FastQCFastQC Report
Thu 2 Feb 2017
SRR4062328_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062328_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517393
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC23220.1530256169627776No Hit
GTATCAACGCAGAGTACTTTTTTTT18930.12475344225260035No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16100.10610303329460463No Hit
TCGTAGTTCCGACCATAAACGATGC15570.10261020052155242No Hit
GGGTAGGCACACGCTGAGCCAGTCA15490.102082980480337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.014.1123541
TACACCG500.001499458413.3000695
GCGTTAT1450.013.09703351
TCGCGTA1457.2759576E-1212.4487519
CGACCAT2600.012.42478210
CGAACGA853.937086E-612.29580416
CGGTCCA2750.012.09256710
CGCCGGT2600.012.0585527
TAGGACA1501.4551915E-1112.0322054
GTATCAA14300.011.95218851
CGCGTAA1603.6379788E-1211.87662910
CGTTATT1603.6379788E-1211.8719282
GGTCCAA3150.011.76390711
ACTGCGT658.0184283E-411.6931418
CGAGCCG3350.011.62846115
AAGACGG4100.011.5854265
CAAGACG4300.011.4872944
AACACCG752.0736497E-411.400065
GTTATTC2000.011.3981793
CCGGTCC3100.011.3391429
Produced by FastQC (version 0.11.5)