FastQCFastQC Report
Thu 2 Feb 2017
SRR4062325_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062325_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101611
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC36950.1758175038101723No Hit
GAATAGGACCGCGGTTCTATTTTGT33600.15987735123198346No Hit
GATTAAGAGGGACGGCCGGGGGCAT32920.15664173817133617No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG30320.14427027646886126No Hit
TCGTAGTTCCGACCATAAACGATGC29750.1415580714033187No Hit
ATCAGATACCGTCGTAGTTCCGACC29300.13941685687789035No Hit
GAACTACGACGGTATCTGATCGTCT28640.13627640890726211No Hit
GTATCTGATCGTCTTCGAACCTCCG27710.13185123222137685No Hit
GGGTAGGCACACGCTGAGCCAGTCA27230.12956727006091992No Hit
GTATCAACGCAGAGTACTTTTTTTT27230.12956727006091992No Hit
GAATAACGCCGCCGCATCGCCAGTC26260.1249517631949966No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT25590.12176373267935885No Hit
ACCATACTCCCCCCGGAACCCAAAG23650.11253271894751217No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23390.11129557277726468No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC22820.1085833677117221No Hit
CTATTGGAGCTGGAATTACCGCGGC22450.10682281354636991No Hit
GTGCATGGCCGTTCTTAGTTGGTGG22450.10682281354636991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.013.7043241
TAGATTA802.000832E-613.0622225
CGGTCCA4750.013.00220110
ATCGCCA4450.012.80932616
CGGACCA6750.012.8089669
AAGACGG7300.012.7531525
GTATCAA20750.012.5861941
GGTCCAA5100.012.48250711
CGAACGA1600.012.46907816
GCGTTAT2450.012.40405751
TCGCGTA3000.012.3515029
CGCCAGT4700.012.3292318
TAGGGGC853.946112E-612.2938564
GCCGATT701.0902561E-412.21548112
CGCAAGA6300.012.2116992
CAAGACG7550.012.2050384
CGCATCG4450.011.95565513
CGGCCGA802.8625176E-511.8770110
CGCGTAA3200.011.8770110
GCGTAAC3200.011.8770111
Produced by FastQC (version 0.11.5)