FastQCFastQC Report
Thu 2 Feb 2017
SRR4062326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467680
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16010.10908372397252808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC250.002352394235.20171740
GGTATCA6600.028.3434071
GTATCAA16450.027.827521
TACACCG755.4759115E-520.5322365
TAATAGG808.993932E-519.248974
GTATTAC1152.0145853E-719.1372381
TCAACGC23550.018.7760684
CAACGCA24250.018.415515
ATCAACG24100.018.3475693
TATCAAC24750.018.1329822
AACGCAG24850.018.0593976
TACGAAT2750.017.6014641
GTTATAT1651.6516424E-917.3394971
ATACGAA2900.016.6904740
CGCAATA2950.016.4075836
CGAATGC3300.016.0007843
ATAGGGG1105.625132E-515.9991443
CGAGCCG5500.015.59969815
TAAACGC2403.6379788E-1215.58356328
TCGAACG3250.015.56999144
Produced by FastQC (version 0.11.5)