##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062325_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2101611
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29727004664517	32.0	32.0	32.0	32.0	32.0
2	31.01184567458012	32.0	32.0	32.0	32.0	32.0
3	31.030778769239408	32.0	32.0	32.0	32.0	32.0
4	31.030228239193647	32.0	32.0	32.0	32.0	32.0
5	31.02803753882141	32.0	32.0	32.0	32.0	32.0
6	34.57852476029103	36.0	36.0	36.0	32.0	36.0
7	34.54122765821077	36.0	36.0	36.0	32.0	36.0
8	34.52811200550435	36.0	36.0	36.0	32.0	36.0
9	34.60142195677506	36.0	36.0	36.0	32.0	36.0
10	34.435994101667724	36.0	36.0	36.0	32.0	36.0
11	34.54435716219605	36.0	36.0	36.0	32.0	36.0
12	34.469930448593956	36.0	36.0	36.0	32.0	36.0
13	34.53003529197363	36.0	36.0	36.0	32.0	36.0
14	34.45782259419084	36.0	36.0	36.0	32.0	36.0
15	34.41894004171086	36.0	36.0	36.0	32.0	36.0
16	34.42083906108219	36.0	36.0	36.0	32.0	36.0
17	34.371169545648556	36.0	36.0	36.0	32.0	36.0
18	34.378975937982815	36.0	36.0	36.0	32.0	36.0
19	34.36438808133379	36.0	36.0	36.0	32.0	36.0
20	34.35430629169718	36.0	36.0	36.0	32.0	36.0
21	34.31467669326055	36.0	36.0	36.0	32.0	36.0
22	34.29891878182975	36.0	36.0	36.0	32.0	36.0
23	34.288295027005475	36.0	36.0	36.0	32.0	36.0
24	34.262369201531584	36.0	36.0	36.0	32.0	36.0
25	33.86890247529158	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	31.0
5	145.0
6	406.0
7	69.0
8	392.0
9	303.0
10	147.0
11	38.0
12	98.0
13	93.0
14	291.0
15	491.0
16	750.0
17	1103.0
18	1434.0
19	1825.0
20	2612.0
21	3401.0
22	4684.0
23	6354.0
24	8743.0
25	12603.0
26	17654.0
27	23670.0
28	32933.0
29	45223.0
30	61653.0
31	88012.0
32	130581.0
33	196542.0
34	470961.0
35	988365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87058150650483	17.68677379492604	11.53819983222251	25.904444866346626
2	16.922899037308575	19.772241064936942	37.02560134906224	26.27925854869224
3	18.955557670346405	23.362295393985537	28.376522649409974	29.30562428625809
4	12.727556520626	15.536872781539554	36.049092962266606	35.68647773556785
5	15.025638413878681	36.29650219972022	33.3171819020833	15.360677484317801
6	34.238789128245664	35.59064040698851	16.66934088144341	13.501229583322422
7	30.473610102869248	30.172899983042484	20.61181386362727	18.741676050461
8	27.95893354495651	32.79728903854517	19.493895430397046	19.749881986101272
9	26.82878028911771	14.478752063340075	19.121785298300022	39.57068234924219
10	16.467566425376837	26.206000498366016	30.804927820306176	26.521505255950974
11	37.52816034337512	21.404529288663056	21.927616005367142	19.13969436259468
12	24.30409974157449	24.030237309325425	28.429426585047914	23.236236364052175
13	28.635092237790122	19.38834965795242	25.467025127002348	26.509532977255112
14	23.454448882569125	20.10022698745019	24.859293980902567	31.586030149078116
15	25.240408323704933	27.219372744273578	21.910211666051243	25.630007265970246
16	26.034013187386947	25.945214367687136	23.499912106318487	24.52086033860743
17	23.823829689519528	26.16261647603163	25.144625845089912	24.86892798935893
18	24.864967086694964	25.142392498843662	26.020207659399336	23.972432755062037
19	25.412030078052815	25.296960760219545	25.19155993225389	24.09944922947375
20	25.569150025479708	24.50505069747727	24.556581623176534	25.36921765386649
21	26.907992587629	24.385386141952335	24.297708327677853	24.408912942740816
22	25.78782368143545	24.52753441504193	25.02345776506798	24.661184138454637
23	23.96320452281312	24.596701226515908	25.616734256156864	25.823359994514107
24	24.43535543651368	25.37533432901785	25.222314509307875	24.966995725160594
25	24.547768668249393	24.721550847598888	25.20365471209736	25.52702577205436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	174.0
1	174.0
2	439.0
3	704.0
4	704.0
5	704.0
6	1529.0
7	2354.0
8	2354.0
9	2354.0
10	2821.5
11	3289.0
12	3289.0
13	3289.0
14	4665.5
15	6042.0
16	6042.0
17	6042.0
18	9969.5
19	13897.0
20	13897.0
21	13897.0
22	22233.0
23	30569.0
24	30569.0
25	30569.0
26	45834.5
27	61100.0
28	61100.0
29	61100.0
30	76054.0
31	91008.0
32	91008.0
33	91008.0
34	113406.5
35	135805.0
36	135805.0
37	135805.0
38	156582.5
39	177360.0
40	177360.0
41	177360.0
42	201149.5
43	224939.0
44	224939.0
45	224939.0
46	246505.5
47	268072.0
48	268072.0
49	268072.0
50	273492.0
51	278912.0
52	278912.0
53	278912.0
54	257646.0
55	236380.0
56	236380.0
57	236380.0
58	218767.0
59	201154.0
60	201154.0
61	201154.0
62	178642.5
63	156131.0
64	156131.0
65	156131.0
66	127642.0
67	99153.0
68	99153.0
69	99153.0
70	75333.0
71	51513.0
72	51513.0
73	51513.0
74	40037.0
75	28561.0
76	28561.0
77	28561.0
78	24451.5
79	20342.0
80	20342.0
81	20342.0
82	14233.0
83	8124.0
84	8124.0
85	8124.0
86	5990.0
87	3856.0
88	3856.0
89	3856.0
90	2527.0
91	1198.0
92	1198.0
93	1198.0
94	701.5
95	205.0
96	205.0
97	205.0
98	487.0
99	769.0
100	769.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.5648581017134E-4
2	1.4274763502855666E-4
3	5.234079951047078E-4
4	0.009992334451998966
5	0.031499644796301504
6	0.062142803782431665
7	0.10691797863638894
8	0.1085833677117221
9	0.11733855599347358
10	0.13094716386619598
11	0.13870311870274757
12	0.1307092511411484
13	0.12533242355507274
14	0.11305612694261688
15	0.13256497039651963
16	0.11819504180364492
17	0.12247747085450161
18	0.10982051388196959
19	0.0954981678341044
20	0.09045441806309541
21	0.08936001952787648
22	0.09959026670492305
23	0.07989109307098222
24	0.08959793225292408
25	0.08831320353766706
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2101611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.57220284896552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28838821315817	33.666294794879704
2	15.95444704591222	14.860674883306052
3	5.52037775139971	7.712884573233104
4	2.3698609740060834	4.414785840210333
5	1.1839661038103058	2.756995477647646
6	0.6647006884643266	1.8573945178204592
7	0.41433900777235005	1.3507676222749092
8	0.2783999691070256	1.0372559867518503
9	0.18959761620392732	0.7946980777376653
>10	0.9396440300686595	7.784649457870333
>50	0.0829820671662498	2.7300798959172847
>100	0.08861111218770286	8.979940845171884
>500	0.015876515415779224	5.212514069154715
>1k	0.008808905327464603	6.8410639580241375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	3695	0.1758175038101723	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3360	0.15987735123198346	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3292	0.15664173817133617	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3032	0.14427027646886126	No Hit
TCGTAGTTCCGACCATAAACGATGC	2975	0.1415580714033187	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2930	0.13941685687789035	No Hit
GAACTACGACGGTATCTGATCGTCT	2864	0.13627640890726211	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2771	0.13185123222137685	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2723	0.12956727006091992	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2723	0.12956727006091992	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2626	0.1249517631949966	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	2559	0.12176373267935885	No Hit
ACCATACTCCCCCCGGAACCCAAAG	2365	0.11253271894751217	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2339	0.11129557277726468	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2282	0.1085833677117221	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2245	0.10682281354636991	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2245	0.10682281354636991	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	4.7582545009518885E-5
11	0.0	0.0	0.0	0.0	4.7582545009518885E-5
12	0.0	0.0	0.0	0.0	3.806603600761511E-4
13	0.0	0.0	0.0	0.0	3.806603600761511E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	790	0.0	13.704324	1
TAGATTA	80	2.000832E-6	13.062222	5
CGGTCCA	475	0.0	13.002201	10
ATCGCCA	445	0.0	12.809326	16
CGGACCA	675	0.0	12.808966	9
AAGACGG	730	0.0	12.753152	5
GTATCAA	2075	0.0	12.586194	1
GGTCCAA	510	0.0	12.482507	11
CGAACGA	160	0.0	12.469078	16
GCGTTAT	245	0.0	12.4040575	1
TCGCGTA	300	0.0	12.351502	9
CGCCAGT	470	0.0	12.32923	18
TAGGGGC	85	3.946112E-6	12.293856	4
GCCGATT	70	1.0902561E-4	12.215481	12
CGCAAGA	630	0.0	12.211699	2
CAAGACG	755	0.0	12.205038	4
CGCATCG	445	0.0	11.955655	13
CGGCCGA	80	2.8625176E-5	11.87701	10
CGCGTAA	320	0.0	11.87701	10
GCGTAAC	320	0.0	11.87701	11
>>END_MODULE