FastQCFastQC Report
Thu 2 Feb 2017
SRR4062330_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062330_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080122
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23720.2196048224182083No Hit
TATCAACGCAGAGTACTTTTTTTTT14930.13822512642090432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT402.7648135E-416.621312
TCGGCGT508.7058346E-515.20085313
ACCGTGC456.754705E-414.7792929
CGCATCG1058.913048E-1114.47700313
GCATCGC1101.8553692E-1013.81895714
GCGTTAT551.9652865E-413.8131941
CGGTCCA1153.765308E-1013.21813310
CGTCAAT1153.765308E-1013.21690719
GCGGTCG655.4442356E-513.1551939
CGGTCGG655.4466043E-513.15458410
GCGTCCC655.448974E-513.15397516
GGACCAT751.4760337E-512.6679656
CGCCAGT1207.385097E-1012.66737718
GTATCAA14100.012.6620941
ACCCGGT853.937683E-612.29480712
GGTCGGC701.0897327E-412.21497211
GTTATAC701.0920932E-412.212143
GCTCACG550.003066109712.09158811
GGTATCA4950.012.0865451
CATCGCC1353.7107384E-1011.963634515
Produced by FastQC (version 0.11.5)