##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062329_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225228
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01270712344824	32.0	32.0	32.0	32.0	32.0
2	30.830935762871402	32.0	32.0	32.0	32.0	32.0
3	30.850520361589144	32.0	32.0	32.0	32.0	32.0
4	30.777532100804518	32.0	32.0	32.0	32.0	32.0
5	30.85709148063296	32.0	32.0	32.0	32.0	32.0
6	34.39894684497487	36.0	36.0	36.0	32.0	36.0
7	34.30447368888415	36.0	36.0	36.0	32.0	36.0
8	34.23670680377218	36.0	36.0	36.0	32.0	36.0
9	34.21658941161845	36.0	36.0	36.0	32.0	36.0
10	34.16606727405119	36.0	36.0	36.0	32.0	36.0
11	34.35135507130552	36.0	36.0	36.0	32.0	36.0
12	34.255141456657256	36.0	36.0	36.0	32.0	36.0
13	34.23862486014173	36.0	36.0	36.0	32.0	36.0
14	34.19220967197684	36.0	36.0	36.0	32.0	36.0
15	34.1901628571936	36.0	36.0	36.0	32.0	36.0
16	34.17751345303426	36.0	36.0	36.0	32.0	36.0
17	34.12182765908324	36.0	36.0	36.0	32.0	36.0
18	34.11565613511642	36.0	36.0	36.0	32.0	36.0
19	34.0986955440709	36.0	36.0	36.0	32.0	36.0
20	34.04082529703234	36.0	36.0	36.0	32.0	36.0
21	34.00666879784041	36.0	36.0	36.0	32.0	36.0
22	33.95816683538459	36.0	36.0	36.0	32.0	36.0
23	33.96508427016179	36.0	36.0	36.0	32.0	36.0
24	33.929138473014014	36.0	36.0	36.0	32.0	36.0
25	33.502002415330246	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	19.0
6	55.0
7	8.0
8	31.0
9	38.0
10	15.0
11	3.0
12	12.0
13	13.0
14	95.0
15	142.0
16	182.0
17	276.0
18	337.0
19	471.0
20	559.0
21	746.0
22	956.0
23	1215.0
24	1595.0
25	1926.0
26	2504.0
27	3067.0
28	4039.0
29	5288.0
30	6847.0
31	9523.0
32	13722.0
33	19722.0
34	45183.0
35	106634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.523935957660306	17.172972924973138	11.500892436930018	24.80219868043654
2	15.027505583256003	20.874051512474082	39.30834225026307	24.790100654006846
3	19.24777778764441	26.283821583653754	28.213882944242673	26.254517684459167
4	12.23612578929139	16.05491958330743	36.25722684523228	35.451727782168895
5	12.940162740290658	38.102281206694386	33.98047471840244	14.977081334612516
6	32.14845815173689	36.023848556336	17.756689635563138	14.071003656363985
7	27.70181973348505	31.668429802025088	21.904808470161527	18.724941994328333
8	27.037791033630555	33.68433685670344	20.16588583569657	19.111986273969436
9	27.628391203652253	14.185822179349831	19.353920971563454	38.83186564543446
10	15.165055018339446	28.34856063132155	33.158608425030565	23.327775925308437
11	34.64428634948866	22.45620275678079	23.277456647398846	19.622054246331704
12	24.76180737407913	24.664440719712967	30.49843724296759	20.07531466324031
13	30.119110605245492	21.28340676782991	25.23197446169031	23.365508165234285
14	22.794820389493196	20.494845727443668	27.784618530487776	28.92571535257536
15	24.095523078222747	28.004659662352328	24.88562040647551	23.014196852949414
16	22.426416654441674	27.11325678264851	27.22572672027882	23.234599842630995
17	21.865038966119705	26.73880474261911	28.100062683660905	23.296093607600284
18	22.08146083647824	25.362830992990084	30.577373570345873	21.978334600185807
19	23.686666666666667	25.404444444444447	29.284444444444446	21.624444444444443
20	24.244269245469173	25.019332130445214	29.284400081772688	21.45199854231292
21	23.63105475780969	25.018110386695643	28.559746499028925	22.791088356465742
22	23.682023245994177	25.9523968264551	27.908527235149013	22.457052692401717
23	21.895413219100774	26.486637812280595	29.025320168149378	22.592628800469253
24	22.678428584125857	26.104790685272423	29.21295884810239	22.003821882499334
25	22.32186901434114	25.457632090019867	29.547100885710858	22.67339800992814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	372.0
1	372.0
2	397.0
3	422.0
4	422.0
5	422.0
6	1108.5
7	1795.0
8	1795.0
9	1795.0
10	1698.5
11	1602.0
12	1602.0
13	1602.0
14	1353.0
15	1104.0
16	1104.0
17	1104.0
18	1836.0
19	2568.0
20	2568.0
21	2568.0
22	3724.5
23	4881.0
24	4881.0
25	4881.0
26	7066.0
27	9251.0
28	9251.0
29	9251.0
30	11654.0
31	14057.0
32	14057.0
33	14057.0
34	14981.5
35	15906.0
36	15906.0
37	15906.0
38	17622.0
39	19338.0
40	19338.0
41	19338.0
42	21709.5
43	24081.0
44	24081.0
45	24081.0
46	28048.5
47	32016.0
48	32016.0
49	32016.0
50	30805.5
51	29595.0
52	29595.0
53	29595.0
54	26497.5
55	23400.0
56	23400.0
57	23400.0
58	20714.0
59	18028.0
60	18028.0
61	18028.0
62	15410.0
63	12792.0
64	12792.0
65	12792.0
66	10203.0
67	7614.0
68	7614.0
69	7614.0
70	5662.0
71	3710.0
72	3710.0
73	3710.0
74	2623.0
75	1536.0
76	1536.0
77	1536.0
78	1081.5
79	627.0
80	627.0
81	627.0
82	460.0
83	293.0
84	293.0
85	293.0
86	202.5
87	112.0
88	112.0
89	112.0
90	70.5
91	29.0
92	29.0
93	29.0
94	21.5
95	14.0
96	14.0
97	14.0
98	49.5
99	85.0
100	85.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.879890599747812E-4
2	4.439945299873906E-4
3	8.879890599747812E-4
4	0.011543857779672154
5	0.03729554051894081
6	0.06260322872822206
7	0.11099863249684765
8	0.1127746106167972
9	0.12032251762658283
10	0.13453034258617935
11	0.1456302058358641
12	0.1354183316461541
13	0.13808229882607848
14	0.11943452856660805
15	0.1416342550659776
16	0.12387447386648195
17	0.12831441916635586
18	0.11588257232670893
19	0.10123075283712504
20	0.09501482941730158
21	0.09723480206723853
22	0.1078906707869359
23	0.08435896069760421
24	0.09235086223737723
25	0.0936828458273394
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	225228.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.943106202632414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.44754139622576	49.52236364631634
2	14.741074437918245	18.851801766494326
3	4.255222012148161	8.162763391157075
4	1.5772392273156637	4.034143016768135
5	0.7387533533905709	2.361909206670205
6	0.3570539229990744	1.369868215103781
7	0.20054333919465064	0.8976354825447859
8	0.1308391258364624	0.6693008095056403
9	0.09323921575005334	0.5365804567460236
>10	0.39587772293335616	4.559552224800745
>50	0.030612387074146503	1.366656780426409
>100	0.025742234585077742	3.2253455918266485
>500	0.004174416419201797	2.018399027602414
>1k	0.0020872082096008984	2.4236803840374592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2457	1.0908945601790185	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1568	0.6961834230202284	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1423	0.6318042161720567	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	950	0.42179480348802106	No Hit
GGTATCAACGCAGAGTACTTTTTTT	927	0.41158292929831103	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	795	0.3529756513399755	No Hit
GTACATGGGAAGCAGTGGTATCAAC	778	0.34542774433018986	No Hit
CCCATGTACTCTGCGTTGATACCAC	554	0.2459729696130144	No Hit
GTATCAACGCAGAGTACATGGGAAG	533	0.23664908448327915	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	437	0.19402560960448967	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	388	0.17226987763510754	No Hit
GTACATGGGAGTGGTATCAACGCAA	314	0.13941428241604062	No Hit
CTGTAGGACGTGGAATATGGCAAGA	311	0.13808229882607848	No Hit
TATCAACGCAGAGTACATGGGAAGC	299	0.13275436446622976	No Hit
GTATCAACGCAGAGTACATGGGAGT	294	0.13053439181629284	No Hit
GAGTACATGGGAAGCAGTGGTATCA	287	0.12742643010638108	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	286	0.1269824355763937	No Hit
GTCCTACAGTGGACATTTCTAAATT	267	0.11854653950663328	No Hit
GCGTTGATACCACTGCTTCCCATGT	258	0.11455058873674677	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	245	0.10877865984691068	No Hit
GGTATCAACGCAGAGTACATGGGAA	236	0.10478270907702417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.439945299873906E-4
2	0.0	0.0	0.0	0.0	4.439945299873906E-4
3	0.0	0.0	0.0	0.0	4.439945299873906E-4
4	0.0	0.0	0.0	0.0	4.439945299873906E-4
5	0.0	0.0	0.0	0.0	4.439945299873906E-4
6	0.0	0.0	0.0	0.0	4.439945299873906E-4
7	0.0	0.0	0.0	0.0	4.439945299873906E-4
8	0.0	0.0	0.0	0.0	4.439945299873906E-4
9	0.0	0.0	0.0	0.0	4.439945299873906E-4
10	0.0	0.0	0.0	0.0	4.439945299873906E-4
11	0.0	0.0	0.0	0.0	4.439945299873906E-4
12	0.0	0.0	0.0	0.0	0.0013319835899621717
13	0.0	0.0	0.0	0.0	0.0013319835899621717
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAATGG	25	0.006023726	18.995998	3
TACCCTG	35	0.0021622772	16.285904	5
TTGGATG	35	0.0021622772	16.285904	4
GTCCTAG	35	0.002165493	16.282284	1
GGGACGC	55	1.9482514E-4	13.818342	7
GTCCTAA	130	1.4551915E-11	13.151075	1
GACGCAG	60	4.0598592E-4	12.669633	9
GGACGCA	60	4.0674896E-4	12.666816	8
AAAGTGT	110	3.7483915E-8	12.09105	6
ACATTTC	80	2.8340988E-5	11.877781	13
ATGGCAA	105	2.6873386E-7	11.759427	17
TAGGACA	65	7.9759106E-4	11.692445	4
TGGCAAG	115	6.952905E-8	11.565353	18
GTATTTC	125	1.7900675E-8	11.402669	13
CCTAAAG	125	1.8018909E-8	11.397598	3
TCCTAAA	135	4.640242E-9	11.256887	2
TTAGGAC	110	4.9090704E-7	11.224907	3
TGGACAG	60	0.0058487044	11.083464	5
AGGACAT	60	0.0058487044	11.083464	5
CTAAAGT	130	3.2026946E-8	10.961667	4
>>END_MODULE