36.0	36.0	36.0	36.0	36.0
8	35.18495343869524	36.0	36.0	36.0	36.0	36.0
9	35.28991586264129	36.0	36.0	36.0	36.0	36.0
10	35.165974806368446	36.0	36.0	36.0	36.0	36.0
11	35.288514476672376	36.0	36.0	36.0	36.0	36.0
12	35.22201699609575	36.0	36.0	36.0	36.0	36.0
13	35.2384186922124	36.0	36.0	36.0	36.0	36.0
14	35.180269906817166	36.0	36.0	36.0	36.0	36.0
15	35.1614857943305	36.0	36.0	36.0	36.0	36.0
16	35.207070886522665	36.0	36.0	36.0	36.0	36.0
17	35.190657145833875	36.0	36.0	36.0	36.0	36.0
18	35.16806755077841	36.0	36.0	36.0	36.0	36.0
19	35.17407478113507	36.0	36.0	36.0	36.0	36.0
20	35.16530573038587	36.0	36.0	36.0	36.0	36.0
21	35.16660714640599	36.0	36.0	36.0	36.0	36.0
22	35.14096545676932	36.0	36.0	36.0	36.0	36.0
23	35.08844509993683	36.0	36.0	36.0	36.0	36.0
24	35.1069780830943	36.0	36.0	36.0	36.0	36.0
25	35.07496180365059	36.0	36.0	36.0	36.0	36.0
26	35.04507801570492	36.0	36.0	36.0	36.0	36.0
27	35.02742368408533	36.0	36.0	36.0	36.0	36.0
28	35.007733217544846	36.0	36.0	36.0	36.0	36.0
29	34.966866586489964	36.0	36.0	36.0	36.0	36.0
30	34.97072024734733	36.0	36.0	36.0	36.0	36.0
31	34.95417461859357	36.0	36.0	36.0	36.0	36.0
32	34.90366148970882	36.0	36.0	36.0	32.0	36.0
33	34.856423882218536	36.0	36.0	36.0	32.0	36.0
34	34.839434410981276	36.0	36.0	36.0	32.0	36.0
35	34.825200135621884	36.0	36.0	36.0	32.0	36.0
36	34.80124829945697	36.0	36.0	36.0	32.0	36.0
37	34.80528262889649	36.0	36.0	36.0	32.0	36.0
38	34.801324180261474	36.0	36.0	36.0	32.0	36.0
39	34.78824558159926	36.0	36.0	36.0	32.0	36.0
40	34.78991435706977	36.0	36.0	36.0	32.0	36.0
41	34.78292970968366	36.0	36.0	36.0	32.0	36.0
42	34.761737887225465	36.0	36.0	36.0	32.0	36.0
43	34.75395769584926	36.0	36.0	36.0	32.0	36.0
44	34.705380129708	36.0	36.0	36.0	32.0	36.0
45	34.687109718223255	36.0	36.0	36.0	32.0	36.0
46	34.66006182478881	36.0	36.0	36.0	32.0	36.0
47	34.632709203679134	36.0	36.0	36.0	32.0	36.0
48	34.60407082449312	36.0	36.0	36.0	32.0	36.0
49	34.58262907716295	36.0	36.0	36.0	32.0	36.0
50	34.182269908021624	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	23.0
21	73.0
22	189.0
23	505.0
24	1147.0
25	2533.0
26	5070.0
27	9059.0
28	14874.0
29	22454.0
30	32417.0
31	46697.0
32	68119.0
33	113454.0
34	257202.0
35	1086675.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.725719586982976	17.439366739358306	11.543874022578585	26.291039651080133
2	16.60610988150624	19.592092385985517	36.74468885977988	27.05710887272837
3	19.45781227886865	22.69720776033653	27.87415800710029	29.970821953694532
4	12.793993127362995	15.438982763002423	34.65851810721844	37.10850600241614
5	14.862279350966185	36.31426456745834	32.81230521668486	16.011150864890613
6	35.64511107216695	34.32809574482821	16.119511206584537	13.907281976420313
7	31.321909859626533	29.70817808381697	19.530153285247387	19.43975877130911
8	28.571050027732625	33.01585848591297	18.573392697014572	19.83969878933983
9	27.061082240940827	14.310336832482285	18.254994432396526	40.373586494180365
10	16.665191204801935	26.32645527212636	30.52808887941381	26.48026464365789
11	37.828989960246886	21.1122680591798	21.39459283022754	19.664149150345768
12	24.865070599302136	23.271151184765053	28.06597779702234	23.797800418910473
13	29.626214770379267	19.150207256975165	24.977130368642197	26.246447604003375
14	23.683181983247202	19.2103096719721	24.66505550439958	32.44145284038111
15	25.3409366702419	26.78453886452205	21.92852871335665	25.9459957518794
16	26.01744063499328	25.695065914278313	23.28216369866726	25.00532975206114
17	24.35611222891432	25.815793927006283	24.668608653181963	25.159485190897435
18	25.000873232006306	24.86308324370159	25.684523558595075	24.451519965697038
19	25.628982613057882	24.663670378603058	25.200557181907367	24.506789826431692
20	25.99467147807465	23.985394743022876	24.880367215136665	25.139566563765808
21	26.654654206147793	23.956367306675467	24.52902683411844	24.8599516530583
22	26.23536659763119	23.929915043610382	24.97038540824174	24.864332950516683
23	24.715642707403017	24.145994667867914	25.468187574939822	25.67017504978925
24	24.84971267448205	24.455912305192577	25.501931362263818	25.192443658061553
25	25.185848083946	24.016980468486523	25.350859020437266	25.44631242713021
26	24.708851001812718	25.080548512788393	25.61436684352209	24.596233641876793
27	25.282310735426165	24.47014230111178	25.10013592348778	25.147411039974273
28	24.88024532694802	24.404117368613136	25.501994004136158	25.213643300302685
29	24.658369120940733	24.69974242558376	25.48354673406842	25.15834171940709
30	24.61154421750272	24.851052553922496	25.589330828041206	24.94807240053358
31	25.270839217651208	24.571164965458927	24.826511740230426	25.33148407665944
32	25.257529248878797	24.600012887754286	24.7553283938305	25.38712946953642
33	24.67914688089997	24.3815850092894	25.35719770309456	25.582070406716067
34	25.323594045375735	24.620850097952953	25.316487713573878	24.739068143097434
35	25.652515874774767	24.252873840105607	25.468594925853477	24.626015359266145
36	24.48133029970527	24.929990882253396	25.193526279465555	25.395152538575775
37	25.488322065520734	24.667235176292184	24.98093926944104	24.863503488746037
38	24.79513688355127	24.52497713036864	25.260358482600715	25.41952750347938
39	25.586680999828364	24.41431268575145	24.74963640141284	25.249369913007346
40	25.66537267735342	24.507467036997337	25.139024557692935	24.68813572795631
41	24.680665780982196	25.039958180918116	25.452367559858978	24.827008478240707
42	25.709729213416832	24.99533251185542	24.979131811069717	24.315806463658035
43	25.335333611172008	24.22496260018453	25.270651804706716	25.169051983936747
44	24.748936877536604	24.794948805707406	25.01850414257916	25.43761017417683
45	24.995603649091702	24.987172291185377	24.973320774624984	25.043903285097933
46	24.836205323454962	24.738943294731648	24.957557018735134	25.467294363078253
47	25.058913723088338	24.622955183413943	25.281198979330664	25.036932114167055
48	25.44922260820128	25.179532456770076	24.445984880970162	24.92526005405848
49	24.986012650947437	25.20312521643503	24.53173246191195	25.27912967070558
50	25.205378968687725	25.32498387501829	24.52930410541131	24.940333050882675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	338.0
1	220.5
2	103.0
3	233.5
4	364.0
5	368.0
6	372.0
7	334.5
8	297.0
9	389.5
10	482.0
11	776.5
12	1071.0
13	1898.5
14	2726.0
15	3515.0
16	4304.0
17	4175.0
18	4046.0
19	3897.5
20	3749.0
21	4113.0
22	4477.0
23	5069.5
24	5662.0
25	7013.0
26	8364.0
27	10478.5
28	12593.0
29	15211.0
30	17829.0
31	20667.0
32	23505.0
33	26933.0
34	30361.0
35	35372.5
36	40384.0
37	47576.0
38	54768.0
39	60568.0
40	66368.0
41	72847.0
42	79326.0
43	84211.0
44	89096.0
45	97216.0
46	105336.0
47	112502.0
48	119668.0
49	126256.5
50	132845.0
51	130827.5
52	128810.0
53	128167.0
54	127524.0
55	128179.0
56	128834.0
57	124825.5
58	120817.0
59	111141.0
60	101465.0
61	88731.5
62	75998.0
63	65173.5
64	54349.0
65	46050.5
66	37752.0
67	32291.0
68	26830.0
69	23477.0
70	20124.0
71	15441.0
72	10758.0
73	9198.0
74	7638.0
75	5646.5
76	3655.0
77	3223.5
78	2792.0
79	2373.5
80	1955.0
81	1563.5
82	1172.0
83	999.5
84	827.0
85	663.0
86	499.0
87	326.5
88	154.0
89	101.5
90	49.0
91	34.5
92	20.0
93	17.5
94	15.0
95	15.5
96	16.0
97	12.0
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03125566471283632
2	0.004456491693159707
3	2.4089144287349766E-4
4	6.0222860718374416E-5
5	0.0
6	1.2044572143674883E-4
7	0.0
8	0.0
9	0.0
10	1.2044572143674883E-4
11	0.0
12	6.0222860718374416E-5
13	0.0
14	0.0
15	0.0
16	5.420057464653698E-4
17	0.0
18	6.0222860718374416E-5
19	0.0
20	6.0222860718374416E-5
21	6.0222860718374416E-5
22	0.0
23	0.0
24	7.828971893388675E-4
25	1.8066858215512325E-4
26	5.420057464653698E-4
27	3.613371643102465E-4
28	0.0012646800750858629
29	4.817828857469953E-4
30	8.431200500572417E-4
31	7.22674328620493E-4
32	4.817828857469953E-4
33	2.4089144287349766E-4
34	4.817828857469953E-4
35	1.8066858215512325E-4
36	6.0222860718374416E-5
37	0.001023788632212365
38	0.0
39	8.431200500572417E-4
40	6.0222860718374416E-5
41	7.828971893388675E-4
42	0.00463716027531483
43	0.003794040225257588
44	0.0036133716431024647
45	0.001866908682269607
46	0.002168022985861479
47	0.0012044572143674883
48	0.004516714553878081
49	0.004215600250286209
50	0.0019271315429879813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1660499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.84799311937629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.96729393229685	38.56175047673532
2	15.558173729983155	16.444365164644214
3	5.226092850026868	8.285655569383247
4	2.2088714555351623	4.669376939348357
5	1.134044804209663	2.996599600496835
6	0.6844942274815309	2.1704487734518043
7	0.4356680807243437	1.611692861271639
8	0.31271425238402534	1.3221056526657498
9	0.22230093231830395	1.0573342327429796
>10	1.1134680315582124	10.367566198695782
>50	0.07137706287009357	2.62844492032459
>100	0.05768687596349659	6.237533515331931
>500	0.005745415182555842	2.101144941286845
>1k	0.0020683494657201032	1.5459811536208237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2057	0.12387842449769618	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1673	0.1007528459818404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8066858215512325E-4
2	0.0	0.0	0.0	0.0	1.8066858215512325E-4
3	0.0	0.0	0.0	0.0	1.8066858215512325E-4
4	0.0	0.0	0.0	0.0	1.8066858215512325E-4
5	0.0	0.0	0.0	0.0	1.8066858215512325E-4
6	0.0	0.0	0.0	0.0	1.8066858215512325E-4
7	0.0	0.0	0.0	0.0	1.8066858215512325E-4
8	0.0	0.0	0.0	0.0	1.8066858215512325E-4
9	0.0	0.0	0.0	0.0	1.8066858215512325E-4
10	0.0	0.0	0.0	0.0	1.8066858215512325E-4
11	0.0	0.0	0.0	0.0	1.8066858215512325E-4
12	0.0	0.0	0.0	0.0	0.001023788632212365
13	0.0	0.0	0.0	0.0	0.001023788632212365
14	0.0	0.0	0.0	0.0	0.001023788632212365
15	0.0	0.0	0.0	0.0	0.0012646800750858627
16	0.0	0.0	0.0	6.0222860718374416E-5	0.0013851257965226116
17	0.0	0.0	0.0	1.8066858215512325E-4	0.001445348657240986
18	0.0	0.0	0.0	3.613371643102465E-4	0.001445348657240986
19	0.0	0.0	0.0	4.215600250286209E-4	0.001445348657240986
20	0.0	0.0	0.0	6.022286071837442E-4	0.0015055715179593603
21	0.0	0.0	0.0	7.828971893388675E-4	0.0015055715179593603
22	0.0	0.0	0.0	8.431200500572418E-4	0.001565794378677735
23	0.0	0.0	0.0	0.0012646800750858627	0.001565794378677735
24	0.0	0.0	0.0	0.00204757726442473	0.0016260172393961093
25	0.0	0.0	0.0	0.0025895830108901	0.0016260172393961093
26	0.0	0.0	0.0	0.0030111430359187207	0.00204757726442473
27	0.0	0.0	0.0	0.0036735945038208393	0.00204757726442473
28	0.0	0.0	0.0	0.004576937414596456	0.00204757726442473
29	0.0	0.0	0.0	0.007708526171951925	0.00204757726442473
30	0.0	0.0	0.0	0.01336947507947912	0.00204757726442473
31	0.0	0.0	0.0	0.024390258590941637	0.00204757726442473
32	0.0	0.0	0.0	0.03498948207737554	0.00204757726442473
33	0.0	0.0	0.0	0.04769650568895254	0.00204757726442473
34	0.0	0.0	0.0	0.05823550631466806	0.0021078001251431046
35	0.0	0.0	0.0	0.07166520425486556	0.0021078001251431046
36	0.0	0.0	0.0	0.08906961100247576	0.0021078001251431046
37	0.0	0.0	0.0	0.11755502412226686	0.0021078001251431046
38	0.0	0.0	0.0	0.15447163774263037	0.0022282458465798533
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2385	0.0	26.576221	1
CGCGATT	45	0.0013987459	24.443506	12
GGTATCA	1045	0.0	24.009203	1
CGTTCGC	80	8.993792E-5	19.249262	15
GTTCGCG	70	8.127162E-4	18.85642	16
TCAACGC	3465	0.0	18.348503	4
ATCAACG	3480	0.0	18.332632	3
GGCGTTA	515	0.0	17.94376	42
AACGCAG	3570	0.0	17.808842	6
TATCAAC	3625	0.0	17.782993	2
CAACGCA	3595	0.0	17.684996	5
CGTTATT	515	0.0	17.089296	44
GTGTAGG	305	0.0	16.596525	1
GCGTTAT	535	0.0	16.450445	43
ACTGCGC	135	1.0864787E-6	16.295671	8
ATACCGT	505	0.0	16.118195	6
GATTTCG	195	8.913048E-10	15.796645	41
TGCACCG	195	8.931238E-10	15.794267	5
CGGCGTT	575	0.0	15.688718	41
ATTTCGT	215	2.1464075E-10	15.350559	42
>>END_MODULE