FastQCFastQC Report
Thu 2 Feb 2017
SRR4062329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225228
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.320564050650896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.18869767524464098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.1651659651553093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.16383398156534712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTAA250.002349033335.19911214
GATATAA300.005711064729.3586671
TAGGACC609.708972E-625.6660184
CCGTGAT1001.7262209E-924.1993927
TTCCGTG1001.7262209E-924.1993925
TCCGTGA1101.9826984E-1023.99939326
TCCTACA1700.023.2987022
TTTCCGT952.6615453E-823.1573124
GTCCTAA1205.838956E-1022.0190011
GAGTTAG500.002561106622.0190011
TCCTAGA500.002571041722.004332
TTCTCGC703.201021E-521.99944742
CGTGATT1105.1986717E-921.99944528
GTCCTAC1750.021.3898851
TTTTCCG1057.678136E-820.95185323
TTCGTCA956.817045E-720.8415844
CCTACAG1601.8189894E-1220.624483
TTTCTCG755.4483906E-520.53281641
GTACAGG654.8864994E-420.325231
AAGAGTG654.9158814E-420.307185
Produced by FastQC (version 0.11.5)