FastQCFastQC Report
Thu 2 Feb 2017
SRR4062326_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062326_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467680
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21260.14485446418837894No Hit
GCGCAAGACGGACCAGAGCGAAAGC15790.10758475962062575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG2800.013.91043954
CGACTTT1051.369699E-913.57116113
GGTATCA5800.013.2620021
CCGATAA1051.9868821E-812.6694429
GTATCAA15250.012.5786371
CGGTCGG853.9332353E-612.29681110
CGATAAC1103.801142E-812.09355810
ACGGTAT2200.012.0935589
GTAATAC550.00307189512.089023
GCGTTGA550.003077393612.0861341
GGTATAT550.003077393612.0861341
CGCATCG2050.012.04854313
CGCAAGA2850.011.9973082
AACCGCG802.8606788E-511.8771967
ATAACGA1052.7163514E-711.76287712
CGGTCCA2350.011.72597410
CGGACCA3000.011.7192349
AAGACGG3000.011.7176355
GTTAGAC658.033462E-411.6904813
TCGCCAG2300.011.564217
Produced by FastQC (version 0.11.5)