##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062326_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1467680
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29471069988008	32.0	32.0	32.0	32.0	32.0
2	31.443296222609835	32.0	32.0	32.0	32.0	32.0
3	31.57029734001962	32.0	32.0	32.0	32.0	32.0
4	31.647291643955086	32.0	32.0	32.0	32.0	32.0
5	31.619800637741196	32.0	32.0	32.0	32.0	32.0
6	35.264157036956284	36.0	36.0	36.0	36.0	36.0
7	35.273987517715035	36.0	36.0	36.0	36.0	36.0
8	35.21867982121444	36.0	36.0	36.0	36.0	36.0
9	35.3047864657146	36.0	36.0	36.0	36.0	36.0
10	35.19048770849231	36.0	36.0	36.0	36.0	36.0
11	35.30726043824267	36.0	36.0	36.0	36.0	36.0
12	35.23376144663687	36.0	36.0	36.0	36.0	36.0
13	35.26272416330535	36.0	36.0	36.0	36.0	36.0
14	35.22794069551946	36.0	36.0	36.0	36.0	36.0
15	35.204963616047095	36.0	36.0	36.0	36.0	36.0
16	35.22016515861768	36.0	36.0	36.0	36.0	36.0
17	35.194444974381334	36.0	36.0	36.0	36.0	36.0
18	35.19509429848468	36.0	36.0	36.0	36.0	36.0
19	35.185554071732255	36.0	36.0	36.0	36.0	36.0
20	35.192013926741524	36.0	36.0	36.0	36.0	36.0
21	35.18140602856209	36.0	36.0	36.0	36.0	36.0
22	35.16723945274174	36.0	36.0	36.0	36.0	36.0
23	35.138928104218905	36.0	36.0	36.0	36.0	36.0
24	35.11542025509648	36.0	36.0	36.0	36.0	36.0
25	35.10421345252371	36.0	36.0	36.0	36.0	36.0
26	35.06391175188052	36.0	36.0	36.0	36.0	36.0
27	35.055100566881066	36.0	36.0	36.0	36.0	36.0
28	35.03194020494931	36.0	36.0	36.0	36.0	36.0
29	35.013586067807694	36.0	36.0	36.0	36.0	36.0
30	34.99771884879538	36.0	36.0	36.0	36.0	36.0
31	35.0018117028235	36.0	36.0	36.0	36.0	36.0
32	34.97613171808569	36.0	36.0	36.0	36.0	36.0
33	34.95494726370871	36.0	36.0	36.0	36.0	36.0
34	34.94512904720375	36.0	36.0	36.0	36.0	36.0
35	34.93319933500491	36.0	36.0	36.0	36.0	36.0
36	34.911869072277334	36.0	36.0	36.0	32.0	36.0
37	34.90087689414586	36.0	36.0	36.0	32.0	36.0
38	34.88776913223591	36.0	36.0	36.0	32.0	36.0
39	34.87947985936989	36.0	36.0	36.0	32.0	36.0
40	34.864228578436716	36.0	36.0	36.0	32.0	36.0
41	34.839426850539624	36.0	36.0	36.0	32.0	36.0
42	34.81170282350376	36.0	36.0	36.0	32.0	36.0
43	34.8048886678295	36.0	36.0	36.0	32.0	36.0
44	34.766917175406086	36.0	36.0	36.0	32.0	36.0
45	34.751938433445986	36.0	36.0	36.0	32.0	36.0
46	34.73511051455358	36.0	36.0	36.0	32.0	36.0
47	34.711929712198845	36.0	36.0	36.0	32.0	36.0
48	34.69195124277772	36.0	36.0	36.0	32.0	36.0
49	34.682896816744794	36.0	36.0	36.0	32.0	36.0
50	34.26962893818816	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	12.0
21	27.0
22	95.0
23	239.0
24	645.0
25	1544.0
26	3274.0
27	6253.0
28	10993.0
29	17419.0
30	26637.0
31	39686.0
32	60372.0
33	102761.0
34	237193.0
35	960526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15484039819685	17.536637463928233	11.97323635134343	26.335285786531482
2	16.439561188334693	19.364063777596073	36.69085581902426	27.50551921504497
3	18.871195004892087	22.623794352432007	28.38228600862861	30.122724634047298
4	12.888776845088849	15.332633816635779	34.79409680584323	36.98449253243214
5	14.921927751231706	36.28879339419587	32.98732215968205	15.801956694890368
6	35.94536956284748	34.43066608525019	15.824225989316473	13.79973836258585
7	31.367873105854137	29.927436498419276	19.604545950070857	19.10014444565573
8	28.68826992259893	33.2315627384716	18.3982884552491	19.68187888368037
9	27.086694647334568	14.311907227733567	18.22393164722555	40.377466477706314
10	16.516077425649613	26.512200556116156	30.779550569300234	26.192171448933998
11	37.99268232857298	20.987613103673823	21.40793633489589	19.611768232857298
12	24.500981140303065	23.33505941349613	28.19306660852502	23.970892837675787
13	29.559100076310912	19.11131854355173	25.01321814019405	26.316363239943314
14	23.614888804098985	19.02247083832988	24.643859696936662	32.71878066063447
15	25.423389294669136	26.870503106944295	21.714202005886843	25.991905592499727
16	26.205156053153527	25.81774005517578	22.944552362821963	25.032551528848728
17	24.479382426687017	26.120271448817178	24.481290199498527	24.919055924997274
18	25.275707614340476	25.10782337815243	25.31617970699295	24.300289300514148
19	25.84861822740652	24.99359533413278	24.910266543115668	24.247519895345036
20	26.00356210379431	24.31129026103925	24.488714099608085	25.196433535558356
21	26.6575820342309	24.245612122533522	24.306388313528835	24.790417529706748
22	26.16524037937425	24.01170554889349	24.787828409462552	25.035225662269706
23	24.805679712198845	24.175160797994113	25.323163087321486	25.695996402485555
24	25.006012927965465	24.74757626198242	24.91082810793427	25.335582702117843
25	25.228949927061706	24.29495822996718	24.97269498303103	25.503396859940082
26	24.969765104672355	25.015415538181134	25.186843136252918	24.827976220893593
27	25.452688468027002	24.601546935427166	24.70974520261965	25.236019393926178
28	25.127703021742732	24.53458321154107	25.135743014574874	25.201970752141328
29	24.976902259081704	24.768681916172234	25.152962915520867	25.1014529092252
30	24.95574606211495	24.797399408312245	25.29587794498203	24.950976584590773
31	25.412319084483336	24.521658079382753	24.569556999715196	25.496465836418714
32	25.374437032847986	24.548229506632964	24.467080474493585	25.61025298602547
33	24.944231484648498	24.445755665086388	24.977004430139118	25.633008420125996
34	25.595676922007716	24.416383108326325	25.090313955976118	24.897626013689838
35	25.860680422654593	24.343315554659203	25.063569888722032	24.73243413396417
36	24.85151665255135	24.866370097426334	24.897712228813912	25.384401021208404
37	25.873579351884658	24.605420947916382	24.70231119348069	24.81868850671827
38	25.101725171699552	24.433391474980922	25.10090755478033	25.36397579853919
39	25.60493843984002	24.545776639162757	24.510482601674763	25.33880231932246
40	25.651148038703337	24.62496857278543	24.876181884706238	24.84770150380499
41	24.89890335262715	25.025363590217047	25.207490861271893	24.868242195883912
42	25.889956391387297	24.852820932134094	24.769623875715453	24.48759880076315
43	25.524985128913414	24.152893167340437	24.950855572960993	25.37126613078516
44	25.099464774241024	24.722183421288427	24.74937057314468	25.42898123132587
45	25.19986181888053	24.939102872027792	24.751454547065048	25.109580762026628
46	25.11050075733986	24.63293034994348	24.87522459572769	25.381344296988967
47	25.177118437351382	24.584475172076402	25.09835370814261	25.140052682429605
48	25.8362189616099	25.195793455790266	24.12732741081126	24.840660171788574
49	25.1613145002487	25.17480563902346	24.407309744281598	25.25657011644624
50	25.21466348152075	25.220386932543683	24.464618852230032	25.10033073370554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	53.0
2	54.0
3	111.5
4	169.0
5	209.5
6	250.0
7	186.0
8	122.0
9	177.0
10	232.0
11	375.5
12	519.0
13	1024.0
14	1529.0
15	1904.0
16	2279.0
17	2273.0
18	2267.0
19	2239.0
20	2211.0
21	2728.5
22	3246.0
23	4075.5
24	4905.0
25	6321.5
26	7738.0
27	9704.0
28	11670.0
29	13551.0
30	15432.0
31	17648.5
32	19865.0
33	23329.5
34	26794.0
35	31341.5
36	35889.0
37	42466.5
38	49044.0
39	55074.5
40	61105.0
41	66942.0
42	72779.0
43	76531.0
44	80283.0
45	87562.5
46	94842.0
47	101840.0
48	108838.0
49	113082.5
50	117327.0
51	115148.5
52	112970.0
53	112183.0
54	111396.0
55	111814.0
56	112232.0
57	108332.5
58	104433.0
59	96093.5
60	87754.0
61	76906.0
62	66058.0
63	56880.0
64	47702.0
65	40416.0
66	33130.0
67	28617.5
68	24105.0
69	21312.0
70	18519.0
71	14591.5
72	10664.0
73	9339.5
74	8015.0
75	5956.5
76	3898.0
77	3373.0
78	2848.0
79	2403.5
80	1959.0
81	1568.5
82	1178.0
83	967.5
84	757.0
85	561.0
86	365.0
87	251.5
88	138.0
89	89.0
90	40.0
91	30.0
92	20.0
93	23.0
94	26.0
95	18.5
96	11.0
97	13.0
98	15.0
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03175079036302191
2	0.0054507794614629896
3	2.7253897307314947E-4
4	0.0
5	6.813474326828737E-5
6	0.0
7	0.0
8	0.0
9	0.0
10	6.813474326828737E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.7694320287801155E-4
17	0.0
18	1.3626948653657473E-4
19	0.0
20	5.450779461462989E-4
21	0.0
22	0.0
23	0.0
24	6.132126894145863E-4
25	6.813474326828737E-5
26	3.4067371634143685E-4
27	2.7253897307314947E-4
28	0.0011582906355608851
29	2.7253897307314947E-4
30	9.538864057560231E-4
31	4.0880845960972423E-4
32	6.813474326828737E-4
33	4.0880845960972423E-4
34	0.001498964351902322
35	2.7253897307314947E-4
36	4.7694320287801155E-4
37	0.0027253897307314948
38	0.0
39	6.813474326828737E-4
40	2.0440422980486211E-4
41	0.0017033685817071842
42	0.0054507794614629896
43	0.004565027798975253
44	0.005110105745121552
45	0.002793524473999782
46	0.0029297939605363566
47	0.0012264253788291726
48	0.004224354082633817
49	0.0034067371634143684
50	0.0023165812711217703
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1467680.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.334709077851414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65329537235857	46.66870398933212
2	12.928521941392706	15.342201763983098
3	3.8001086850313164	6.76435029871649
4	1.6147775864062823	3.8324943325940732
5	0.8164328090095586	2.4221401602097594
6	0.48611416234759924	1.7306065440910938
7	0.34354931621071716	1.426907911188038
8	0.2342021264015314	1.111705202835924
9	0.16992187745666162	0.9074038648368002
>10	0.8403554210217684	8.738259909826068
>50	0.05879234884337084	2.417774998075851
>100	0.048039058670975146	5.84268794519664
>500	0.0046190547836098	1.8685425955654411
>1k	0.0012702400654926952	0.9262204835485914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1601	0.10908372397252808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.813474326828737E-5
7	0.0	0.0	0.0	0.0	6.813474326828737E-5
8	0.0	0.0	0.0	0.0	6.813474326828737E-5
9	0.0	0.0	0.0	0.0	6.813474326828737E-5
10	0.0	0.0	0.0	0.0	1.3626948653657473E-4
11	0.0	0.0	0.0	0.0	1.3626948653657473E-4
12	0.0	0.0	0.0	0.0	2.7253897307314947E-4
13	0.0	0.0	0.0	0.0	3.4067371634143685E-4
14	0.0	0.0	0.0	0.0	4.088084596097242E-4
15	0.0	0.0	0.0	6.813474326828737E-5	4.088084596097242E-4
16	0.0	0.0	0.0	1.3626948653657473E-4	4.7694320287801155E-4
17	0.0	0.0	0.0	2.044042298048621E-4	4.7694320287801155E-4
18	0.0	0.0	0.0	2.044042298048621E-4	6.132126894145863E-4
19	0.0	0.0	0.0	2.7253897307314947E-4	6.132126894145863E-4
20	0.0	0.0	0.0	2.7253897307314947E-4	6.132126894145863E-4
21	0.0	0.0	0.0	4.7694320287801155E-4	6.132126894145863E-4
22	0.0	0.0	0.0	5.450779461462989E-4	6.132126894145863E-4
23	0.0	0.0	0.0	6.813474326828737E-4	8.857516624877357E-4
24	0.0	0.0	0.0	0.0012264253788291726	8.857516624877357E-4
25	0.0	0.0	0.0	0.0014308296086340347	8.857516624877357E-4
26	0.0	0.0	0.0	0.0020440422980486208	9.538864057560231E-4
27	0.0	0.0	0.0	0.0023165812711217703	9.538864057560231E-4
28	0.0	0.0	0.0	0.0030660634470729316	9.538864057560231E-4
29	0.0	0.0	0.0	0.004565027798975253	9.538864057560231E-4
30	0.0	0.0	0.0	0.0067453395835604495	9.538864057560231E-4
31	0.0	0.0	0.0	0.010901558922925979	9.538864057560231E-4
32	0.0	0.0	0.0	0.016352338384388966	0.0010220211490243104
33	0.0	0.0	0.0	0.021258039899705657	0.0010220211490243104
34	0.0	0.0	0.0	0.026776954104436933	0.0010901558922925979
35	0.0	0.0	0.0	0.033931102147607105	0.0010901558922925979
36	0.0	0.0	0.0	0.04251607979941131	0.0010901558922925979
37	0.0	0.0	0.0	0.05593862422326393	0.0010901558922925979
38	0.0	0.0	0.0	0.07378992695955522	0.0011582906355608851
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCGC	25	0.0023523942	35.201717	40
GGTATCA	660	0.0	28.343407	1
GTATCAA	1645	0.0	27.82752	1
TACACCG	75	5.4759115E-5	20.532236	5
TAATAGG	80	8.993932E-5	19.24897	4
GTATTAC	115	2.0145853E-7	19.137238	1
TCAACGC	2355	0.0	18.776068	4
CAACGCA	2425	0.0	18.41551	5
ATCAACG	2410	0.0	18.347569	3
TATCAAC	2475	0.0	18.132982	2
AACGCAG	2485	0.0	18.059397	6
TACGAAT	275	0.0	17.60146	41
GTTATAT	165	1.6516424E-9	17.339497	1
ATACGAA	290	0.0	16.69047	40
CGCAATA	295	0.0	16.40758	36
CGAATGC	330	0.0	16.00078	43
ATAGGGG	110	5.625132E-5	15.999144	3
CGAGCCG	550	0.0	15.599698	15
TAAACGC	240	3.6379788E-12	15.583563	28
TCGAACG	325	0.0	15.569991	44
>>END_MODULE