FastQCFastQC Report
Thu 2 Feb 2017
SRR4062327_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062327_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767594
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30360.17175889938526606No Hit
GCGCAAGACGGACCAGAGCGAAAGC22420.1268390818253513No Hit
TATCAACGCAGAGTACTTTTTTTTT18820.10647241391405493No Hit
GGGTAGGCACACGCTGAGCCAGTCA18230.10313454333970358No Hit
GAATAGGACCGCGGTTCTATTTTGT17970.10166361732388772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.014.3269851
CGCATCG2650.013.6238213
CGTAAAT500.001500952213.2985133
GTACTAG500.001503196213.29587651
GTCCTAT2550.012.2903051
TCACGAG550.003071300212.0895583
CGGTCCA3000.012.03471510
GCATCGC3000.012.03403414
GATAATC1353.7107384E-1011.9636587
CGGTCGG1459.640644E-1111.79445710
TCTAGAT2500.011.7783492
CCGACCA3150.011.7625899
ATCGCCA3150.011.76225616
GTATCAA21250.011.7540031
CTATTCC2450.011.632344
CGCCAGT3300.011.51419118
CGACCAT3550.011.50836410
AGCACCG1251.830449E-811.4006635
TCGCGTA2050.011.1225999
GTGTAGG2050.011.1185041
Produced by FastQC (version 0.11.5)