##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062328_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1517393
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26869703498039	32.0	32.0	32.0	32.0	32.0
2	30.842458084359162	32.0	32.0	32.0	32.0	32.0
3	30.862556371355343	32.0	32.0	32.0	32.0	32.0
4	30.866677914027544	32.0	32.0	32.0	32.0	32.0
5	30.805749070939434	32.0	32.0	32.0	32.0	32.0
6	34.44131810282504	36.0	36.0	36.0	32.0	36.0
7	34.37531213074003	36.0	36.0	36.0	32.0	36.0
8	34.33127541777245	36.0	36.0	36.0	32.0	36.0
9	34.465949164125576	36.0	36.0	36.0	32.0	36.0
10	34.183017188032366	36.0	36.0	36.0	32.0	36.0
11	34.43732441101284	36.0	36.0	36.0	32.0	36.0
12	34.27949120629923	36.0	36.0	36.0	32.0	36.0
13	34.35450143766315	36.0	36.0	36.0	32.0	36.0
14	34.25834045629576	36.0	36.0	36.0	32.0	36.0
15	34.2192503853649	36.0	36.0	36.0	32.0	36.0
16	34.22175797568593	36.0	36.0	36.0	32.0	36.0
17	34.1586978455812	36.0	36.0	36.0	32.0	36.0
18	34.162314575063945	36.0	36.0	36.0	32.0	36.0
19	34.16917502585026	36.0	36.0	36.0	32.0	36.0
20	34.146030725065955	36.0	36.0	36.0	32.0	36.0
21	34.134112916034276	36.0	36.0	36.0	32.0	36.0
22	34.103006933602565	36.0	36.0	36.0	32.0	36.0
23	34.07258567819939	36.0	36.0	36.0	32.0	36.0
24	34.04472737122156	36.0	36.0	36.0	32.0	36.0
25	33.69775727184717	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	23.0
5	109.0
6	304.0
7	73.0
8	268.0
9	219.0
10	104.0
11	25.0
12	56.0
13	64.0
14	163.0
15	265.0
16	406.0
17	580.0
18	733.0
19	1153.0
20	1684.0
21	2482.0
22	3782.0
23	5786.0
24	8089.0
25	11583.0
26	16837.0
27	21785.0
28	29192.0
29	39281.0
30	52407.0
31	72341.0
32	102540.0
33	145442.0
34	317513.0
35	682103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.29465766776879	17.190258677673423	11.48558921977552	26.029494434782265
2	17.011315482506145	18.844858605895652	37.22549904770692	26.918326863891284
3	20.129881256318434	21.56432180078108	27.531689365790907	30.774107577109582
4	13.203312840089426	14.939943898426625	34.58755589066521	37.26918737081875
5	15.50591959581017	35.531550631513646	33.155076336053995	15.807453436622188
6	35.99236633670549	34.14112385513185	15.860293938240922	14.006215869921746
7	31.634016248791895	29.442309531898893	19.30954179462398	19.61413242468523
8	28.316937633886486	33.30505237365437	18.491855149586705	19.88615484287244
9	26.340667468545604	15.255537880202452	18.588855818163943	39.814938833088
10	16.743060238960325	26.33671154376606	30.39254374098422	26.527684476289398
11	37.728556023531056	21.238139108834442	21.1953085712551	19.8379962963794
12	24.81072266886811	23.392784199755965	27.89221635126494	23.904276780110983
13	29.374210215481263	19.168956973034323	24.750947741422486	26.70588507006193
14	23.275802347116446	19.603641217249134	24.513623554825102	32.60693288080932
15	25.235849679154686	27.13247968155674	21.622017564004086	26.009653075284483
16	26.048999036647004	26.014159969383847	23.092627050424273	24.844213943544872
17	24.31772232750384	26.35175439059539	24.65740950912959	24.67311377277118
18	25.03165235520573	25.277415821889115	25.521661822872165	24.169270000032988
19	25.53687790834979	25.163566845702935	24.820007203669913	24.47954804227736
20	25.64779621975401	24.630768358019136	24.58142753297194	25.14000788925491
21	26.360376619213277	24.415011998569934	24.50702961845999	24.717581763756797
22	25.699097600219545	24.438944035908637	25.00192961426846	24.86002874960336
23	24.490884867032502	24.692644575770366	25.344356796116507	25.47211376108062
24	24.67747148727894	25.0648091346249	25.116128734358405	25.141590643737754
25	24.79438329499904	24.7728800322417	24.981910215303067	25.450826457456188
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	141.0
1	141.0
2	256.5
3	372.0
4	372.0
5	372.0
6	842.0
7	1312.0
8	1312.0
9	1312.0
10	1649.0
11	1986.0
12	1986.0
13	1986.0
14	2850.5
15	3715.0
16	3715.0
17	3715.0
18	6392.5
19	9070.0
20	9070.0
21	9070.0
22	14343.0
23	19616.0
24	19616.0
25	19616.0
26	28946.5
27	38277.0
28	38277.0
29	38277.0
30	48418.5
31	58560.0
32	58560.0
33	58560.0
34	74338.0
35	90116.0
36	90116.0
37	90116.0
38	104877.0
39	119638.0
40	119638.0
41	119638.0
42	136966.0
43	154294.0
44	154294.0
45	154294.0
46	172312.5
47	190331.0
48	190331.0
49	190331.0
50	197514.0
51	204697.0
52	204697.0
53	204697.0
54	194335.0
55	183973.0
56	183973.0
57	183973.0
58	171352.5
59	158732.0
60	158732.0
61	158732.0
62	140493.5
63	122255.0
64	122255.0
65	122255.0
66	99899.0
67	77543.0
68	77543.0
69	77543.0
70	59036.5
71	40530.0
72	40530.0
73	40530.0
74	30608.0
75	20686.0
76	20686.0
77	20686.0
78	16778.0
79	12870.0
80	12870.0
81	12870.0
82	8870.0
83	4870.0
84	4870.0
85	4870.0
86	3581.0
87	2292.0
88	2292.0
89	2292.0
90	1500.0
91	708.0
92	708.0
93	708.0
94	445.0
95	182.0
96	182.0
97	182.0
98	404.5
99	627.0
100	627.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014498551133424234
2	1.9770751545578503E-4
3	4.613175360634984E-4
4	0.009555863247029608
5	0.03176500748322946
6	0.06273918490463577
7	0.10465317818126221
8	0.10873913350068178
9	0.11770187420134402
10	0.13147549777809703
11	0.13964740841693615
12	0.13338667042750296
13	0.1283780800359564
14	0.11506577399526688
15	0.13318896291204718
16	0.12211734204652322
17	0.1239626121907772
18	0.1125614787994936
19	0.0987878552227406
20	0.09272482474876317
21	0.09087955460450918
22	0.1018852729648812
23	0.08125778885232764
24	0.09246121472815545
25	0.08857296692419168
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1517393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.7036847952179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.90104461685773	33.55734759080684
2	16.388606676711863	15.963710676307564
3	6.499323102456218	9.496229512929162
4	3.0609222526679862	5.963127703064397
5	1.6332345017464716	3.9772269184867453
6	0.95709505998871	2.7968433672450175
7	0.6031047867231019	2.0561397801734347
8	0.39016463344226954	1.520196426033122
9	0.28052552999163727	1.2296364290751731
>10	1.132177031451785	9.374786478454679
>50	0.07147532518830017	2.4183732600465757
>100	0.07120509387069274	7.04378780483829
>500	0.008544481718422115	2.794399075728497
>1k	0.002576907184920955	1.8081949768105114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	2322	0.1530256169627776	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1893	0.12475344225260035	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1610	0.10610303329460463	No Hit
TCGTAGTTCCGACCATAAACGATGC	1557	0.10261020052155242	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1549	0.102082980480337	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.613175360634984E-4
2	0.0	6.590250515192834E-5	0.0	0.0	4.613175360634984E-4
3	0.0	6.590250515192834E-5	0.0	0.0	4.613175360634984E-4
4	0.0	1.3180501030385667E-4	0.0	0.0	4.613175360634984E-4
5	0.0	1.3180501030385667E-4	0.0	0.0	5.272200412154267E-4
6	0.0	1.3180501030385667E-4	0.0	0.0	5.931225463673551E-4
7	0.0	1.3180501030385667E-4	0.0	0.0	5.931225463673551E-4
8	0.0	1.3180501030385667E-4	0.0	0.0	5.931225463673551E-4
9	0.0	1.3180501030385667E-4	0.0	0.0	5.931225463673551E-4
10	0.0	1.3180501030385667E-4	0.0	0.0	6.590250515192835E-4
11	0.0	1.3180501030385667E-4	0.0	0.0	7.249275566712117E-4
12	0.0	1.3180501030385667E-4	0.0	0.0	0.0023724901854694203
13	0.0	1.3180501030385667E-4	0.0	0.0	0.002636100206077134
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	545	0.0	14.112354	1
TACACCG	50	0.0014994584	13.300069	5
GCGTTAT	145	0.0	13.0970335	1
TCGCGTA	145	7.2759576E-12	12.448751	9
CGACCAT	260	0.0	12.424782	10
CGAACGA	85	3.937086E-6	12.295804	16
CGGTCCA	275	0.0	12.092567	10
CGCCGGT	260	0.0	12.058552	7
TAGGACA	150	1.4551915E-11	12.032205	4
GTATCAA	1430	0.0	11.9521885	1
CGCGTAA	160	3.6379788E-12	11.876629	10
CGTTATT	160	3.6379788E-12	11.871928	2
GGTCCAA	315	0.0	11.763907	11
ACTGCGT	65	8.0184283E-4	11.693141	8
CGAGCCG	335	0.0	11.628461	15
AAGACGG	410	0.0	11.585426	5
CAAGACG	430	0.0	11.487294	4
AACACCG	75	2.0736497E-4	11.40006	5
GTTATTC	200	0.0	11.398179	3
CCGGTCC	310	0.0	11.339142	9
>>END_MODULE