FastQCFastQC Report
Thu 2 Feb 2017
SRR4062327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767594
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22620.12797056337597887No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19650.11116806234915938No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18440.10432259896786253No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18320.10364371003748597No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18290.10347398780489185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22450.026.8564761
GGTATCA9550.023.9631981
AGTATCG852.3542634E-723.2937878
TTAGTAC654.933875E-420.3074043
GAGCGAA8650.019.07487716
TCAACGC31900.018.8962844
CGGATCG951.5979851E-518.52605426
AGCGAAA8800.018.49973717
AACGCAG32500.018.4797386
GACGGAC8850.018.395227
CAACGCA32850.018.2828465
ATCAACG32850.018.2828463
GCGAAAG8900.018.0446918
CGCAATA4050.017.9261836
AGAGCGA9250.017.83758515
TATCAAC34750.017.4098262
ACGGACC9600.017.1872588
AAGACGG10050.017.0743855
TACACCG1551.4406396E-817.0320175
CAAGACG10550.016.8907554
Produced by FastQC (version 0.11.5)