##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062329_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225228
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.899466318574955	32.0	32.0	32.0	32.0	32.0
2	31.325310352176462	32.0	32.0	32.0	32.0	32.0
3	31.513959188022802	32.0	32.0	32.0	32.0	32.0
4	31.606247891025983	32.0	32.0	32.0	32.0	32.0
5	31.61687268012858	32.0	32.0	32.0	32.0	32.0
6	35.1689843181132	36.0	36.0	36.0	36.0	36.0
7	35.1867574191486	36.0	36.0	36.0	36.0	36.0
8	35.13818885751328	36.0	36.0	36.0	36.0	36.0
9	35.22581561795159	36.0	36.0	36.0	36.0	36.0
10	35.121001829257466	36.0	36.0	36.0	36.0	36.0
11	35.23382527927256	36.0	36.0	36.0	36.0	36.0
12	35.16755021578134	36.0	36.0	36.0	36.0	36.0
13	35.163079190864366	36.0	36.0	36.0	36.0	36.0
14	35.09679080753725	36.0	36.0	36.0	36.0	36.0
15	35.0774592857016	36.0	36.0	36.0	36.0	36.0
16	35.131422380876266	36.0	36.0	36.0	36.0	36.0
17	35.111207309925945	36.0	36.0	36.0	36.0	36.0
18	35.06176407906655	36.0	36.0	36.0	36.0	36.0
19	35.073352336299216	36.0	36.0	36.0	36.0	36.0
20	35.02241728381907	36.0	36.0	36.0	36.0	36.0
21	35.02431758040741	36.0	36.0	36.0	36.0	36.0
22	34.98643152716359	36.0	36.0	36.0	36.0	36.0
23	34.94661853765962	36.0	36.0	36.0	36.0	36.0
24	34.9508009661321	36.0	36.0	36.0	36.0	36.0
25	34.89889356563127	36.0	36.0	36.0	32.0	36.0
26	34.86020388228817	36.0	36.0	36.0	32.0	36.0
27	34.83062496670041	36.0	36.0	36.0	32.0	36.0
28	34.77685722911894	36.0	36.0	36.0	32.0	36.0
29	34.693426216989	36.0	36.0	36.0	32.0	36.0
30	34.689021791251534	36.0	36.0	36.0	32.0	36.0
31	34.63154225939937	36.0	36.0	36.0	32.0	36.0
32	34.58175715275188	36.0	36.0	36.0	32.0	36.0
33	34.48695988065427	36.0	36.0	36.0	32.0	36.0
34	34.43239739286412	36.0	36.0	36.0	32.0	36.0
35	34.37571261122063	36.0	36.0	36.0	32.0	36.0
36	34.28323743051486	36.0	36.0	36.0	32.0	36.0
37	34.24196813895253	36.0	36.0	36.0	32.0	36.0
38	34.21648729287655	36.0	36.0	36.0	32.0	36.0
39	34.124025432006675	36.0	36.0	36.0	32.0	36.0
40	34.09796739304172	36.0	36.0	36.0	32.0	36.0
41	34.037788374447224	36.0	36.0	36.0	32.0	36.0
42	33.9894062905145	36.0	36.0	36.0	32.0	36.0
43	33.92689630063757	36.0	36.0	36.0	32.0	36.0
44	33.873275081251	36.0	36.0	36.0	32.0	36.0
45	33.75074147086508	36.0	36.0	36.0	32.0	36.0
46	33.65692542667875	36.0	36.0	36.0	27.0	36.0
47	33.58362192977783	36.0	36.0	36.0	21.0	36.0
48	33.52031274974692	36.0	36.0	36.0	21.0	36.0
49	33.475202905500204	36.0	36.0	36.0	21.0	36.0
50	33.026275596284655	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	6.0
20	20.0
21	22.0
22	55.0
23	126.0
24	296.0
25	630.0
26	1215.0
27	2163.0
28	3551.0
29	5024.0
30	6532.0
31	9026.0
32	12366.0
33	18691.0
34	36926.0
35	128578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35186583405136	18.59127530490242	11.778160725547847	25.278698135498367
2	14.546923842000567	21.294668229610686	38.93062659846547	25.22778132992327
3	18.82670005505532	26.38837089527057	28.494680945530753	26.290248104143355
4	11.81513843749445	16.598291509048607	36.22817766885112	35.35839238460582
5	12.676487825669987	38.4716820288774	33.93361393787628	14.918216207576323
6	32.735563389661934	35.88528855460737	17.476223881789846	13.902924173940843
7	27.474825510149714	31.805104161116738	22.07318805832312	18.64688227041043
8	27.407782336121617	33.80574351323992	20.030813220381123	18.75566093025734
9	27.645763404194863	13.973395847763156	19.311098087271564	39.069742660770416
10	14.785905837640081	28.425861793382705	33.53357486635765	23.25465750261957
11	34.94192551547765	21.947537606336688	23.51972223702204	19.59081464116362
12	24.587085087111728	24.228781501411902	31.021453815688993	20.16267959578738
13	30.070861526985986	21.14834745235939	25.516809632905325	23.263981387749304
14	22.45102740334239	20.251922496314844	28.171008933169944	29.126041167172822
15	24.046299749587085	28.109293693501698	24.904097181522726	22.940309375388495
16	22.579442073996457	26.562534687226663	27.44386774232219	23.41415549645469
17	21.94265366650683	26.495373576997533	28.20919246274886	23.35278029374678
18	22.346787907311292	24.879787947270977	30.746313719047897	22.027110426369838
19	23.62539293515904	25.001331983589964	29.64462677819809	21.728648303052907
20	24.338892144848774	24.68343190011899	29.53451613476122	21.443159820271017
21	23.842062265792883	24.484966345214627	28.73843394249383	22.93453744649866
22	23.784342976894525	25.772550482178058	28.050686415543357	22.392420125384056
23	21.78059566306143	25.988775818281916	29.423073507734387	22.807555010922265
24	22.789413159402013	25.47164365983936	29.354462022084775	22.384481158673847
25	22.526062478910262	24.95604454153125	29.588239472889693	22.9296535066688
26	21.90875790875791	25.696525696525697	29.64013764013764	22.754578754578755
27	22.399524037863852	26.0247393752109	29.11405331486316	22.46168327206209
28	22.14240045465847	25.90532092494583	29.099918303555572	22.852360316840123
29	22.856698856698856	25.300921300921303	29.35819735819736	22.484182484182487
30	22.233766233766232	25.7982017982018	29.860361860361863	22.107670107670106
31	23.377643987426076	24.916529028362373	29.261459498818976	22.44436748539258
32	21.443889443889443	26.56721056721057	29.45765345765346	22.53124653124653
33	21.622377622377623	26.373626373626376	30.007770007770006	21.996225996225995
34	22.038947891876532	26.07803786452598	29.683337477355877	22.19967676624161
35	21.93776972667697	26.452306107588754	29.865736054131816	21.744188111602465
36	22.243336722506626	26.13185808095832	29.840116859879146	21.784688336655908
37	21.997948681517265	26.484652852087503	28.866757540371456	22.650640926023772
38	22.436375583852808	27.126289804109614	28.559948141438895	21.87738647059868
39	22.77958335553306	26.610368160264265	28.45738540501181	22.152663079190866
40	22.807555010922265	26.549540909655995	28.937787486458166	21.705116592963574
41	22.47273825169609	26.671225091464496	28.9258693567293	21.930167300110114
42	22.455331770389314	27.228083262290426	28.810120062517765	21.5064649048025
43	22.161588533572512	27.166161961512163	28.54041045405703	22.131839050858297
44	21.775289280417738	27.405045867486034	28.39965543882707	22.420009413269156
45	22.339688657212122	27.301547833654503	28.07901677485814	22.279746734275236
46	22.099724713613355	27.33860225557233	27.71778705265962	22.84388597815469
47	22.712943558412956	27.570773985010476	27.68266259368451	22.033619862892053
48	21.968936763491374	28.121642142279924	27.228285483398306	22.681135610830395
49	22.623757320652345	27.823585043890613	27.024362953392036	22.528294682065013
50	21.95049395049395	29.0989010989011	26.701298701298704	22.24930624930625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	424.0
1	290.0
2	156.0
3	403.0
4	650.0
5	643.5
6	637.0
7	535.0
8	433.0
9	508.5
10	584.0
11	790.5
12	997.0
13	1558.0
14	2119.0
15	2497.0
16	2875.0
17	2679.0
18	2483.0
19	2247.5
20	2012.0
21	2089.0
22	2166.0
23	2200.5
24	2235.0
25	2382.5
26	2530.0
27	2715.0
28	2900.0
29	3565.0
30	4230.0
31	4926.0
32	5622.0
33	6760.0
34	7898.0
35	8821.5
36	9745.0
37	9899.5
38	10054.0
39	10322.0
40	10590.0
41	10969.0
42	11348.0
43	11686.0
44	12024.0
45	12879.0
46	13734.0
47	14417.0
48	15100.0
49	15130.5
50	15161.0
51	14888.5
52	14616.0
53	14495.5
54	14375.0
55	13841.5
56	13308.0
57	12622.5
58	11937.0
59	11009.0
60	10081.0
61	8686.5
62	7292.0
63	6265.5
64	5239.0
65	4409.5
66	3580.0
67	2936.0
68	2292.0
69	1850.0
70	1408.0
71	1141.5
72	875.0
73	725.5
74	576.0
75	491.0
76	406.0
77	309.0
78	212.0
79	183.5
80	155.0
81	123.5
82	92.0
83	68.5
84	45.0
85	27.5
86	10.0
87	10.5
88	11.0
89	8.0
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0328555952190669
2	0.005327934359848687
3	0.0
4	0.0
5	0.0
6	8.879890599747812E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.439945299873906E-4
17	0.0
18	4.439945299873906E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.002219972649936953
25	0.0
26	0.0013319835899621717
27	0.0
28	0.0017759781199495623
29	0.0013319835899621717
30	0.0013319835899621717
31	0.0
32	0.0013319835899621717
33	0.0013319835899621717
34	0.0017759781199495623
35	0.0
36	4.439945299873906E-4
37	0.0031079617099117342
38	0.0
39	0.0
40	0.0
41	0.0017759781199495623
42	0.005327934359848687
43	0.0062159234198234685
44	0.0062159234198234685
45	0.004439945299873906
46	0.0035519562398991246
47	0.0017759781199495623
48	0.004439945299873906
49	0.003995950769886515
50	0.0013319835899621717
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	225228.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.95337299194819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57504249907828	54.965892557962036
2	14.10719514189427	19.73691767262259
3	4.084846929495626	8.572464626220654
4	1.4897305154792073	4.16846697627217
5	0.6680424010205535	2.336590962651371
6	0.34893856544472757	1.464565777189821
7	0.1837637690964041	0.8998426837404899
8	0.11532296128347708	0.6453784100159258
9	0.08214083717438755	0.5171425758657734
>10	0.30997106695348026	3.8156911961550084
>50	0.022912568561204823	1.0992615227267042
>100	0.011456284280602412	1.4563322968161398
>500	6.36460237811245E-4	0.32145274176131244
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	722	0.320564050650896	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.18869767524464098	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.1651659651553093	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	369	0.16383398156534712	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.439945299873906E-4
2	0.0	0.0	0.0	0.0	4.439945299873906E-4
3	0.0	0.0	0.0	0.0	4.439945299873906E-4
4	0.0	0.0	0.0	0.0	4.439945299873906E-4
5	0.0	0.0	0.0	0.0	4.439945299873906E-4
6	0.0	0.0	0.0	0.0	4.439945299873906E-4
7	0.0	0.0	0.0	0.0	4.439945299873906E-4
8	0.0	0.0	0.0	0.0	4.439945299873906E-4
9	0.0	0.0	0.0	0.0	4.439945299873906E-4
10	0.0	0.0	0.0	0.0	4.439945299873906E-4
11	0.0	0.0	0.0	0.0	4.439945299873906E-4
12	0.0	0.0	0.0	0.0	8.879890599747812E-4
13	0.0	0.0	0.0	0.0	8.879890599747812E-4
14	0.0	0.0	0.0	0.0	8.879890599747812E-4
15	0.0	0.0	0.0	0.0	8.879890599747812E-4
16	0.0	0.0	0.0	0.0	8.879890599747812E-4
17	0.0	0.0	0.0	4.439945299873906E-4	8.879890599747812E-4
18	0.0	0.0	0.0	0.0013319835899621717	8.879890599747812E-4
19	0.0	0.0	0.0	0.0013319835899621717	8.879890599747812E-4
20	0.0	0.0	0.0	0.003107961709911734	8.879890599747812E-4
21	0.0	0.0	0.0	0.0035519562398991246	8.879890599747812E-4
22	0.0	0.0	0.0	0.006659917949810858	0.0013319835899621717
23	0.0	0.0	0.0	0.00799190153977303	0.0013319835899621717
24	0.0	0.0	0.0	0.012431846839646935	0.0013319835899621717
25	0.0	0.0	0.0	0.014207824959596499	0.0013319835899621717
26	0.0	0.0	0.0	0.01553980854955867	0.0013319835899621717
27	0.0	0.0	0.0	0.019535759319445185	0.0013319835899621717
28	0.0	0.0	0.0	0.02397570461931909	0.0013319835899621717
29	0.0	0.0	0.0	0.03596355692897864	0.0013319835899621717
30	0.0	0.0	0.0	0.04883939829861296	0.0013319835899621717
31	0.0	0.0	0.0	0.07725504821780596	0.0013319835899621717
32	0.0	0.0	0.0	0.10078675830713765	0.0013319835899621717
33	0.0	0.0	0.0	0.12875841369634325	0.0013319835899621717
34	0.0	0.0	0.0	0.1549540909655993	0.0013319835899621717
35	0.0	0.0	0.0	0.19890954943435096	0.0013319835899621717
36	0.0	0.0	0.0	0.23398511730335483	0.0013319835899621717
37	0.0	0.0	0.0	0.29481236791162735	0.0013319835899621717
38	0.0	0.0	0.0	0.37295540518940806	0.0013319835899621717
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACTAA	25	0.0023490333	35.199112	14
GATATAA	30	0.0057110647	29.358667	1
TAGGACC	60	9.708972E-6	25.666018	4
CCGTGAT	100	1.7262209E-9	24.19939	27
TTCCGTG	100	1.7262209E-9	24.19939	25
TCCGTGA	110	1.9826984E-10	23.999393	26
TCCTACA	170	0.0	23.298702	2
TTTCCGT	95	2.6615453E-8	23.15731	24
GTCCTAA	120	5.838956E-10	22.019001	1
GAGTTAG	50	0.0025611066	22.019001	1
TCCTAGA	50	0.0025710417	22.00433	2
TTCTCGC	70	3.201021E-5	21.999447	42
CGTGATT	110	5.1986717E-9	21.999445	28
GTCCTAC	175	0.0	21.389885	1
TTTTCCG	105	7.678136E-8	20.951853	23
TTCGTCA	95	6.817045E-7	20.84158	44
CCTACAG	160	1.8189894E-12	20.62448	3
TTTCTCG	75	5.4483906E-5	20.532816	41
GTACAGG	65	4.8864994E-4	20.32523	1
AAGAGTG	65	4.9158814E-4	20.30718	5
>>END_MODULE