FastQCFastQC Report
Thu 2 Feb 2017
SRR4062329_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062329_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225228
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24571.0908945601790185No Hit
TATCAACGCAGAGTACTTTTTTTTT15680.6961834230202284No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14230.6318042161720567No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9500.42179480348802106No Hit
GGTATCAACGCAGAGTACTTTTTTT9270.41158292929831103No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7950.3529756513399755No Hit
GTACATGGGAAGCAGTGGTATCAAC7780.34542774433018986No Hit
CCCATGTACTCTGCGTTGATACCAC5540.2459729696130144No Hit
GTATCAACGCAGAGTACATGGGAAG5330.23664908448327915No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4370.19402560960448967No Hit
GTCCTAAAGTGTGTATTTCTCATTT3880.17226987763510754No Hit
GTACATGGGAGTGGTATCAACGCAA3140.13941428241604062No Hit
CTGTAGGACGTGGAATATGGCAAGA3110.13808229882607848No Hit
TATCAACGCAGAGTACATGGGAAGC2990.13275436446622976No Hit
GTATCAACGCAGAGTACATGGGAGT2940.13053439181629284No Hit
GAGTACATGGGAAGCAGTGGTATCA2870.12742643010638108No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2860.1269824355763937No Hit
GTCCTACAGTGGACATTTCTAAATT2670.11854653950663328No Hit
GCGTTGATACCACTGCTTCCCATGT2580.11455058873674677No Hit
ATCAACGCAGAGTACTTTTTTTTTT2450.10877865984691068No Hit
GGTATCAACGCAGAGTACATGGGAA2360.10478270907702417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATGG250.00602372618.9959983
TACCCTG350.002162277216.2859045
TTGGATG350.002162277216.2859044
GTCCTAG350.00216549316.2822841
GGGACGC551.9482514E-413.8183427
GTCCTAA1301.4551915E-1113.1510751
GACGCAG604.0598592E-412.6696339
GGACGCA604.0674896E-412.6668168
AAAGTGT1103.7483915E-812.091056
ACATTTC802.8340988E-511.87778113
ATGGCAA1052.6873386E-711.75942717
TAGGACA657.9759106E-411.6924454
TGGCAAG1156.952905E-811.56535318
GTATTTC1251.7900675E-811.40266913
CCTAAAG1251.8018909E-811.3975983
TCCTAAA1354.640242E-911.2568872
TTAGGAC1104.9090704E-711.2249073
TGGACAG600.005848704411.0834645
AGGACAT600.005848704411.0834645
CTAAAGT1303.2026946E-810.9616674
Produced by FastQC (version 0.11.5)