FastQCFastQC Report
Thu 2 Feb 2017
SRR4062330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080122
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG852.6375346E-1028.4697195
GTATCAA16600.023.0638081
GGTATCA8200.020.9300671
CGCAATA1601.8189894E-1220.62532636
TAGACGG550.004484099319.999395
TAGCGGC1800.019.55495830
CTAGCGG1705.456968E-1219.41117329
TTAGGAC1950.019.1789023
ATACCGT2000.018.699436
AGTTCGC600.00740697318.3353241
TAGAGCG600.00741290918.3327737
ATTACAC1951.8189894E-1218.0507323
TAGGACG1102.7966798E-617.999454
AACGCAG21200.017.53726
AATACGA1903.274181E-1117.37030439
TCTAGCG1903.274181E-1117.36789128
CGTCGTA1903.274181E-1117.36789110
TTACACC1307.317358E-716.922564
ATCAACG22150.016.8843613
TACCGTC2109.094947E-1216.7613937
Produced by FastQC (version 0.11.5)