FastQCFastQC Report
Thu 2 Feb 2017
SRR4062328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517393
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT24410.16086801507585707No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT17220.11348411387162059No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15940.10504859321217377No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG15630.103005615552464No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15440.10175346795457736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14750.025.8139271
GGTATCA7150.020.6238021
TCCGTAT708.123934E-418.85738412
GTACTAG1303.5723133E-818.6229861
ATACCGT4450.017.7973926
TTGACGG3600.017.72186333
TACCGTC4550.017.406247
ATCAACG21700.017.3360563
ACCGTCG4600.017.2170438
TACGAAT3850.017.14307441
CGCAATA3750.017.01298936
TCAACGC22000.016.9996574
AACGCAG22050.016.9611076
CAACGCA22550.016.5850325
CGAGCCG4650.016.5593515
CGTCGTA4650.016.5593510
TAAGGTG2151.2732926E-1116.3717615
TATCAAC23750.016.3033662
ATTGACG4100.016.09723532
CCGTCGT4950.015.9996769
Produced by FastQC (version 0.11.5)