##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062326_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1467680
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236103919110434	32.0	32.0	32.0	32.0	32.0
2	30.770171972092008	32.0	32.0	32.0	32.0	32.0
3	30.784961299465824	32.0	32.0	32.0	32.0	32.0
4	30.79973086776409	32.0	32.0	32.0	32.0	32.0
5	30.712315354845742	32.0	32.0	32.0	32.0	32.0
6	34.36380273629129	36.0	36.0	36.0	32.0	36.0
7	34.295029570478576	36.0	36.0	36.0	32.0	36.0
8	34.26049411315818	36.0	36.0	36.0	32.0	36.0
9	34.39180543442712	36.0	36.0	36.0	32.0	36.0
10	34.07593208328791	36.0	36.0	36.0	32.0	36.0
11	34.378855745121555	36.0	36.0	36.0	32.0	36.0
12	34.19658508666739	36.0	36.0	36.0	32.0	36.0
13	34.28919246702279	36.0	36.0	36.0	32.0	36.0
14	34.18845524910062	36.0	36.0	36.0	32.0	36.0
15	34.145160389185655	36.0	36.0	36.0	32.0	36.0
16	34.14628938188161	36.0	36.0	36.0	32.0	36.0
17	34.07921072713398	36.0	36.0	36.0	32.0	36.0
18	34.07382195028889	36.0	36.0	36.0	32.0	36.0
19	34.08994263054617	36.0	36.0	36.0	32.0	36.0
20	34.08192248991606	36.0	36.0	36.0	32.0	36.0
21	34.06619562847487	36.0	36.0	36.0	32.0	36.0
22	34.03529992913987	36.0	36.0	36.0	32.0	36.0
23	33.99992777717213	36.0	36.0	36.0	32.0	36.0
24	33.97315627384716	36.0	36.0	36.0	32.0	36.0
25	33.626712907445764	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	29.0
5	112.0
6	308.0
7	70.0
8	273.0
9	213.0
10	94.0
11	25.0
12	49.0
13	64.0
14	214.0
15	343.0
16	494.0
17	681.0
18	872.0
19	1293.0
20	2044.0
21	2899.0
22	4359.0
23	6528.0
24	9390.0
25	12941.0
26	17679.0
27	22836.0
28	29991.0
29	39292.0
30	51183.0
31	69478.0
32	97120.0
33	137124.0
34	300015.0
35	659666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51836960513576	17.040013790575394	11.578905052842297	25.862711551446544
2	16.984504104780402	18.945900295636854	36.98308690115481	27.086508698427924
3	19.441304544825844	22.172778739620448	28.094072982395897	30.29184373315782
4	13.137113408384252	14.961053792851562	34.624885096593815	37.27694770217037
5	15.268291585758092	36.02468935537363	33.116615355464965	15.590403703403306
6	35.70875359125363	34.19507512486722	16.043273668866984	14.052897615012155
7	31.33057046274899	29.86498948235737	19.723717488752534	19.0807225661411
8	28.508968181644555	33.171699616389915	18.479992797079543	19.83933940488598
9	26.872376217496473	14.553474341675319	18.297749260190542	40.27640018063766
10	16.527895467216837	26.424997373308173	30.75139760472996	26.295709554745034
11	37.5830697390075	21.087364120422805	21.48261914963879	19.8469469909309
12	24.43872869243076	23.40996155512422	28.10446635988088	24.046843392564142
13	29.344461704608328	19.189277140243266	25.019596767096257	26.44666438805214
14	23.471068066070107	19.2714252386494	24.557661269968172	32.699845425312326
15	25.252773343157724	26.887851870045164	21.72595412556115	26.133420661235963
16	26.031603318594033	25.90649434280951	23.015412825511863	25.046489513084598
17	24.44717017301024	26.169768471491615	24.49737936661375	24.885681988884397
18	25.003069501703912	25.308996504860726	25.248015737676294	24.439918255759068
19	25.726121552342065	25.103035023382798	24.83698198600647	24.33386143826867
20	25.729986742393248	24.566740865618062	24.448145299005404	25.255127092983287
21	26.446929165004313	24.288564033840395	24.358945505508782	24.90556129564651
22	25.95395896808394	24.14442950713001	24.73025870200876	25.171352822777287
23	24.69530386420096	24.369280874158942	25.208853150544066	25.726562111096037
24	24.77330943671078	24.975926062322173	24.941758802331833	25.309005698635218
25	25.03341534869257	24.43296199106378	24.870566669758155	25.66305599048549
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	163.0
1	163.0
2	297.0
3	431.0
4	431.0
5	431.0
6	869.0
7	1307.0
8	1307.0
9	1307.0
10	1710.0
11	2113.0
12	2113.0
13	2113.0
14	2887.5
15	3662.0
16	3662.0
17	3662.0
18	6170.0
19	8678.0
20	8678.0
21	8678.0
22	13409.0
23	18140.0
24	18140.0
25	18140.0
26	26622.0
27	35104.0
28	35104.0
29	35104.0
30	44691.5
31	54279.0
32	54279.0
33	54279.0
34	68692.0
35	83105.0
36	83105.0
37	83105.0
38	99304.5
39	115504.0
40	115504.0
41	115504.0
42	133743.5
43	151983.0
44	151983.0
45	151983.0
46	169772.5
47	187562.0
48	187562.0
49	187562.0
50	194427.5
51	201293.0
52	201293.0
53	201293.0
54	191356.5
55	181420.0
56	181420.0
57	181420.0
58	167855.5
59	154291.0
60	154291.0
61	154291.0
62	135716.5
63	117142.0
64	117142.0
65	117142.0
66	95671.0
67	74200.0
68	74200.0
69	74200.0
70	56371.5
71	38543.0
72	38543.0
73	38543.0
74	29167.5
75	19792.0
76	19792.0
77	19792.0
78	15505.0
79	11218.0
80	11218.0
81	11218.0
82	7773.5
83	4329.0
84	4329.0
85	4329.0
86	3138.0
87	1947.0
88	1947.0
89	1947.0
90	1324.0
91	701.0
92	701.0
93	701.0
94	429.0
95	157.0
96	157.0
97	157.0
98	386.5
99	616.0
100	616.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.49482175951161E-4
2	6.813474326828737E-5
3	7.49482175951161E-4
4	0.009743268287365093
5	0.03331788945819252
6	0.06282023329336095
7	0.10710781641774775
8	0.10956066717540608
9	0.12005341763872235
10	0.13340782731930664
11	0.1392674152403794
12	0.13252207565681892
13	0.12788891311457537
14	0.11732802790799084
15	0.13218140194047748
16	0.1197808786656492
17	0.123528289545405
18	0.11201351793306442
19	0.09750081761691921
20	0.09211817289872452
21	0.09436661942657801
22	0.10581325629565029
23	0.0830562520440423
24	0.09293578981794397
25	0.08775754932955412
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1467680.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.28950325076545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.99695818837992	46.04693451122153
2	12.586987014016549	14.673784409417202
3	3.7199095958197863	6.5049504743427295
4	1.581522996429175	3.6874475936607483
5	0.83999526759482	2.4481453440547924
6	0.5165179292721067	1.8064544110431058
7	0.33855397837131734	1.3813900255984133
8	0.2292505027379746	1.0690318339667826
9	0.18019394613594733	0.9453074048153601
>10	0.8880644655086369	9.194650791266657
>50	0.06031379198368615	2.4535906956810427
>100	0.05492543024822867	6.539583498263597
>500	0.004929129777243019	1.8868486726030227
>1k	0.0018777637246640076	1.3618803340650054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2126	0.14485446418837894	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1579	0.10758475962062575	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.3626948653657473E-4
7	0.0	0.0	0.0	0.0	1.3626948653657473E-4
8	6.813474326828737E-5	0.0	0.0	0.0	1.3626948653657473E-4
9	6.813474326828737E-5	0.0	0.0	0.0	2.044042298048621E-4
10	6.813474326828737E-5	0.0	0.0	0.0	2.044042298048621E-4
11	6.813474326828737E-5	0.0	0.0	0.0	2.044042298048621E-4
12	6.813474326828737E-5	0.0	0.0	0.0	4.7694320287801155E-4
13	6.813474326828737E-5	0.0	0.0	6.813474326828737E-5	4.7694320287801155E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	280	0.0	13.9104395	4
CGACTTT	105	1.369699E-9	13.571161	13
GGTATCA	580	0.0	13.262002	1
CCGATAA	105	1.9868821E-8	12.669442	9
GTATCAA	1525	0.0	12.578637	1
CGGTCGG	85	3.9332353E-6	12.296811	10
CGATAAC	110	3.801142E-8	12.093558	10
ACGGTAT	220	0.0	12.093558	9
GTAATAC	55	0.003071895	12.08902	3
GCGTTGA	55	0.0030773936	12.086134	1
GGTATAT	55	0.0030773936	12.086134	1
CGCATCG	205	0.0	12.048543	13
CGCAAGA	285	0.0	11.997308	2
AACCGCG	80	2.8606788E-5	11.877196	7
ATAACGA	105	2.7163514E-7	11.762877	12
CGGTCCA	235	0.0	11.725974	10
CGGACCA	300	0.0	11.719234	9
AAGACGG	300	0.0	11.717635	5
GTTAGAC	65	8.033462E-4	11.690481	3
TCGCCAG	230	0.0	11.5642	17
>>END_MODULE