##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2101611
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24707188913648	32.0	32.0	32.0	32.0	32.0
2	31.332046225490824	32.0	32.0	32.0	32.0	32.0
3	31.500846731388446	32.0	32.0	32.0	32.0	32.0
4	31.59723897524328	32.0	32.0	32.0	32.0	32.0
5	31.545900264130708	32.0	32.0	32.0	32.0	32.0
6	35.18063761561964	36.0	36.0	36.0	36.0	36.0
7	35.178707667594054	36.0	36.0	36.0	36.0	36.0
8	35.1212027344737	36.0	36.0	36.0	36.0	36.0
9	35.23291037209074	36.0	36.0	36.0	36.0	36.0
10	35.09254234013811	36.0	36.0	36.0	36.0	36.0
11	35.23007350075728	36.0	36.0	36.0	36.0	36.0
12	35.12986799174538	36.0	36.0	36.0	36.0	36.0
13	35.187467138304854	36.0	36.0	36.0	36.0	36.0
14	35.138478053264855	36.0	36.0	36.0	36.0	36.0
15	35.11148352383005	36.0	36.0	36.0	36.0	36.0
16	35.11743705186164	36.0	36.0	36.0	36.0	36.0
17	35.09777499261281	36.0	36.0	36.0	36.0	36.0
18	35.09775262881666	36.0	36.0	36.0	36.0	36.0
19	35.086698251960044	36.0	36.0	36.0	36.0	36.0
20	35.09514129874653	36.0	36.0	36.0	36.0	36.0
21	35.078745781212604	36.0	36.0	36.0	36.0	36.0
22	35.069609456745326	36.0	36.0	36.0	36.0	36.0
23	35.024629676947825	36.0	36.0	36.0	36.0	36.0
24	35.00789870247158	36.0	36.0	36.0	36.0	36.0
25	34.993273731437455	36.0	36.0	36.0	36.0	36.0
26	34.94257119895166	36.0	36.0	36.0	32.0	36.0
27	34.941714713141494	36.0	36.0	36.0	32.0	36.0
28	34.91705125258671	36.0	36.0	36.0	32.0	36.0
29	34.89424684206544	36.0	36.0	36.0	32.0	36.0
30	34.8735303536192	36.0	36.0	36.0	32.0	36.0
31	34.88125775892875	36.0	36.0	36.0	32.0	36.0
32	34.84767399866103	36.0	36.0	36.0	32.0	36.0
33	34.82096829527443	36.0	36.0	36.0	32.0	36.0
34	34.81324993064844	36.0	36.0	36.0	32.0	36.0
35	34.798687292748276	36.0	36.0	36.0	32.0	36.0
36	34.77124025331044	36.0	36.0	36.0	32.0	36.0
37	34.76343528845253	36.0	36.0	36.0	32.0	36.0
38	34.73763888750106	36.0	36.0	36.0	32.0	36.0
39	34.73416964414442	36.0	36.0	36.0	32.0	36.0
40	34.70920213112703	36.0	36.0	36.0	32.0	36.0
41	34.68501354437144	36.0	36.0	36.0	32.0	36.0
42	34.651562063578844	36.0	36.0	36.0	32.0	36.0
43	34.65417767607802	36.0	36.0	36.0	32.0	36.0
44	34.592634412362706	36.0	36.0	36.0	32.0	36.0
45	34.5848227859485	36.0	36.0	36.0	32.0	36.0
46	34.56869039988847	36.0	36.0	36.0	32.0	36.0
47	34.54592595870501	36.0	36.0	36.0	32.0	36.0
48	34.52080380241634	36.0	36.0	36.0	32.0	36.0
49	34.50273385512352	36.0	36.0	36.0	32.0	36.0
50	34.05818155691039	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	22.0
21	53.0
22	115.0
23	359.0
24	930.0
25	2221.0
26	4962.0
27	9468.0
28	16933.0
29	27776.0
30	42400.0
31	64344.0
32	99896.0
33	174652.0
34	395221.0
35	1262256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30509729035845	18.37500517615835	11.992266462444414	26.327631071038788
2	16.294902236962947	20.297452783950586	36.71526664160532	26.69237833748114
3	18.365547540003874	23.846443367914773	28.58167242336705	29.206336668714307
4	12.421001042058231	16.008821808042406	36.218042358001725	35.35213479189764
5	14.668509062809434	36.75908624383866	33.01453028176955	15.557874411582354
6	34.42285035620412	35.803938325203035	16.444439580434754	13.328771738158093
7	30.458824206763286	30.261023567158716	20.61989587987501	18.66025634620298
8	28.04924412748125	32.91603441359985	19.32731604469143	19.707405414227466
9	27.074039867511164	14.181692044817048	18.95450680454185	39.78976128312994
10	16.433115563782053	26.187922592678948	30.81865807642712	26.560303767111883
11	37.75950925266379	21.264258704393914	21.853711271971836	19.12252077097046
12	24.36316711322885	23.953671730876934	28.375517638611527	23.307643517282695
13	28.781016087182643	19.250898477406142	25.42606600365148	26.542019431759734
14	23.570394330825255	19.913009591213598	24.769997873060237	31.746598204900906
15	25.35997384863326	27.151028425336566	21.764541582624	25.724456143406176
16	26.16541024239163	25.806873718535268	23.354693259751265	24.67302277932184
17	24.016385525199478	26.20913194687314	24.982977344522844	24.791505183404542
18	25.039826647024565	25.025218784854264	25.85991298091747	24.075041587203703
19	25.65898256147308	25.083709592307997	25.10902350625306	24.148284339965866
20	25.86695520763149	24.284928492849282	24.457796125633323	25.3903201738859
21	27.102970054876952	24.292935276794804	24.258628261842937	24.34546640648531
22	25.91107488493351	24.509150361318056	24.943245919439896	24.63652883430854
23	24.10033260214788	24.405812686464188	25.584718382573364	25.909136328814576
24	24.66939348836501	25.175628761434186	25.20517778902851	24.949799961172292
25	24.86773216876419	24.483787882320527	25.10298071904024	25.54549922987504
26	24.473175951880542	25.748678508738333	25.58309062177776	24.195054917603372
27	25.4130299585602	24.621158230170018	24.879199354016908	25.086612457252865
28	24.513378724513853	24.82266938779006	25.683258762561362	24.98069312513472
29	24.49365983362208	24.85129194361822	25.65929498510897	24.995753237650725
30	24.389342144456617	25.210674224133584	25.894110448014025	24.505873183395778
31	25.300604015413004	24.91627815352288	24.598044729687164	25.185073101376947
32	25.056302367863708	24.68306275364615	24.626058539236077	25.63457633925406
33	24.36140534362514	24.461804704685512	25.507290373509417	25.669499578179934
34	25.338980470080834	24.736819065129552	25.46607435491883	24.458126109870783
35	26.100205176226964	24.25633134247681	25.43999642178751	24.203467059508718
36	24.335021405028243	25.21391943030316	24.983810023653305	25.46724914101529
37	25.63297794890877	25.058932019524416	24.93692822617714	24.371161805389676
38	24.58709057004365	24.523187212095866	25.50072301677142	25.388999201089067
39	25.625403858580263	24.514774919323447	24.622502262559703	25.23731895953658
40	25.595199859155265	24.843203170909852	25.33482742951221	24.226769540422676
41	24.359890635393896	25.49284019653653	25.75954251867868	24.387726649390892
42	26.198768409967876	25.42356428303254	24.822853651491712	23.55481365550787
43	25.278988010605634	24.39082304080277	25.271945502406822	25.05824344618477
44	24.734206187382078	25.105030370657637	24.85397578436714	25.306787657593137
45	25.01412042929912	25.50090051937485	24.879982298905816	24.60499675242022
46	24.652532094000605	25.20350349097647	24.90643859493015	25.237525820092777
47	24.730048986836124	24.86456650575637	25.598060511471104	24.8073239959364
48	25.877100735370394	25.544580462007133	24.211596715910247	24.36672208671223
49	24.653999943850085	25.72403649363769	24.51686091523409	25.105102647278134
50	25.13535067458833	25.52286900605164	24.702247549222538	24.63953277013749
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	49.5
2	31.0
3	89.0
4	147.0
5	175.5
6	204.0
7	189.5
8	175.0
9	245.5
10	316.0
11	459.5
12	603.0
13	1139.5
14	1676.0
15	2185.5
16	2695.0
17	3079.0
18	3463.0
19	3834.0
20	4205.0
21	5263.0
22	6321.0
23	7334.5
24	8348.0
25	10893.5
26	13439.0
27	17055.5
28	20672.0
29	24477.5
30	28283.0
31	31539.0
32	34795.0
33	39371.0
34	43947.0
35	50981.5
36	58016.0
37	69619.0
38	81222.0
39	89643.5
40	98065.0
41	106139.0
42	114213.0
43	116313.5
44	118414.0
45	128685.0
46	138956.0
47	147709.0
48	156462.0
49	161673.5
50	166885.0
51	156720.5
52	146556.0
53	144724.5
54	142893.0
55	146333.0
56	149773.0
57	145571.5
58	141370.0
59	127943.0
60	114516.0
61	100404.0
62	86292.0
63	74155.0
64	62018.0
65	52938.0
66	43858.0
67	39045.0
68	34232.0
69	32455.5
70	30679.0
71	23650.0
72	16621.0
73	14841.5
74	13062.0
75	9352.5
76	5643.0
77	5011.5
78	4380.0
79	3876.5
80	3373.0
81	2654.0
82	1935.0
83	1669.5
84	1404.0
85	1110.5
86	817.0
87	570.5
88	324.0
89	213.0
90	102.0
91	74.0
92	46.0
93	40.0
94	34.0
95	27.5
96	21.0
97	22.0
98	23.0
99	20.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03007216844601594
2	0.004805837045961408
3	9.516509001903777E-5
4	4.7582545009518885E-5
5	0.0
6	3.330778150666322E-4
7	0.0
8	0.0
9	0.0
10	4.7582545009518885E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.709905401142266E-4
17	0.0
18	1.4274763502855666E-4
19	0.0
20	1.4274763502855666E-4
21	0.0
22	0.0
23	4.7582545009518885E-5
24	9.040683551808589E-4
25	1.9033018003807554E-4
26	3.806603600761511E-4
27	3.806603600761511E-4
28	0.001332311260266529
29	4.758254500951889E-4
30	0.0011895636252379721
31	4.2824290508567E-4
32	5.709905401142266E-4
33	1.9033018003807554E-4
34	7.137381751427833E-4
35	1.4274763502855666E-4
36	9.516509001903777E-5
37	0.0017605541653521986
38	0.0
39	4.2824290508567E-4
40	2.854952700571133E-4
41	8.08903265161821E-4
42	0.0047582545009518884
43	0.004139681415828143
44	0.0036162734207234354
45	0.002188797070437869
46	0.0023791272504759442
47	0.0012371461702474912
48	0.004044516325809106
49	0.004472759230894775
50	0.0015702239853141234
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2101611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.312962751761916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28214178010994	34.92164423440025
2	16.124502161956254	15.580449446825941
3	5.498989111288135	7.970173683180241
4	2.362574280743714	4.565718528953671
5	1.1627825233109153	2.8088734368559987
6	0.6660343856259737	1.9306856678484177
7	0.41842218626835487	1.415065084978567
8	0.26353753479718955	1.0185823281878224
9	0.18203329029545942	0.7915110816262675
>10	0.8655467431025832	7.322304285880585
>50	0.07177836423738798	2.44963328202487
>100	0.08037830262930443	8.488075907860576
>500	0.013163495997391713	4.401134128813509
>1k	0.008115839637489628	6.336148902563211
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3780	0.1798620201359814	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3495	0.16630099480826852	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3413	0.16239922611748794	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	3034	0.14436544155888031	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	3011	0.14327104302366137	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2946	0.14017817759804263	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2748	0.1307568336861579	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2717	0.1292817747908628	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2605	0.1239525297497967	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2602	0.12380978211476813	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2403	0.11434085565787389	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2329	0.11081974732716948	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC	2328	0.11077216478215997	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	2295	0.10920194079684585	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	2205	0.10491951174598915	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	2117	0.10073224778515148	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	2104	0.10011367470002773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.7582545009518885E-5	0.0
5	0.0	0.0	0.0	4.7582545009518885E-5	0.0
6	0.0	0.0	0.0	4.7582545009518885E-5	9.516509001903777E-5
7	0.0	0.0	0.0	4.7582545009518885E-5	9.516509001903777E-5
8	0.0	0.0	0.0	4.7582545009518885E-5	9.516509001903777E-5
9	0.0	0.0	0.0	4.7582545009518885E-5	9.516509001903777E-5
10	0.0	0.0	0.0	4.7582545009518885E-5	9.516509001903777E-5
11	0.0	0.0	0.0	4.7582545009518885E-5	9.516509001903777E-5
12	0.0	0.0	0.0	4.7582545009518885E-5	2.3791272504759443E-4
13	0.0	0.0	0.0	4.7582545009518885E-5	2.3791272504759443E-4
14	0.0	0.0	0.0	4.7582545009518885E-5	2.3791272504759443E-4
15	0.0	0.0	0.0	4.7582545009518885E-5	3.806603600761511E-4
16	0.0	0.0	0.0	4.7582545009518885E-5	3.806603600761511E-4
17	0.0	0.0	0.0	4.7582545009518885E-5	3.806603600761511E-4
18	0.0	0.0	0.0	4.7582545009518885E-5	3.806603600761511E-4
19	0.0	0.0	0.0	4.7582545009518885E-5	3.806603600761511E-4
20	0.0	0.0	0.0	1.9033018003807554E-4	4.7582545009518885E-4
21	0.0	0.0	0.0	1.9033018003807554E-4	4.7582545009518885E-4
22	0.0	0.0	0.0	2.3791272504759443E-4	4.7582545009518885E-4
23	0.0	0.0	0.0	4.2824290508567E-4	4.7582545009518885E-4
24	0.0	0.0	0.0	7.613207201523022E-4	4.7582545009518885E-4
25	0.0	0.0	0.0	9.516509001903777E-4	4.7582545009518885E-4
26	0.0	0.0	0.0	0.0010468159902094155	5.234079951047078E-4
27	0.0	0.0	0.0	0.0011419810802284533	5.234079951047078E-4
28	0.0	0.0	0.0	0.0014274763502855666	5.234079951047078E-4
29	0.0	0.0	0.0	0.0024742923404949824	5.234079951047078E-4
30	0.0	0.0	0.0	0.004615506865923332	5.234079951047078E-4
31	0.0	0.0	0.0	0.009135848641827626	5.234079951047078E-4
32	0.0	0.0	0.0	0.01413201586782711	6.185730851237456E-4
33	0.0	0.0	0.0	0.019556425998912264	6.185730851237456E-4
34	0.0	0.0	0.0	0.025123583765025975	6.185730851237456E-4
35	0.0	0.0	0.0	0.03164239243133006	6.185730851237456E-4
36	0.0	0.0	0.0	0.04058791089311961	6.185730851237456E-4
37	0.0	0.0	0.0	0.053720693315746824	6.185730851237456E-4
38	0.0	0.0	0.0	0.07170689532934496	6.661556301332645E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2375	0.0	25.389112	1
GGTATCA	1080	0.0	24.859743	1
CGCAATA	655	0.0	20.823606	36
TCTAGCG	710	0.0	19.52082	28
CTAGCGG	715	0.0	19.38385	29
ACCGTCG	810	0.0	19.283188	8
TAGCGGC	720	0.0	19.24924	30
ATACCGT	840	0.0	19.118292	6
TACCGTC	820	0.0	19.048029	7
CGTCGTA	835	0.0	18.705849	10
ATACGAA	720	0.0	18.639484	40
GCGCAAT	740	0.0	18.431705	35
TACGAAT	735	0.0	18.259087	41
CCGTCGT	860	0.0	18.162073	9
CAATACG	775	0.0	17.600563	38
AATCACG	75	0.0012916427	17.599304	34
TAGGACG	150	9.61154E-9	17.599304	4
TCAACGC	3475	0.0	17.156157	4
TACGCTA	745	0.0	17.12684	9
ATCAACG	3495	0.0	17.057981	3
>>END_MODULE