FastQCFastQC Report
Thu 2 Feb 2017
SRR4062325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101611
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT37800.1798620201359814No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA34950.16630099480826852No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT34130.16239922611748794No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG30340.14436544155888031No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT30110.14327104302366137No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA29460.14017817759804263No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA27480.1307568336861579No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG27170.1292817747908628No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC26050.1239525297497967No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26020.12380978211476813No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC24030.11434085565787389No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA23290.11081974732716948No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC23280.11077216478215997No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG22950.10920194079684585No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT22050.10491951174598915No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG21170.10073224778515148No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT21040.10011367470002773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23750.025.3891121
GGTATCA10800.024.8597431
CGCAATA6550.020.82360636
TCTAGCG7100.019.5208228
CTAGCGG7150.019.3838529
ACCGTCG8100.019.2831888
TAGCGGC7200.019.2492430
ATACCGT8400.019.1182926
TACCGTC8200.019.0480297
CGTCGTA8350.018.70584910
ATACGAA7200.018.63948440
GCGCAAT7400.018.43170535
TACGAAT7350.018.25908741
CCGTCGT8600.018.1620739
CAATACG7750.017.60056338
AATCACG750.001291642717.59930434
TAGGACG1509.61154E-917.5993044
TCAACGC34750.017.1561574
TACGCTA7450.017.126849
ATCAACG34950.017.0579813
Produced by FastQC (version 0.11.5)