##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062327_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1767594
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168901342729157	32.0	32.0	32.0	32.0	32.0
2	30.68205877594063	32.0	32.0	32.0	32.0	32.0
3	30.709662965590514	32.0	32.0	32.0	32.0	32.0
4	30.714685046452974	32.0	32.0	32.0	32.0	32.0
5	30.628655675454883	32.0	32.0	32.0	32.0	32.0
6	34.268408921958326	36.0	36.0	36.0	32.0	36.0
7	34.20179407714667	36.0	36.0	36.0	32.0	36.0
8	34.155690164144026	36.0	36.0	36.0	32.0	36.0
9	34.29411505130703	36.0	36.0	36.0	32.0	36.0
10	33.972377706645304	36.0	36.0	36.0	32.0	36.0
11	34.2735407565312	36.0	36.0	36.0	32.0	36.0
12	34.0851445524255	36.0	36.0	36.0	32.0	36.0
13	34.1845712307238	36.0	36.0	36.0	32.0	36.0
14	34.0858183496889	36.0	36.0	36.0	32.0	36.0
15	34.028165404499	36.0	36.0	36.0	32.0	36.0
16	34.032254578822965	36.0	36.0	36.0	32.0	36.0
17	33.96017015219558	36.0	36.0	36.0	32.0	36.0
18	33.97347128356399	36.0	36.0	36.0	32.0	36.0
19	33.972540639988594	36.0	36.0	36.0	32.0	36.0
20	33.96233637362426	36.0	36.0	36.0	32.0	36.0
21	33.94242965296329	36.0	36.0	36.0	32.0	36.0
22	33.91330871229479	36.0	36.0	36.0	32.0	36.0
23	33.88302970025922	36.0	36.0	36.0	32.0	36.0
24	33.85832380060127	36.0	36.0	36.0	32.0	36.0
25	33.50473072436317	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	28.0
5	154.0
6	418.0
7	94.0
8	316.0
9	236.0
10	110.0
11	32.0
12	72.0
13	73.0
14	381.0
15	520.0
16	742.0
17	1048.0
18	1402.0
19	1965.0
20	3045.0
21	4305.0
22	6486.0
23	9474.0
24	13102.0
25	17672.0
26	23404.0
27	29772.0
28	37992.0
29	49411.0
30	63514.0
31	83583.0
32	117588.0
33	164248.0
34	355441.0
35	780962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.040549202554224	16.83922830127728	11.218212913580764	25.902009582587727
2	17.365196626146403	19.031530448468622	36.52039807806435	27.08287484732062
3	19.90187669865782	22.19202386097992	27.50937444132403	30.396724999038227
4	13.371491035995206	15.050396004983584	34.00680773247965	37.571305226541554
5	15.554906594948973	35.75640886329268	32.659961243468885	16.02872329828946
6	36.07792651872585	33.77802762935764	15.91507386284507	14.228971989071445
7	31.683974139322558	29.460952699201126	19.34878215239449	19.50629100908182
8	28.900125677591387	32.537639560745774	18.356970801906854	20.205263959755985
9	26.88357929628954	14.622343866295239	18.231921524468095	40.26215531294712
10	17.03909538458052	26.06088931944232	29.864375669199	27.035639626778156
11	37.73174207036041	21.23208625226976	21.152088745109925	19.8840829322599
12	24.96697228164316	23.223358018200933	27.570950110583368	24.238719589572533
13	29.51321520645202	18.997585081548106	24.743992019382794	26.74520769261708
14	23.964804719892335	19.08506772157348	24.135468069583972	32.81465948895021
15	25.63000454353351	26.535368745701486	21.408767320097034	26.425859390667966
16	26.43861572056112	25.48324879134698	22.631195851353382	25.44693963673852
17	24.570285260457293	25.830411731950225	24.206041016281095	25.393261991311388
18	25.430151194364107	24.768654611445097	24.92962501033677	24.87156918385402
19	25.934961835865337	24.745921664701545	24.615725277127456	24.703391222305658
20	26.138525162480615	24.090904200287333	24.082409962495106	25.68816067473695
21	26.907896152552414	23.897246613337803	24.026189508114285	25.168667725995498
22	26.55978505877582	23.821368619215583	24.345469085208517	25.273377236800076
23	25.029783812554502	23.984915574782285	24.947000668153972	26.038299944509248
24	25.13672899175605	24.466250109009447	24.72775952459423	25.669261374640268
25	25.35470079420134	24.064215637696957	24.559253648937528	26.02182991916418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	493.0
1	493.0
2	583.0
3	673.0
4	673.0
5	673.0
6	1282.0
7	1891.0
8	1891.0
9	1891.0
10	2214.5
11	2538.0
12	2538.0
13	2538.0
14	3141.5
15	3745.0
16	3745.0
17	3745.0
18	6223.0
19	8701.0
20	8701.0
21	8701.0
22	13597.5
23	18494.0
24	18494.0
25	18494.0
26	27761.5
27	37029.0
28	37029.0
29	37029.0
30	47657.5
31	58286.0
32	58286.0
33	58286.0
34	75385.0
35	92484.0
36	92484.0
37	92484.0
38	111995.0
39	131506.0
40	131506.0
41	131506.0
42	154156.0
43	176806.0
44	176806.0
45	176806.0
46	199630.0
47	222454.0
48	222454.0
49	222454.0
50	234746.0
51	247038.0
52	247038.0
53	247038.0
54	236036.0
55	225034.0
56	225034.0
57	225034.0
58	210356.5
59	195679.0
60	195679.0
61	195679.0
62	172832.0
63	149985.0
64	149985.0
65	149985.0
66	122815.5
67	95646.0
68	95646.0
69	95646.0
70	73202.0
71	50758.0
72	50758.0
73	50758.0
74	37825.5
75	24893.0
76	24893.0
77	24893.0
78	19512.0
79	14131.0
80	14131.0
81	14131.0
82	9669.5
83	5208.0
84	5208.0
85	5208.0
86	3827.5
87	2447.0
88	2447.0
89	2447.0
90	1603.5
91	760.0
92	760.0
93	760.0
94	477.5
95	195.0
96	195.0
97	195.0
98	457.5
99	720.0
100	720.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.354630079079245E-4
2	5.6574077531378816E-5
3	0.0012446297056903338
4	0.01086222288602473
5	0.03767833563589829
6	0.06856778196803112
7	0.11196009943459867
8	0.11116806234915938
9	0.12174741484752721
10	0.13713556393606224
11	0.1436415828521708
12	0.13634352685062293
13	0.1310821376402047
14	0.12050278514183688
15	0.13849334179681533
16	0.12440639649150202
17	0.12983750793451437
18	0.11609000709438932
19	0.10183333955648187
20	0.09566676510556157
21	0.09594963549321847
22	0.1076038954646825
23	0.0867846349331351
24	0.09572333918309295
25	0.09442213539987124
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1767594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.17590030589521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.77251092700614	34.57705331123223
2	15.368880195693842	14.808192802419876
3	5.476793032769195	7.915483054281307
4	2.4631098968396716	4.746501473304475
5	1.3325106311228185	3.209744966075921
6	0.7866681054149225	2.2739066532178063
7	0.5355469406389213	1.8060319214943499
8	0.3791037478601491	1.4610931490001422
9	0.2778809253127618	1.2048447379299774
>10	1.4211559234386482	12.249606100252104
>50	0.10208432322184793	3.3893604368057315
>100	0.07172336884048723	7.129156058248425
>500	0.008729084864796236	2.8559204930498896
>1k	0.0033028969758688463	2.3731048426876997
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3036	0.17175889938526606	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2242	0.1268390818253513	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1882	0.10647241391405493	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1823	0.10313454333970358	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1797	0.10166361732388772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1314815506275762E-4
2	0.0	0.0	0.0	0.0	1.1314815506275762E-4
3	0.0	0.0	0.0	0.0	2.2629631012551524E-4
4	0.0	0.0	0.0	0.0	2.2629631012551524E-4
5	0.0	0.0	0.0	0.0	3.394444651882729E-4
6	0.0	0.0	0.0	0.0	3.394444651882729E-4
7	0.0	0.0	0.0	5.657407753137881E-5	3.394444651882729E-4
8	0.0	0.0	0.0	5.657407753137881E-5	3.394444651882729E-4
9	0.0	0.0	0.0	5.657407753137881E-5	3.394444651882729E-4
10	0.0	0.0	0.0	5.657407753137881E-5	3.394444651882729E-4
11	0.0	0.0	0.0	5.657407753137881E-5	3.960185427196517E-4
12	0.0	0.0	0.0	5.657407753137881E-5	7.354630079079245E-4
13	0.0	0.0	0.0	5.657407753137881E-5	9.05185240502061E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	875	0.0	14.326985	1
CGCATCG	265	0.0	13.62382	13
CGTAAAT	50	0.0015009522	13.298513	3
GTACTAG	50	0.0015031962	13.2958765	1
GTCCTAT	255	0.0	12.290305	1
TCACGAG	55	0.0030713002	12.089558	3
CGGTCCA	300	0.0	12.034715	10
GCATCGC	300	0.0	12.034034	14
GATAATC	135	3.7107384E-10	11.963658	7
CGGTCGG	145	9.640644E-11	11.794457	10
TCTAGAT	250	0.0	11.778349	2
CCGACCA	315	0.0	11.762589	9
ATCGCCA	315	0.0	11.762256	16
GTATCAA	2125	0.0	11.754003	1
CTATTCC	245	0.0	11.63234	4
CGCCAGT	330	0.0	11.514191	18
CGACCAT	355	0.0	11.508364	10
AGCACCG	125	1.830449E-8	11.400663	5
TCGCGTA	205	0.0	11.122599	9
GTGTAGG	205	0.0	11.118504	1
>>END_MODULE