##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062327_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1767594
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26760160987195	32.0	32.0	32.0	32.0	32.0
2	31.428098307643044	32.0	32.0	32.0	32.0	32.0
3	31.550803521623177	32.0	32.0	32.0	32.0	32.0
4	31.636037461091178	32.0	32.0	32.0	32.0	32.0
5	31.607606158427785	32.0	32.0	32.0	32.0	32.0
6	35.23493404028301	36.0	36.0	36.0	36.0	36.0
7	35.241816276814696	36.0	36.0	36.0	36.0	36.0
8	35.185430025220725	36.0	36.0	36.0	36.0	36.0
9	35.26639658202053	36.0	36.0	36.0	36.0	36.0
10	35.15002653324236	36.0	36.0	36.0	36.0	36.0
11	35.2751265279244	36.0	36.0	36.0	36.0	36.0
12	35.19298266457116	36.0	36.0	36.0	36.0	36.0
13	35.2319435345447	36.0	36.0	36.0	36.0	36.0
14	35.18719909662513	36.0	36.0	36.0	36.0	36.0
15	35.16815003897954	36.0	36.0	36.0	36.0	36.0
16	35.17659258856955	36.0	36.0	36.0	36.0	36.0
17	35.15693139940507	36.0	36.0	36.0	36.0	36.0
18	35.15632322807161	36.0	36.0	36.0	36.0	36.0
19	35.14824048961469	36.0	36.0	36.0	36.0	36.0
20	35.14784107662732	36.0	36.0	36.0	36.0	36.0
21	35.140264110423544	36.0	36.0	36.0	36.0	36.0
22	35.13370774057844	36.0	36.0	36.0	36.0	36.0
23	35.10031262835244	36.0	36.0	36.0	36.0	36.0
24	35.081798195739516	36.0	36.0	36.0	36.0	36.0
25	35.06956631443646	36.0	36.0	36.0	36.0	36.0
26	35.02287516250904	36.0	36.0	36.0	36.0	36.0
27	35.01301203783222	36.0	36.0	36.0	36.0	36.0
28	34.995631349733024	36.0	36.0	36.0	36.0	36.0
29	34.97146969270093	36.0	36.0	36.0	36.0	36.0
30	34.949690935814445	36.0	36.0	36.0	36.0	36.0
31	34.959326066958816	36.0	36.0	36.0	36.0	36.0
32	34.93477122008787	36.0	36.0	36.0	36.0	36.0
33	34.91254043632192	36.0	36.0	36.0	32.0	36.0
34	34.898839326225364	36.0	36.0	36.0	32.0	36.0
35	34.892182820263024	36.0	36.0	36.0	32.0	36.0
36	34.87018568743727	36.0	36.0	36.0	32.0	36.0
37	34.86431952133805	36.0	36.0	36.0	32.0	36.0
38	34.84248701907791	36.0	36.0	36.0	32.0	36.0
39	34.82837008951151	36.0	36.0	36.0	32.0	36.0
40	34.81381640806656	36.0	36.0	36.0	32.0	36.0
41	34.79120657798114	36.0	36.0	36.0	32.0	36.0
42	34.76204546971759	36.0	36.0	36.0	32.0	36.0
43	34.758764173220776	36.0	36.0	36.0	32.0	36.0
44	34.709193400747004	36.0	36.0	36.0	32.0	36.0
45	34.69741354632342	36.0	36.0	36.0	32.0	36.0
46	34.675962353345845	36.0	36.0	36.0	32.0	36.0
47	34.653546572346364	36.0	36.0	36.0	32.0	36.0
48	34.62944544957722	36.0	36.0	36.0	32.0	36.0
49	34.61781947664452	36.0	36.0	36.0	32.0	36.0
50	34.203524112437584	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	5.0
20	23.0
21	40.0
22	152.0
23	362.0
24	1027.0
25	2293.0
26	4823.0
27	8867.0
28	14832.0
29	23347.0
30	34073.0
31	49617.0
32	73964.0
33	124080.0
34	283312.0
35	1146774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7088424494619	17.348176293255417	11.643868563858174	26.299112693424515
2	16.78777811597958	19.43035375028713	36.29439700189758	27.48747113183572
3	19.295085398763288	22.560944562935976	27.831454126805422	30.312515911495314
4	13.052092279109342	15.296046490313952	34.21515347981494	37.43670775076177
5	15.131642221007766	36.06054331481098	32.576259027808426	16.231555436372833
6	36.06694082511169	33.9891977273023	15.80212843355731	14.141733014028699
7	31.640297489129292	29.558597732284674	19.346354423017956	19.454750355568077
8	28.94703195417047	32.577503657514114	18.259509819562638	20.215954568752778
9	27.015932391714387	14.311996985733149	18.151962498175486	40.52010812437698
10	17.063486899982067	25.957559234507038	29.991010373994463	26.987943491516432
11	37.995150470074016	21.003635450222166	21.159893052363834	19.84132102733999
12	25.024807732997512	23.139250302954185	27.56051446203144	24.275427502016868
13	29.70235246329191	18.80030142668509	24.872001149585255	26.625344960437747
14	24.030574894461058	18.828984483993498	24.154472124254777	32.98596849729067
15	25.73639648018719	26.436217819250345	21.454587422224787	26.372798278337672
16	26.61951010049293	25.34285441112658	22.642506422597585	25.3951290657829
17	24.735487900502036	25.72768407224736	24.14881471650164	25.388013310748963
18	25.64767674801863	24.522923058384112	25.022870161412143	24.80653003218512
19	26.064412981714124	24.587207243292294	24.656567062345765	24.691812712647813
20	26.292579161457123	23.80953727957275	24.200351891558565	25.69753166741156
21	27.01400887307832	23.704651633802783	24.207312312669085	25.07402718044981
22	26.652274221342687	23.54200116090007	24.502685571460415	25.30303904629683
23	25.041808290600997	23.722838754644737	25.057535901950224	26.17781705280404
24	25.279860509612877	24.205621593854186	24.842708408176843	25.671809488356097
25	25.44345691025634	23.831318348712088	24.812824240915596	25.91240050011598
26	25.26137371689266	24.721201368647826	25.04961574669153	24.96780916776798
27	25.652259266446293	24.173124151739064	24.569087688470216	25.60552889334443
28	25.463068779716174	23.99997510687073	24.990721651817537	25.546234461595557
29	25.162368153664765	24.30419305856342	25.161745836699502	25.371692951072312
30	25.117533975946692	24.45770304383296	25.160530761371234	25.264232218849113
31	25.712837409707262	24.125190089975924	24.31052781649981	25.851444683817004
32	25.689840697631	24.123916174751724	24.19491712407119	25.99132600354609
33	25.12937113774752	24.06175870069343	24.722262924243136	26.086607237315913
34	25.94663646331073	24.103828184845444	24.80507181028913	25.144463541554696
35	26.152417871132865	23.823368341491342	25.01148457672263	25.012729210653163
36	25.011046107385702	24.541254170223766	24.573218578279704	25.874481144110828
37	26.15317421343369	24.161837022419657	24.57676022711586	25.10822853703079
38	25.3107331208411	24.031480079701563	24.858423370977725	25.799363428479616
39	26.1074586030755	24.016241387250272	24.238635470017535	25.637664539656697
40	25.932031749444157	24.219643695653403	24.599765782788996	25.248558772113444
41	24.983720597362144	24.740335748889294	25.06422655723589	25.211717096512672
42	26.208978192423782	24.61430094964371	24.4502278635704	24.726492994362108
43	25.842102255657817	23.775919923553516	24.662703655026757	25.719274165761913
44	25.2822169782232	24.42734832870633	24.53031728582881	25.760117407241662
45	25.3993264080736	24.611903895755624	24.52720986637353	25.46155982979725
46	25.37834856156827	24.351107465135357	24.533619982461598	25.73692399083477
47	25.495142489245133	24.311767780888133	24.868916230852264	25.324173499014467
48	26.13514016587148	24.82980323199886	23.81425200267268	25.220804599456976
49	25.41710843291697	24.950014879554814	24.027539057416977	25.60533763011124
50	25.611296049460385	24.899465702925394	24.180223992903198	25.309014254711027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	170.0
1	114.0
2	58.0
3	180.0
4	302.0
5	324.5
6	347.0
7	272.0
8	197.0
9	292.0
10	387.0
11	586.0
12	785.0
13	1505.5
14	2226.0
15	2825.5
16	3425.0
17	3419.5
18	3414.0
19	3157.5
20	2901.0
21	3316.5
22	3732.0
23	4388.0
24	5044.0
25	6207.0
26	7370.0
27	9320.0
28	11270.0
29	13050.5
30	14831.0
31	17079.5
32	19328.0
33	22726.5
34	26125.0
35	31188.5
36	36252.0
37	44244.0
38	52236.0
39	59724.0
40	67212.0
41	75074.0
42	82936.0
43	87909.0
44	92882.0
45	102519.5
46	112157.0
47	120434.0
48	128711.0
49	135710.5
50	142710.0
51	140588.0
52	138466.0
53	139009.5
54	139553.0
55	140603.5
56	141654.0
57	137237.5
58	132821.0
59	123350.0
60	113879.0
61	100217.5
62	86556.0
63	75281.0
64	64006.0
65	54009.5
66	44013.0
67	37854.5
68	31696.0
69	27600.0
70	23504.0
71	18022.0
72	12540.0
73	10897.5
74	9255.0
75	6789.5
76	4324.0
77	3793.5
78	3263.0
79	2687.0
80	2111.0
81	1695.5
82	1280.0
83	1073.0
84	866.0
85	680.5
86	495.0
87	335.5
88	176.0
89	113.0
90	50.0
91	33.0
92	16.0
93	17.0
94	18.0
95	15.0
96	12.0
97	18.5
98	25.0
99	16.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031738057495103514
2	0.00429962989238479
3	2.2629631012551526E-4
4	0.0
5	0.0
6	1.6972223259413641E-4
7	0.0
8	0.0
9	0.0
10	5.6574077531378816E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.960185427196517E-4
17	0.0
18	3.960185427196517E-4
19	0.0
20	2.2629631012551526E-4
21	0.0
22	0.0
23	1.1314815506275763E-4
24	9.617593180334398E-4
25	2.2629631012551526E-4
26	5.657407753137881E-4
27	3.960185427196517E-4
28	0.002149814946192395
29	3.3944446518827283E-4
30	0.0011314815506275762
31	5.657407753137881E-4
32	6.788889303765457E-4
33	2.8287038765689406E-4
34	0.0011314815506275762
35	3.3944446518827283E-4
36	1.6972223259413641E-4
37	0.002149814946192395
38	0.0
39	6.223148528451669E-4
40	2.2629631012551526E-4
41	0.0013012037832217127
42	0.005035092900292714
43	0.004695648435104441
44	0.004016759504727896
45	0.0031115742642258346
46	0.0024892594113806676
47	0.0012446297056903338
48	0.004582500280041683
49	0.0038470372721337593
50	0.002149814946192395
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1767594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.5394388459118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6700938632187	34.30286699308551
2	16.142174905976344	15.670642233773016
3	5.654541490925326	8.234048127013224
4	2.5821646451432247	5.013472915320204
5	1.348924286312749	3.273801395162145
6	0.8115750530140211	2.3636038592785025
7	0.5348947455643283	1.8174443553923485
8	0.37399654599452803	1.4522865978306907
9	0.2707096766062368	1.1826086213362492
>10	1.4390726272530525	12.430898078713632
>50	0.09414803056889376	3.1574154768243696
>100	0.0672738437203506	6.6434838084958505
>500	0.007617624389288493	2.506087064200847
>1k	0.002812661312968059	1.9513404735735251
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2262	0.12797056337597887	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1965	0.11116806234915938	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1844	0.10432259896786253	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1832	0.10364371003748597	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1829	0.10347398780489185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6972223259413644E-4
2	0.0	0.0	0.0	0.0	1.6972223259413644E-4
3	0.0	0.0	0.0	0.0	1.6972223259413644E-4
4	0.0	0.0	0.0	0.0	1.6972223259413644E-4
5	0.0	0.0	0.0	0.0	1.6972223259413644E-4
6	0.0	0.0	0.0	0.0	2.8287038765689406E-4
7	0.0	0.0	0.0	0.0	2.8287038765689406E-4
8	0.0	0.0	0.0	0.0	2.8287038765689406E-4
9	0.0	0.0	0.0	0.0	2.8287038765689406E-4
10	0.0	0.0	0.0	0.0	2.8287038765689406E-4
11	0.0	0.0	0.0	0.0	2.8287038765689406E-4
12	0.0	0.0	0.0	0.0	5.657407753137881E-4
13	0.0	0.0	0.0	0.0	6.223148528451669E-4
14	0.0	0.0	0.0	0.0	6.223148528451669E-4
15	0.0	0.0	0.0	5.657407753137881E-5	0.0010749074730961974
16	0.0	0.0	0.0	5.657407753137881E-5	0.0010749074730961974
17	0.0	0.0	0.0	1.1314815506275762E-4	0.0010749074730961974
18	0.0	0.0	0.0	1.6972223259413644E-4	0.0010749074730961974
19	0.0	0.0	0.0	2.8287038765689406E-4	0.0011314815506275762
20	0.0	0.0	0.0	4.525926202510305E-4	0.0013577778607530915
21	0.0	0.0	0.0	5.091666977824094E-4	0.0015840741708786068
22	0.0	0.0	0.0	7.920370854393034E-4	0.0016406482484099855
23	0.0	0.0	0.0	9.617593180334398E-4	0.001810370481004122
24	0.0	0.0	0.0	0.0012446297056903338	0.001810370481004122
25	0.0	0.0	0.0	0.001527500093347228	0.001810370481004122
26	0.0	0.0	0.0	0.0019235186360668797	0.0019800927135982583
27	0.0	0.0	0.0	0.0024892594113806676	0.0020366667911296374
28	0.0	0.0	0.0	0.003564166884476865	0.0020366667911296374
29	0.0	0.0	0.0	0.005317963287949608	0.0020366667911296374
30	0.0	0.0	0.0	0.008259815319581306	0.0020366667911296374
31	0.0	0.0	0.0	0.014200093460376081	0.0020366667911296374
32	0.0	0.0	0.0	0.020253519756233615	0.002093240868661016
33	0.0	0.0	0.0	0.027098983137530452	0.002206389023723774
34	0.0	0.0	0.0	0.03383129836376453	0.002319537178786531
35	0.0	0.0	0.0	0.04146879883050067	0.002319537178786531
36	0.0	0.0	0.0	0.052557318026650916	0.002319537178786531
37	0.0	5.657407753137881E-5	0.0	0.06726657818480941	0.002319537178786531
38	0.0	5.657407753137881E-5	0.0	0.08921732026698438	0.0023761112563179102
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2245	0.0	26.856476	1
GGTATCA	955	0.0	23.963198	1
AGTATCG	85	2.3542634E-7	23.293787	8
TTAGTAC	65	4.933875E-4	20.307404	3
GAGCGAA	865	0.0	19.074877	16
TCAACGC	3190	0.0	18.896284	4
CGGATCG	95	1.5979851E-5	18.526054	26
AGCGAAA	880	0.0	18.499737	17
AACGCAG	3250	0.0	18.479738	6
GACGGAC	885	0.0	18.39522	7
CAACGCA	3285	0.0	18.282846	5
ATCAACG	3285	0.0	18.282846	3
GCGAAAG	890	0.0	18.04469	18
CGCAATA	405	0.0	17.92618	36
AGAGCGA	925	0.0	17.837585	15
TATCAAC	3475	0.0	17.409826	2
ACGGACC	960	0.0	17.187258	8
AAGACGG	1005	0.0	17.074385	5
TACACCG	155	1.4406396E-8	17.032017	5
CAAGACG	1055	0.0	16.890755	4
>>END_MODULE